LOCUS       sequence-413216-       10812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-NeoR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10812)
  AUTHORS   Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell
            DL, Peet DJ, Whitelaw ML, Bersten DC
  TITLE     dFLASH; dual FLuorescent transcription factor Activity Sensor for 
            Histone integrated live-cell reporting and high-content screening
  JOURNAL   bioRxiv 2023.11.21.568191
REFERENCE   2  (bases 1 to 10812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.11.21.568191"
COMMENT     Sequence Label: pLV-EgI-NeoR
FEATURES             Location/Qualifiers
     source          1..10812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    159..180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(195..200,201..218,219..225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    233..249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     238..260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     257..273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     257..273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     292..312
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        294..312
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        340..566
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             567..747
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    794..919
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1412..1645
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1830..1874
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2136..2253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(2256..2272)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2257..2273)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        2380..3566
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2611..3557
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3514..3534
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3639..3763
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3788..3793,3794..3811,3812..3818)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3872..4531
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3938..3957)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4873..5178
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5057..5076
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5219..5343)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    join(5400..5974,5975..5977,5978..5986)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5567..5584)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5794..5813
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5987..6781
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6041..6060)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6651..6670
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             8021..8254
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8326..8447
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8363..8382)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8417..8436
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8549..8568
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(8833..8851)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8919..9023
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9024..9092,9093..9884)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9242..9261)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10055..10643
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10544..10563
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgataaccct aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg
       61 gttagtctgg atagtatata ctactacccg ggaagcatat gctacccgtt tagggttcac
      121 cggtgatgcc ggccacgatg cgtccggcgt agaggatcta atgtgagtta gctcactcat
      181 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      241 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
      301 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
      361 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
      421 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
      481 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
      541 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
      601 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      661 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      721 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      781 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      841 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      901 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
      961 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1021 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1081 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1141 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1201 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1261 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1321 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1381 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1441 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1501 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1561 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1621 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1681 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1741 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1801 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1861 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     1921 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     1981 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2041 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2101 ccattcgatt agtgaacgga tctcgacggt atcggtttta aaagaaaagg ggggattggg
     2161 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2221 ttacaaaaac aaattacaaa aattcaaaat tttatcgata ccgtcgacct cgacggatcg
     2281 ggagatctcg accgagcttt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2341 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2401 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     2461 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     2521 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     2581 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     2641 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     2701 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     2761 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     2821 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     2881 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     2941 ggccaagatc gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     3001 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     3061 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     3121 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     3181 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     3241 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     3301 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgatcgagct
     3361 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     3421 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     3481 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     3541 tttttcttcc atttcaggtg tcgtgaggaa ttagcttgac caagcttggt accgagctcg
     3601 gatccactag taacggccgc cagtgtgctg gaattatcac aagtttgtac aaaaaagctg
     3661 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     3721 agactacata atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac
     3781 cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc
     3841 gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
     3901 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
     3961 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
     4021 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
     4081 tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
     4141 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
     4201 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
     4261 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg
     4321 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
     4381 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
     4441 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
     4501 gtactgcgat gagtggcagg gcggggcgta aacgcgtgga tccggcttac taaaagccag
     4561 ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta
     4621 tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac
     4681 agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca
     4741 caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg
     4801 aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca
     4861 ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg
     4921 tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg
     4981 gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc
     5041 ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc
     5101 ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
     5161 atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca
     5221 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
     5281 atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt
     5341 ggtgataatt ctgcagatat ccatcacact ggcggccgct cgagttaatt aagctagcgc
     5401 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     5461 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     5521 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     5581 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     5641 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     5701 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     5761 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     5821 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtac
     5881 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     5941 gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatga ttgaacaaga
     6001 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     6061 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     6121 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     6181 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     6241 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     6301 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     6361 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     6421 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     6481 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     6541 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     6601 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     6661 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     6721 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     6781 agcgggactc tagcctagtg ccatttgttc agtggttcgt agggctttcc cccactgttt
     6841 ggctttcagt tatatggatg atgtggtatt gggggccaag tctgtacagc atcttgagtc
     6901 cctttttacc gctgttacca attttctttt gtctttgggt atacatttaa accctaacaa
     6961 aacaaagaga tggggttact ctctaaattt tatgggttat gtcattggat gttatgggtc
     7021 cttgccacaa gaacacatca tacaaaaaat caaagaatgt tttagaaaac ttcctattaa
     7081 caggcctatt gattggaaag tatgtcaacg aattgtgggt cttttgggtt ttgctgcccc
     7141 ttttacacaa tgtggttatc ctgcgttgat gcctttgtat gcatgtattc aatctaagca
     7201 ggctttcact ttctcgccaa cttacaaggc ctttctgtgt aaacaatacc tgaaccttta
     7261 ccccgttgcc cggcaacggc caggtctgtg ccaagtgttt gctgacgcaa cccccactgg
     7321 ctggggcttg gtcatgggcc atcagcgcat gcgtggaacc ttttcggctc ctctgccgat
     7381 ccatactgcg gaactcctag ccgcttgttt tgctcgcagc aggtctggag caaacattat
     7441 cgggactgat aactctgttg tcctatcccg caaatataca tcgtttccat ggctgctagg
     7501 ctgtgctgcc aactggatcc tgcgcgggac gtcctttgtt tacgtcccgt cggcgctgaa
     7561 tcctgcggac gacccttctc ggggtcgctt gggactctct cgtccccttc tccgtctgcc
     7621 gttccgaccg accacggggc gcacctctct ttacgcggac tccccgtctg tgccttctca
     7681 tctgccggac cgtgtgcact tcgcttcacc tctgcacgtc gcatggagac caccgtgaac
     7741 gcccaccaaa tattgcccaa ggtcttacat aagaggactc ttggactctc agcaatgtca
     7801 acgaccgacc ttgaggcata cttcaaagac tgtttgttta aagactggga ggagttgggg
     7861 gaggagatta ggttaaaggt ctttgtacta ggaggctgta ggcataaatt ggtctgcgca
     7921 ccagcaccat ggcgcaatca ctagagcggg gtacctttaa gaccaatgac ttacaaggca
     7981 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     8041 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     8101 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8161 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8221 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     8281 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     8341 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     8401 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     8461 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     8521 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc ggatcccttg
     8581 agtggctttc atcctggagc agactttgca gtctgtggac tgcaacacaa cattgccttt
     8641 atgtgtaact cttggctgaa gctcttacac caatgctggg ggacatgtac ctcccagggg
     8701 cccaggaaga ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg tagctaccga
     8761 taagcggacc ctcaagaggg cattagcaat agtgtttata aggccccctt gttaattctt
     8821 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     8881 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     8941 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     9001 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     9061 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     9121 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     9181 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     9241 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
     9301 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     9361 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     9421 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     9481 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     9541 acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa
     9601 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     9661 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     9721 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     9781 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     9841 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     9901 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     9961 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    10021 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    10081 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    10141 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    10201 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    10261 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    10321 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    10381 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    10441 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    10501 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    10561 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    10621 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    10681 tttgctggcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct
    10741 gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc
    10801 agcctcgcgt cg
//
