LOCUS       sequence-413184-       12722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12722)
  AUTHORS   Chen LF, Mu Y, Greene WC
  TITLE     Acetylation of RelA at discrete sites regulates distinct nuclear 
            functions of NF-kappaB.
  JOURNAL   EMBO J. 2002 Dec 2. 21(23):6539-48.
  PUBMED    12456660
REFERENCE   2  (bases 1 to 12722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2002 Dec 2. 21(23):6539-48."
COMMENT     Sequence Label: pcDNA3.1-p300
FEATURES             Location/Qualifiers
     source          1..12722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3469..3485)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3469..3485)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3537..3556)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3538..3556)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3601..3804)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3630..3650)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3805..4184
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4356..4375
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4450..4554
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4555..4623,4624..5415)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4773..4792)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5586..6174
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6075..6094
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6328..6345
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6462..6483
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6498..6503,6504..6521,6522..6528)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6536..6552
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6541..6563
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6560..6576
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6560..6576
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6625..6746
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6636..6655)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6690..6709
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6920..7714)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7031..7050)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7641..7660
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7781..8110)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7795..7930
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7849..7868)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8095..8115
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(8124..8552)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8234..8255)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8446..8465
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    8598..8822
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8811..8828
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(8865..8882)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(11130..12722,1..258))
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      11357..11366
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccggttatat aaccaggcat tattgaacat aagccaaata tcatcgacat actgccaggg
       61 ctcctgatac tgtccagtgt ctaacttcct cttaatggta gaaagatcca tggggctctt
      121 cacaatatca aagtaatcag ggattcctaa aagctgaggg tccacaggtt gacgaaaggg
      181 aagggattct ggatcctgac ggtaaagtgc ctccaaagtt ggcatcagtg cctgtcgtag
      241 ttcttctggt ttgaaaatct ttttctttga ctgtcctgga gccggagatg actgggtagc
      301 tgaagtactt ggctggtctt cctcctcttt tatttcagtt tttaactcag tgcttctctc
      361 ttctgtttcg gtagattcca ttttacagtc ttccacttta gactctgaaa tatcctccgg
      421 ctgagtatct gctggttctg gttgatccac ttccattttg gcctccatct tcacttcctg
      481 ggaaggctgc ttctcagcaa tggcctgaga attcacttct gtgctactag tagatggagg
      541 atttgatacc tgtccttcaa tgcttacagc tggctgggaa agtggagttg caggctgcgg
      601 aggaagcagc ggtgctgttg gggtaggaac agacgctgct gtgctctgct gtgagcgagg
      661 ctgctgctgg ggctggtcag cagaaggtgc agcaggaagt gaaggctgca cttgttgggg
      721 aagttgtgtt gttggtggtg taggtgtctg ccttggaggg ggatgtagag cctgggactg
      781 tggcccaggt ggcatggcag gaggtgtagg aacaggggct ggaattgttg ttgctggtgg
      841 ctgctgagcc cctatgcttg ggggagtatg gtgaggggtg ggggtacgac taggtacagg
      901 cgagggtgaa ttctgatgaa gagctggttg aggaagctgg ggacagtgaa tgtggctccc
      961 ctgagaccct ggaggcatta taggagagtt caccgggcag gaagaactag acatttgtgc
     1021 ttgagacact ggagcttgac cgctggacgg agccaaaggg atatttgtta cattcattcc
     1081 ctgtgatggg aactgagtct gaggaaggaa ctggccctgg ttggaaggct gttgcatacg
