LOCUS       sequence-412877-       13750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tetracycline inducible expression vector for HA-JLP_V55Q mutant 
            protein, used as a donor plasmid for HA-JLP_V55Q knock-in at the 
            human ROSA26 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13750)
  AUTHORS   Suzuki R, Kanemaki MT, Suzuki T, Yoshioka K
  TITLE     Overexpression of JNK-associated leucine zipper protein induces 
            chromosomal instability through interaction with dynein light 
            intermediate chain 1.
  JOURNAL   Genes Cells. 2023 Nov 14. doi: 10.1111/gtc.13083.
  PUBMED    37963657
REFERENCE   2  (bases 1 to 13750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Cells. 2023 Nov 14. doi: 10.1111/gtc.13083."
COMMENT     Sequence Label: phROSA26-Tet-HA-JLP_V55Q
FEATURES             Location/Qualifiers
     source          1..13750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2323..2404
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2521..3267)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(3286..3796)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3349..3367)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     polyA_signal    3816..3864
                     /note="synthetic polyadenylation signal"
     misc_feature    3878..3969
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3918..3937
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        3973..4340
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    3981..3999
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4017..4035
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4053..4071
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4089..4107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4125..4143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4161..4179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4197..4215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             4355..4381
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    8484..8565
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    8615..8648
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        8745..9102
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8745..8936
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8768..8788)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8953..9088
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9137..9931
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9137..9931
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9191..9210)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9801..9820
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    10437..10470
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(11562..11582)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11562..11580)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11601..11617)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11601..11617)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11614..11636)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11625..11641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11649..11655,11656..11673,11674..11679))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11694..11715
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(12003..12591)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12083..12102)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12762..13553,13554..13622))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13385..13404
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13623..13727)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcag
      661 tggcagcatc ctcctaagag cttggggagg gccaggccca cgacccaagg agagcgagcg
      721 cggggagacg gaggaggtga cccttccctc ccctggggcc cgatcgtgag gttcggtctc
      781 ttttctgtcg gacccttacc ttgtcccagg cgctgccggg gcctgggccc gggctgcggc
