LOCUS       sequence-41332-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Garcia DM, Baek D, Shin C, Bell GW, Grimson A, Bartel DP
  TITLE     Weak seed-pairing stability and high target-site abundance decrease 
            the proficiency of lsy-6 and other microRNAs.
  JOURNAL   Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 
            10.1038/nsmb.2115.
  PUBMED    21909094
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb"; 
            journalName: "Nat Struct Mol Biol"; date: "2011-09-11- 11"; volume: 
            "18"; issue: "10"; pages: "1139-46"
COMMENT     Sequence Label: pDMG8a
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    357..478
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(368..387)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     422..441
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        611..715
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag atgcacctgc atgaaatggg
       61 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctaggagct
      121 ctataccggt ctcgatatca ctactagtgt tctagattgg aaaatgtgat gtttttctat
      181 aaataaatat tctcacaact ctttttcatg ttttatataa tacaaaatgc acatcaagca
      241 gaaaaatttc aacataaagt ttacaccaga agtgaattta gggatgaaga ggaaccaaat
      301 tacgtaaata caaaagtatc gaaacatgat agatcggccg cttcgagcag acatgataag
      361 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
      421 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
      481 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
      541 aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccaggtg gcacttttcg
      601 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      661 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      721 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      781 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
      841 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttt cgccccgaag
      901 a
//