     1141 aggcccatag cccataggcg ggttgagagc tccaggttga gccaactgtc catggtgctg
     1201 aagaggaggg gtttgccggg gtacaatagg gggctgggcc atgctcatct ggccaaattg
     1261 attcaaacca gattgtggag ttattcgagg catcatctgg tttggcacac tgccacggat
     1321 catacttggg tcaggtagag ggccattaga agtcattcct ggttgcggct gtcccatgtt
     1381 aggccctgga ttcatggaaa ctggaaccat gcctgcagca tttggtagca tgttctgctt
     1441 ctgtagtctg gtccttcgtt tttcttctag ttctttctgg atcttataga ttttctcagc
     1501 tagaaggtgg tagtattccg ctcgattgtt tgcagattca tacatgtccc cttcaacttt
     1561 ccgagcatat gcaactaggt tttccatccg tctgtctttt aaagcagcag gatccggcgt
     1621 aggaaatatg gcttggacga gtttgtgaac aagatgattt cgaagatcct gagtaatatc
     1681 ttcgtgccac tgtttccgaa ttccagtagt ggatggttga gctgctgttg gcataggacc
     1741 catggagggc acactggcat tttcactcat cattgggttt tgagaattta tggctgaatg
     1801 caacattgag tcagaaagaa gactcggcgt ttgaactcct acacctccat ttactcccat
     1861 aggactagca ctcatgttgc tcatgggccg catgccttgg ggagactgcc caggctgctg
     1921 attctgctgg ttctttgctt gcacctgggg ttgtgtcggc atctgattta cttgataggg
     1981 tagtccaaga gctgcatagg ctctttctat ggagctggga tcaatctgac taacagtgct
     2041 taggttgggg gcagactgtt gacccacccc tagagagcta ggatttccaa gtccaacggg
     2101 tgctccagtc aaaattggct gttgatttct cttatcacca gcatttttga gggggagaca
     2161 cacaggacaa tcatgtcttg tacaattctt ccagtgtgaa atgatttgtc gagaagatgc
     2221 acagtgtgcc acttggcaag acttgcctga ctggcagtgt gtcatgtggt ttaggacatt
     2281 cttcattgtg cgacagtggg gaaggttgca ctgcctcact tccccattgg cctgttcccg
     2341 gcgctggcac ttgtgagcat gcaaaaggag aacaagctgc tgctggatga gcttgcgctt
     2401 ctctggatca gctgtatgtg ctccagaacc catcccttgg gcaactggag tcaccagacc
     2461 tggctgctgg acctgcgggg ctggctgttg acccatgttg ggcattcctc caccaggaac
     2521 tgcctttttg tccatagcaa atggagataa gttatttgat agtacagttt ttgtctgaat
     2581 ctggagacca aggccactgg ctccaatctg ctgtccagga ttctgagtat atggtgaacc
     2641 ataaggattg gggttgttca tcattcccat cttaagaggc tgggggcctc tcaatcctgt
     2701 ttgtcctccc atctggggag agccctgctg aagaggctca gtcagtaagt tgccagcact
     2761 tcccatgcct gggtttgggt actgcatatt ctgtcgccct cggcctgctc caattgaacc
     2821 gttcatgact tgattaggca ttatcccttg tccattgcct gcagccaaca ttccaggatt
     2881 catgcccgca ttcatccctg tgttcattcc catggcaggc tgatttactg gactgttcat
     2941 catacctgtt gactgcgtag gaccctgatt tggtccacta gtgcccatcc ccatgttggg
     3001 agaagtcaag cctgcctgtg tcattgggct tttgaccatg ctatttatca aacctaatcc
     3061 aggactgctc tgttgggcct ggctggccat gacttgacct gggccaccaa ctcccatatt
     3121 gaggttaggg gaactaccag atcgcagcaa ttctgacagc tgtttatgtt tagaagctgc
     3181 atcttgtacc atgccaagac ttgtctgaag ctgattaata tcaccaccat tggttagtcc
     3241 caattctgta gagttgatta attcatctgg taagtcgtgc tccaagtcaa atagagagcc
     3301 aaaatctgtg ccatcgctgg cggacgccga gagggccgga gatgagagtt taggccgctt
     3361 ggctgaaggc ggccccggtt ccaccacatt ctcggccatt tttaattctt tcggagatac
     3421 aagcgaggaa aaccagaatc ctcaggaaat ctcttcttcg gcccggggga tccactagtt
     3481 ctagagcggc cgctcgagtc tagagggccc gtttaaacgc tagccagctt gggtctccct
     3541 atagtgagtc gtattaattt cgataagcca gtaagcagtg ggttctctag ttagccagag
     3601 agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc
     3661 ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt
     3721 gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt
     3781 gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg
     3841 ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc
     3901 gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg
     3961 ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac
     4021 tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg
     4081 cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga
     4141 ccccgtaatt gattactatt aataactagt caataatcaa tgtcaacgcg tatatctggc
     4201 ccgtacatcg cgaagcagcg caaaacgcct aaccctaagc agattcttca tgcaattgtc
     4261 ggtcaagcct tgccttgttg tagcttaaat tttgctcgcg cactactcag cgacctccaa
     4321 cacacaagca gggagcagat actggcttaa ctatgcggca tcagagcaga ttgtactgag
     4381 agtgcaccat aggggatcgg gagatctccc gatccgtcga cgtcaggtgg cacttttcgg