      841 gcacggcact cccgggaggc ggcaggactc gagttaggcc caacgcggcg ccacggcgtt
      901 tcctggccgg gaatggcccg tacccgtgag gtgggggtgg ggggcagaaa ggcggagcga
      961 gccaaaggcg gggagggggg gcagggccag ggaaagaggg gggccggcac tactgtgttg
     1021 gcggactggc gggactgggg ctgcgtgagt ctctgagcgc aggcgggcgg cggccgcccc
     1081 tcccccggca gcggcggcgg cggcggcggc ggcggcggcg gcagctcact cagcccgctg
     1141 cccgagcgga aacgccactg accgcacggg gattcccagc gccggcgcca ggggcacccg
     1201 ggacacgccc cctcccgccg cgccattggc ctctccgccc accgccccgc acccattggc
     1261 ccactcgccg ccaatcagcg gaagccgccg gggccgccta gagaagaggc tgtgcttcgg
     1321 cgctccggct cctcagagag cctcggctag gtaggggagc ggaactctgg tgggagggga
     1381 ggtgcggtgc actgggggga tgggtggcta ggggggccgt ctggtggctt gcgggggttg
     1441 cctttcccgt gggaagtcgg gaacataatg tttgttacgt tgggagggaa aggggtggct
     1501 ggatgcaggc gggagggagg cccgccctgc ggcaaccgga gggggaggga gaagggagcg
     1561 gaaaatgctc gaaaccggac ggagccattg ctctcgcaga gggaggagcg cttccggcta
     1621 gcctcttgtc gccgattggc cgtttctcct cccgccgtgt gtgaaaacac aaatggcgta
     1681 ttctggttgg agtaaagctc ctgtcagtta cgccgtcggg agtacgcagc cgcttagcga
     1741 ctctcgcgtt gccccctggg tggggcgggt aggtaggtgg ggtgtagaga tgctgggtgt
     1801 gcgggcgcgg ccggcctcct gcggcgggag gggagggtca gtgaaattgg ctctggcgcg
     1861 ggcgtcctcc caccctcccc ttccttcggg ggagtcggtt tacccgccgc ctgcttgtct
     1921 tcgacacctg attggctgtc gaagctgtgg gaccgggccc ttgctactgg ctcgagtctc
     1981 acatgagcga aaccactgcg cggggcgcgg gggtggcggg gaggcgggcg ttggtacggt
     2041 cctccccgag gccgagcgcc gcagtgtctg gccccgcgcc cctgcgcaac gtggcaggaa
     2101 gcgcgcgctg gaggcggggg cgggctgccg gccgagactt ctggatggcg gcggccgcgg
     2161 ctccgccccg ggttcccacc gcctgaaggg cgagacaagc ccgacctgct acaggcactc
     2221 gtgggggtgg gggaggagcg ggggtcggtc cggctggttt gtgggtggga ggcgcttgtt
     2281 ctccaaaaac cggcgcggag tcagtgagcg aggaagctcg gggcagtgaa aaaaatgctt
     2341 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     2401 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     2461 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctggagat
     2521 ttacccgggg agcatgtcaa ggtcaaaatc gtcaagagcg tcagcaggca gcatatcaag
     2581 gtcaaagtcg tcaagggcat cggctgggag catgtctaag tcaaaatcgt caagggcgtc
     2641 ggtcggcccg ccgctttcgc actttagctg tttctccagg ccacatatga ttagttccag
     2701 gccgaaaagg aaggcaggtt cggctccctg ccggtcgaac agctcaattg cttgtttcag
     2761 aagtgggggc atagaatcgg tggtaggtgt ctctctttcc tcttttgcta cttgatgctc
     2821 ctgttcctcc aatacgcagc ccagtgtaaa gtggcccacg gcggacagag cgtacagtgc
     2881 gttctccagg gagaagcctt gctgacacag gaacgcgagc tgattttcca gggtttcgta
     2941 ctgtttctct gttgggcggg tgccgagatg cactttagcc ccgtcgcgat gtgagaggag
     3001 agcacagcgg tatgacttgg cgttgttccg cagaaagtct tgccatgact cgccttccag
     3061 ggggcaggag tgggtatgat gcctgtccag catctcgatt ggcagggcat cgagcagggc
     3121 ccgcttgttc ttcacgtgcc agtacagggt aggctgctca actcccagct tttgagcgag
     3181 tttccttgtc gtcaggcctt cgataccgac tccattgagt aattccagag cagagtttat
     3241 gactttgctc ttgtccagtc tagacatggt aattcgatga tccccctggg gagagaggtc
     3301 ggtgattcgg tcaacgaggg agccgactgc cgacgtgcgc tccggaggct tgcagaatgc
     3361 ggaacaccgc gcgggcagga acagggccca cactaccgcc ccacaccccg cctcccgcac
     3421 cgccccttcc cggccgctgc tctcggcgcg ccctgctgag cagccgctat tggccacagc
     3481 ccatcgcggt cggcgcgctg ccattgctcc ctggcgctgt ccgtctgcga gggtactagt
     3541 gagacgtgcg gcttccgttt gtcacgtccg gcacgccgcg aaccgcaagg aaccttcccg
     3601 acttaggggc ggagcaggaa gcgtcgccgg ggggcccaca agggtagcgg cgaagatccg
     3661 ggtgacgctg cgaacggacg tgaagaatgt gcgagaccca gggtcggcgc cgctgcgttt
     3721 cccggaacca cgcccagagc agccgcgtcc ctgcgcaaac ccagggctgc cttggaaaag
     3781 gcgcaacccc aaccccgtgg aattatcacc tcgagaataa aatatcttta ttttcattac
     3841 atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca
     3901 aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa
