LOCUS       sequence-412578-        6025 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Induces NM-like pigment accumulation in catecholaminergic neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6025)
  TITLE     Prigge Lab AAV vectors
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV_Cbh_DIO_hTyr
FEATURES             Location/Qualifiers
     source          1..6025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        145..430
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          655..882
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     protein_bind    complement(912..945)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(996..1029)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1041..1067)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2693..2726
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    2777..2810
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2880..2900)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     repeat_region   3288..3428
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3288..3417
                     /label=AAV2 ITR
     rep_origin      3503..3958
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3590..3609)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3800..3821
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3975..3994)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4094..4116
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4154..4172)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4240..4344
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4345..4413,4414..5205)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4563..4582)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5376..5964
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5865..5884
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgttac ataacttacg gtaaatggcc cgcctggctg
      181 accgcccaac gacccccgcc cattgacgtc aatagtaacg ccaataggga ctttccattg
      241 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      301 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattgtgc
      361 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      421 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
      481 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
      541 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
      601 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
      661 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
      721 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
      781 tgtaattagc tgagcaagag gtaagggttt aagggatggt tggttggtgg ggtattaatg
      841 tttaattacc tggagcacct gcctgaaatc actttttttc aggttgggga tcctctagag
      901 tcgactccgg aataacttcg tataggatac tttatacgaa gttatgcaga atggtagctg
      961 gattgtagct gctattagca atatgaaacc tcttaataac ttcgtatagc atacattata
     1021 cgaagttatg gcgcgcctta agcgtaatct ggaacatcgt atgggtaaaa ctccccggac
     1081 ccagctgcta aatggctctg atacaagctg tggtaatcct ctttctccat gaggagtggc
     1141 tgcttttctt caggaagctg ctttctcttg tgacgacaca gcaagctcac aagccctgcc
     1201 agcagggcag tgaggacggc ccctaccatc gccgccccaa ggagccatga ccagatccga
     1261 ctcgcttgtt ccaaatagga cttaatgtag tcttgaaaag agtctgggtc tgaatcttgt
     1321 agatagctat agtcatagcc cagatctttg gatgaaataa agaaatcacc atttctgtac
     1381 agtggtataa aaggaaccat gtaggattcc cggttatgtc caatgggtgc attggcttct
     1441 ggataaactt cttgaagagg acggtgcctt cggagccact gctcaaaaat actgtcaaca
     1501 aatgcatggt gaagaaggaa gataggatcg ttggcagatc cctgtacctg ggacattgtt
     1561 ccattcatat agatgtgcaa ggcattgtgc atgctgcttt gagaggcatc cgctatccca
     1621 gtaagtggac tagcaaatcc ttccagtgta tttctaaagc tgaaattggc agctttatcc
     1681 atggaaccag attcatattg ggtcaaactc aggcaaaatt ctacatcagc tgaagagggg
     1741 agccttgggg ttctggattt gtcatggttt ccaggattac gccgtaaagg tccctcgggc
     1801 gttccattgc ataaagactg atggctgttg tactcctcca atcggctaca gacaatctgc
     1861 caagaggaga agaatgatgc tgggctgagt aagttaggat ttgtggggtg ctgacctccc
     1921 atgtactcat ctgtgcaaat gtcacacttt tctgcatccc gccagtccca atatggaata
     1981 gtgaagtttt catctcctgt cagcttctgg atttcttgtt cccaccgcaa caagaagagt
     2041 ctatgccaag gcagaaaagc tggtgcttca tgggcaaaat caatgtctct ccagatttca
     2101 gatcccccaa gcagtgcatc cattgacaca taataatgca tccagacaaa gaggtcataa
     2161 atattgatgt cgttaaacat gggtgttgat ccatttttca tttggccata ggtccctatg
     2221 gggatgacat agtctgagct gatggtatgc tttgctaaag tgaggtaggc aaaaaatttg
     2281 tccttctctg gggcactcaa atcgaagatg tttcttctca ccaagagtcg tctctctgtg
     2341 cagtttggtc cccaaaagcc aaacttgcag tttccacagt tgaatcccat gaagttgcca
     2401 gagcactggc aggtcctatt ataaaagacg gaaggccacg actcccggtc atccacccct
     2461 gtgaagggaa attgaggccc aagtggtgca ttggacagaa ggatattctg acaggaacct
     2521 ctgcctgaaa gctggccaca gggactcctg tccccgctcc acggtggaca gcattccttc
     2581 tccatcaggt tcttagagga gacacaggct ctagggaaat ggccagcgga ggtctggaaa
     2641 ctccacagca ggcagtacaa aacagccagg agcatcaagg tcttaggcta gcataacttc
     2701 gtataaagta tcctatacga agttatttgc cttaacccag aaattatcac tgttattctt
     2761 tagaatggtg caaagaataa cttcgtataa tgtatgctat acgaagttat gaattccgct
     2821 cgagataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     2881 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     2941 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttagttct tgccacggcg
     3001 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3061 aattccgtgg tgtttatttg tgaaatttgt gatgctattg ctttatttgt aaccatctag
     3121 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     3181 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga
     3241 ggttttttaa agcggccgcg gtaaccacgt gcggaccgag cggccgcagg aacccctagt
     3301 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
     3361 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
     3421 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     3481 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     3541 gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct
     3601 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     3661 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     3721 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     3781 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc
     3841 tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat
     3901 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
     3961 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     4021 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     4081 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     4141 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     4201 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4261 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4321 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4381 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4441 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4501 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4561 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     4621 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4681 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4741 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4801 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4861 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     4921 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4981 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5041 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5101 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5161 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5221 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5281 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5341 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5401 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5461 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5521 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5581 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5641 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5701 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5761 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5821 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5881 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5941 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6001 ttttgctggc cttttgctca catgt
//