     4441 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     4501 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     4561 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     4621 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     4681 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     4741 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     4801 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     4861 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     4921 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     4981 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5041 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     5101 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5161 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5221 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5281 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5341 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     5401 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     5461 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     5521 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5581 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5641 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5701 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     5761 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5821 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5881 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5941 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6001 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6061 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6121 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6181 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6241 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     6301 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     6361 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     6421 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     6481 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     6541 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctctagcta
     6601 gaggtcgacg gtatacagac atgataagat acattgatga gtttggacaa accacaacta
     6661 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     6721 ccattataag ctgcaataaa caagttgggg tgggcgaaga actccagcat gagatccccg
     6781 cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag
     6841 aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt
     6901 tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct
     6961 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
     7021 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
     7081 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
     7141 aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg
     7201 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
     7261 gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg
     7321 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
     7381 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
     7441 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
     7501 tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt
     7561 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
     7621 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
     7681 gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt
     7741 gatcagatcc gaaaatggat atacaagctc ccgggagctt tttgcaaaag cctaggcctc
     7801 caaaaaagcc tcctcactac ttctggaata gctcagaggc agaggcggcc tcggcctctg
     7861 cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta
     7921 ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc
     7981 tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat
     8041 tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta
     8101 actgacacac attccacaga attaattcgc gttaaatttt tgttaaatca gctcattttt
     8161 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     8221 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     8281 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     8341 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     8401 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     8461 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     8521 cgccgcgctt aatgcgccgc tacagggcgc gtggggatac cccctagagc cccagctggt
     8581 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
     8641 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
     8701 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
     8761 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
     8821 agtcgaggct gatcagcggt ttaaacttaa gcttagtatc actaatggtg gtgatggtgg
     8881 tgtatgtcta gtgtactctg tgagaggttt gaattcaagt ctgagttatc ggtgctgagt
     8941 cccaggtccg tggcgcttgc accatggagg tttgccatgc ctggattgct agcaagctga
     9001 gaaagcattg aattctggtc cgggctggca aaatgccctt gttccatggg gttggcctgg
     9061 gcagctacca gtccaggatg tggggaactt gtctgtgggg aaacgtggtg tggagaaggc