     3961 catttctctc tcgagtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc
     4021 tatcagtgat agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag
     4081 gagtttactc cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag
     4141 agaacgtatc tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc
     4201 tatcagtgat agagaacgta taagctttgc ttatgtaaac cagggcgcct ataaaagagt
     4261 gctgattttt tgagtaaact tcaattccac aacacttttg tcttatacca actttccgta
     4321 ccacttccta ccctcgtaaa gaattgccac catgtacccc tatgatgtgc cagactacgc
     4381 cgatatcgag ctggaggacg gtgtggtgta tcaggaggag cccggcggct ccggggccgt
     4441 gatgtcggag cgggtgtccg gcctggccgg ctccatctac cgcgagttcg agcggcttat
     4501 cgggcgctat gacgaggagg tggtcaaaga gctgatgccg ctggtgcagg ctgtgctgga
     4561 gaacctggac tcggtgttcg cgcaggacca ggagcaccag gtggagctgg agctgctgcg
     4621 ggacgacaac gagcagctca tcacccagta cgagcgggag aaggcgctgc gcaagcacgc
     4681 tgaggagaaa ttcattgaat ttgaagactc tcaagaacag gaaaaaaagg acttacagac
     4741 ccgagtggaa tctttagaat ctcaaacaag acaacttgag ctgaaagcga aaaactatgc
     4801 tgaccagatt agcagacttg aagaaagaga agcagaactg aagaaggaat ataatgcatt
     4861 acatcaaaga cacactgaga tgatccataa ttatatggaa catttagaaa gaacaaaact
     4921 tcatcagctc tcagggagtg atcaactaga atccacagct catagtagaa ttagaaaaga
     4981 acgccctata tcattaggaa ttttcccatt acctgctgga gatggattgc ttacacctga
     5041 tgctcagaaa ggaggagaga cccctggatc tgagcaatgg aaatttcagg aattaagtca
     5101 accacgttct cataccagcc tgaaggatga gctttctgat gttagccaag gcggatctaa
     5161 agctaccact ccagcatcaa cagctaattc agatgtggca acaattccta ctgatactcc
     5221 cttaaaggaa gaaaacgaag gatttgtgaa ggttacagat gcgccaaata aatcagagat
     5281 aagcaaacac attgaagtac aggtagccca ggaaactaga aatgtatcta ctggctctgc
     5341 tgaaaatgaa gaaaagtcag aagttcaagc aatcatcgaa tctactcctg agctggatat
     5401 ggacaaagat cttagtggat ataaaggttc aagcactccc accaaaggca tagagaacaa
     5461 agcttttgat cgcaatacag aatctctctt tgaagaactg tcttcagctg gctcaggcct
     5521 aataggagat gtggatgaag gagcagattt actaggaatg ggtcgggaag ttgagaatct
     5581 tatattagaa aatacacaac tgttggaaac caaaaatgct ttgaacatag tgaagaatga
     5641 tttgatagca aaagtggatg aactgacctg tgagaaagat gtgctgcaag gggaattgga
     5701 ggctgtgaag caagccaaac tgaaactaga ggaaaagaac agagaattgg aggaagagct
     5761 taggaaagct cgggcagaag ctgaagatgc aaggcaaaaa gcaaaagatg acgatgatag
     5821 tgatattccc acagcccaga ggaaacggtt tactagagta gaaatggccc gtgttctcat
     5881 ggagcgaaac cagtataaag agagattgat ggagcttcag gaagctgttc gatggacaga
     5941 gatgattcgg gcatcacgag aaaatccagc catgcaggaa aaaaaaaggt caagcatttg
     6001 gcagtttttc agccgacttt tcagctcctc aagtaacacg actaagaagc ctgaaccacc
     6061 tgttaatctg aagtacaatg cacccacgtc tcatgttact ccgtccgtca agaaaagaag
     6121 cagcacctta tctcagctcc ctggggataa gtccaaagcc tttgatttcc ttagtgaaga
     6181 aactgaagct agtttagcct cacgcagaga acaaaagaga gagcagtatc gtcaggtaaa
     6241 agcacatgtt cagaaggaag acggtagagt gcaggctttt ggctggagtc tgcctcagaa
     6301 gtacaaacag gtaaccaatg gtcaaggtga aaataagatg aaaaatttac ctgtgcctgt
     6361 ctatctcaga cctctggatg aaaaagatac atcaatgaag ctgtggtgtg ctgttggagt
     6421 caatttatct ggtgggaaga ccagagatgg tggttctgtt gttggagcaa gtgtatttta
     6481 caaggatgtt gctggtttgg atacagaagg cagtaaacag cgaagtgcct ctcagagtag
     6541 tttagataag ttagatcagg aacttaagga acagcagaag gagttaaaaa atcaagaaga
     6601 attatccagt ctagtttgga tctgtaccag cactcattcg gctacaaaag ttcttattat
     6661 tgatgctgtt caacctggca acatcctaga cagtttcact gtttgcaact ctcatgttct
     6721 gtgcattgca agtgtgccag gtgcacgaga aacagactac cctgcaggag aagatctttc
     6781 agaatctggt caggtagaca aagcatcttt atgtggaagt atgacaagca acagctcagc
     6841 agagacagac agcctgttag gaggcatcac agtggttggt tgttctgcag aaggtgtgac
     6901 gggagctgcc acttccccta gtacaaatgg tgcttctcca gtgatggata aaccaccaga
     6961 aatggaagca gaaaatagtg aggttgatga aaatgttcca acagcagaag aagcaactga
     7021 agctacagaa gggaatgcgg ggtcagctga agacacagtg gacatctccc aaactggcgt
     7081 ctacacagag catgtcttta cagatccttt gggagttcag atcccagaag acctctcccc
     7141 agtgtatcag tcgagcaatg actcagatgc atataaagat caaatatcag tactgccaaa
     7201 tgaacaagac ttggtgagag aagaagccca gaaaatgagt agtcttttac caactatgtg
     7261 gcttggagct caaaatggct gtttgtatgt ccattcatct gtagcccagt ggaggaaatg
     7321 tctccattcc attaaactta aagattcgat tctcagtatt gtacacgtga agggaatcgt
     7381 gttagtagcc ctggctgacg gcacccttgc aatctttcac agaggagtgg atgggcagtg
     7441 ggatttgtca aactatcacc tcttagacct tggacggcct catcattcca tccgttgcat
     7501 gactgtggta catgacaaag tctggtgtgg ctataggaac aaaatctatg tggtgcagcc
     7561 aaaggccatg aaaatagaga aatcttttga tgcacatccc aggaaggaga gccaagtgcg
     7621 acagcttgcg tgggtggggg atggcgtgtg ggtctccatt cgcttggatt ctacgctccg
     7681 tctctatcat gcacacactt atcaacatct acaggatgtg gacattgagc cttatgtaag
     7741 caaaatgtta ggtactggaa aactgggctt ctcttttgtg agaattacag ctcttatggt
     7801 gtcttgtaat cgtttgtggg tggggacagg aaatggtgtc attatctcca tcccattgac
     7861 agaaaccgta atcctccacc agggacgttt actggggctg agggcaaata aaacctcagg
     7921 tgtaccagga aatcgtcctg gaagtgtaat ccgtgtatat ggtgatgaaa acagtgataa
     7981 agtgactcca gggacattta taccctattg ttcaatggca catgcacagc tttgcttcca
     8041 tgggcaccgg gatgctgtga aattctttgt ggcagtccca ggtcaagtca tcagcccaca
     8101 aagtagcagt agtggcacgg atctgacggg tgacaaagca gggccatctg cacaggagcc
     8161 tggtagtcag acgcccttga agtctatgct tgtcatcagt ggaggagagg gctacatcga
     8221 cttccgaatg ggtgatgaag gtggagaatc agaacttctt ggagaggatc ttccacttga
     8281 accttctgtc accaaagcag aaaggagtca cttgatagtg tggcaagtga tgtatggcaa
     8341 tgagtgagat ccagatctct gcagcatatg tctagaggat cataatcagc cataccacat
     8401 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     8461 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     8521 gcaatagcat cacaaatttc acaaataaag catttttttc actgccgacg tcaggtggca
     8581 cttttcgggg cgatcatatt caataaccct taatataact tcgtataatg tatgctatac
     8641 gaagttatta ggtctgaaga ggagtttacg tccagccaag cttaggatct cgacctcgaa
     8701 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagctgagg cggaaagaac
     8761 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     8821 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     8881 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     8941 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     9001 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     9061 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     9121 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     9181 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     9241 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     9301 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     9361 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     9421 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     9481 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     9541 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     9601 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     9661 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     9721 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     9781 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     9841 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     9901 cttctatcgc cttcttgacg agttcttctg agcgtcgatg atatcagatc cccgggatgc
     9961 agaaattgat gatctattaa acaataaaga tgtccactaa aatggaagtt tttcctgtca
    10021 tactttgtta agaagggtga gaacagagta cctacatttt gaatggaagg attggagcta
    10081 cgggggtggg ggtggggtgg gattagataa atgcctgctc tttactgaag gctctttact
    10141 attgctttat gataatgttt catagttgga tatcataatt taaacaagca aaaccaaatt
    10201 aagggccagc tcattcctcc cactcatgat ctatagatct atagatctct cgtgggatca
    10261 ttgtttttct cttgattccc actttgtggt tctaagtact gtggtttcca aatgtgtcag