     9121 tgaggctgca tccttgggga aggactggag tgggggggct gggactgtgg ccgtggagaa
     9181 gggacaggct ggggagagcg cacttgattg gagagagaat tagggatctg ctggccttgt
     9241 aggtgtgggg actgggcctg atttgggagc atatgctgct gggggctcat ggggttgggc
     9301 tgaacagggg accccatctg ttgctgaagg agtcgctgct gataggcctg tagactggca
     9361 cctgcctctg ctcccaaggc ctggggaagc tggcctatct gtcccatatt tccttgttgc
     9421 atctgttgca tgtgatgctg catccgctgc tgctgctgtg gtgggtagcc aactccttgg
     9481 ggttgctgga actggttatg gttggccatt ccagggccta ttcctggccc tgctccctgt
     9541 tgctgctgct gttgcatcat ttgctgtcgt ctcaagatgt ctcggaattg tgaaggcatg
     9601 gtgttgtggt tcatgttcat ctgctgagcc tgggggctca tccctcccat gggtggctgg
     9661 agttgctgct gtggttgctg ctggggcagg ccagccctct gaacgcccgc ctgcattgga
     9721 ttcatgttct gcatggctgg attggagtgg accccctgct gacctggcat ggtaggtggc
     9781 tgtagccctg gctgcccctg gggcatgcca ggctgcccag ggatgggttg tggattagag
     9841 ttggcatact tggcagcccg ctgcttgatg aatgcagcca acagctgggg gttggcgtga
     9901 aggatactaa gcacctgttg ctgctgcagg ggagagctgg gagacctgag agtccgcaaa
     9961 aggttttgta aggcttgttg agacacagtg cctggtttga gtgggctgat acctacctgg
    10021 cccaatcctg gttgtggagc catgttcaaa ggttgaccat gctgggccac tgacatcatg
    10081 gctggccttg gcatcccaga ctgtagttgc tggggctgag gcaatcctcc ttggctccag
    10141 ggtggctgtt gctgcatccc tgtcggtccc atccctggct ccaaatgccc actgggacct
    10201 ctggtcatgg gaggtgggtt catacccatg ggggccatgg gagtcatcgg gggcatctgg
    10261 tgttggattg gcctttgaaa aatttgcacg tgggccatct ggcgctgcgt ctccgctgct
    10321 ctctgaatct gcattgccat ttccactgct gcaggtgggg gccctggaag gggtggctga
    10381 gcagtctgag gaggggttgg aggggtcacc tggcctgctg ccttaccctg ggacacaggg
    10441 ccagcagctt gagtcctggg caagtagggt ggcatgctat tgggaggggt aggctgaggc
    10501 tgagaagtgg gctggggcgt ctgcggggtg gttggctgtt ggccagttgg tgtcgttgga
    10561 gtggcaggag tgggggaagg gaggccctgt tgctgcccaa ccacaccagt ccgctgcatg
    10621 ctggccatcc tcctgcgaag catttgggcc tgctgtagtc ggtgctgcag ctgttgctgc
    10681 cggagcttct gcttgatgtt taggcagaac ggcaccgggc atttgttctc ctggcagtgc
    10741 ttggcatggt agcagcagag ggcaatgagc tgcttgcaga tggggcaccc gccattggtt
    10801 ttccgtttgc aacccttggt atgctgcaca acccgcttca tcttctggca ggatggcagt
    10861 gagcaattgg cattccgaca ctggcaagca tggaccagag actggatgca gcgctggata
    10921 ctcaggcggc gagaatcgcc tgggctctgg gtggctgcag cctgctggtt gttgctctca
    10981 tcatctaagc caaggcctag tttctccatt ttgtggtcat ggtttttagt gttatagcag
    11041 gtgatacaca agtcataatc ctcacagaca gtacagtgcc agcgtgtctc cacatggtgc
    11101 ttgcattcat tgcaggtgta gacaaagcgg tcctggctct gcgtgtgcag ctccaccagc
    11161 atgcacatgg tggaccactg ggctcttcgg agtgaagaga actccaggtg cttgtccctt
    11221 gccagcgtga gaaacgcatc ccgaccatcc atcagatcgc aggggatgag aggatcagga
    11281 tcaacaatgg gaggcaggga gttggcagca gggccagcaa tgaggcggat cacaaagaag
    11341 acctctttat gcttctccat ggtggcatat agtttctgtg agaggtcgtt agatacattg
    11401 ggcatcccgg gtttcttctt gttgccccta ctcaggctgc tcttattttt gctggttttc
    11461 ttattattct tctttttagc atttttgctg tctcccttgg tcacatctgt gctttcattg
    11521 ctggtgtttt cctctcgttt tctctcttct tcctcctgtt ccagttcctt aatgctttct
    11581 tccagaacat tgggccagaa atcaccctcg aaataaggca attcctttgc acttgttaat
    11641 ctatcttcag tagcttgttt aaaaatatcc ttgtagtcat ggacaatacg ctctgataca
    11701 gccttgtcaa gcattttttt gtaccattcc tgcagtcgct tgggcttggg tatcttctgg
    11761 tcaggaggat ggcaatggaa gatataatca tctccctcac ttggtggaca tgcccaaata
    11821 tgccctgttg tgtaacctaa tttcttgaca tattctaaat atccaattag gatttcatga
    11881 tagactgcag tcctcaagca tttaggacgg aagaaatgaa cactatcgag gtaagatatg
    11941 tatactctcc tctggttggg tggagggcag tcagagccat actcttgaac atgcatgcca
    12001 aagaagcaca ggtcaacacc atcaatttct tcaaaggcaa agagggcttt ggttcggtat
    12061 ggaaaggatt ctgccatctc tccactgtcc acaaaccttg ctttcatgcc tggttttact
    12121 tccacggttt tgtcagaagc atgaactact ctaacagtga cctctcctga ctcagggtga
    12181 ttctgtcgcc tcagaaagtc attcacacga ttctctagaa aggtgccaag tctggtagat
    12241 ggcaaccttt tagcagaaaa cttattttct ttcctagttc gtgcactttt ctttaaacag
    12301 ccatcacaga cgaatccagc aggccagatg atctcatggt gaaggacaca gatctgatgc
    12361 atctttcttc cgcactctgt acattcaaca aacagttcag gatccagtgt gtcatttttt
    12421 ctcttggaaa attgttcttt atttattgta gtttgaggct gggaagggtc atcccccaaa
    12481 gaaacgctct ccccttggat ctcattgaaa cacttctcac agaaatgata cctgttctgg
    12541 taactgtaat aagtggcatc acgaggtatt gtgcacaact gtttgccgta gcaacacagt
    12601 gtctgtggag agaactccaa ctttctgcca caacagtatc caaggctttg catcactggg
    12661 tcaatttctt gttcaaagac ctcagagagc ttggagcagt atttgtatac ccgtgatgtt
    12721 tt
//