    10321 tttcatagcc tgaagaacga gatcagcagc ctctgttcca catacacttc attctcagta
    10381 ttgttttgcc aagttctaat tccatcagaa gctggtcgag atccggaacc cttaatataa
    10441 cttcgtataa tgtatgctat acgaagttat taggtccctc gaagaggttc actatgaggg
    10501 agctgcggtg gaggaggtgg agagaaggcc gcacccttct gggcaggggg aggggagtgc
    10561 cgcaatacct ttatgggagt tctctgctgc ctcccgtctt gtaaggaccg ccctgggcct
    10621 ggaagaagcc ctccctcctt tcctcctcgc gtgatctcgt catcgcctcc atgtcgagtc
    10681 gcttctcgat tatgggcggg attcttttgc ctaggcttaa ggggctaact tggtccctgg
    10741 gcgttgccct gcaggggagt gagcagctgt aagatttgag gggcgactcc gattagttta
    10801 tcttcccacg gactagagtt ggtgtcgagg ttattgtaat aagggtgggg tagggaaatg
    10861 gagcttagtc attcacctgg ggctgatttt atgcaacgag actgcggatt atcactaatc
    10921 atttttggag catttttcta gagacagaca taaagcatga tcacctgagt tttataccat
    10981 ttgagaccct tgctgcacca ccaaagtgta gcatcaggtt aaatcttaat agaaaaattt
    11041 tagcttttgc ttgagaaacc agtgcttccc tccctcaccc tctctcccca ggctctctac
    11101 ccctttgcat ccctaccagg catcttagca actctcactc atacttgatc ccattttcca
    11161 tttgttgtac ttgctcctcc agtattcaga catagcacta gctttctccc tctcttgatc
    11221 ttgggtagcc tggtgtctcg cgaaaccaga cagattggtt ccaccacaaa ttaaggcttg
    11281 agctggggct tgactcttac ccagcagtgc ttttattcct ccctagttca cgttcttaaa
    11341 tgtttatctt gattttcatt ttatcctttt tccttagctg ggattctgtc cctgaccgtc
    11401 ttcacagtcc aggtgatctt gactactgct ttacagagaa ttggatctga ggttaggcaa
    11461 catctccctt tttcttcctc taaatacctc tcatttctgt tcttaccagt tagtaactga
    11521 tctcagatgc ctgtgtgata gctggtaccc agcttttgtt ccctttagtg agggttaatt
    11581 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    11641 attccacaca acatacgagc cgggagcata aagtgtaaag cctggggtgc ctaatgagtg
    11701 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    11761 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    11821 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    11881 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    11941 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    12001 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    12061 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    12121 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    12181 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    12241 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    12301 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    12361 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    12421 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    12481 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    12541 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    12601 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    12661 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    12721 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    12781 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    12841 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    12901 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    12961 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    13021 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    13081 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    13141 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    13201 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    13261 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    13321 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    13381 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    13441 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    13501 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    13561 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    13621 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    13681 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    13741 aaagtgccac
//
