LOCUS       sequence-413357-       10830 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP10B mutant E210Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10830)
  AUTHORS   Naito T, Takatsu H, Miyano R, Takada N, Nakayama K, Shin HW
  TITLE     Phospholipid Flippase ATP10A Translocates Phosphatidylcholine and Is
            Involved in Plasma Membrane Dynamics.
  JOURNAL   J Biol Chem. 2015 Jun 12;290(24):15004-17. doi: 
            10.1074/jbc.M115.655191. Epub 2015 May 6.
  PUBMED    25947375
REFERENCE   2  (bases 1 to 10830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.655191"; journalName: "J Biol Chem"; date: 
            "2015-06-12- 12"; volume: "290"; issue: "24"; pages: "15004-17"
COMMENT     Sequence Label: pMx/ATP10B(E210Q)-HA
FEATURES             Location/Qualifiers
     source          1..10830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1938..1962
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3045..3056)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6406..6430)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6462..6488
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6657..6677)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6662..6991
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6842..6977
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7348..8142
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7402..7421)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8012..8031
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             8335..8804
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8934..8951)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9105..9693)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9185..9204)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9857..10648,10649..10717))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10480..10499
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10718..10822)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 ctctagttaa gctatcaaca agtttgtaca aaaaagcagg ctctttaaag gaaccaattc
     1981 agtcgactgg atccggtacc gaattcacca tggccctctc agtggactca tcgtggcatc
     2041 ggtggcagtg gagagtcaga gatggcttcc cccattgtcc atcggaaacc acaccgctgc
     2101 tctctccaga gaaagggaga cagagctaca acttgacaca gcagcgggtc gtgttcccca
     2161 acaacagcat attccatcaa gattgggaag aggtctccag gagataccct ggcaacagaa
     2221 cctgcacaac caaatacacc ctcttcacct tcctgccccg gaatctcttt gagcaatttc
     2281 atagatgggc taacctctat ttcctgttcc tggtgatttt gaactggatg ccctccatgg
     2341 aagtcttcca cagagaaatc accatgttac cattggccat tgtcctgttc gtcatcatga
     2401 tcaaggatgg catggaggac ttcaagagac accgctttga taaagcaata aactgctcca
     2461 acattcgaat ttatgaaaga aaagagcaga cctatgtgca gaagtgctgg aaggatgtgc
     2521 gtgtgggaga cttcatccaa atgaaatgca atgagattgt cccagcagac atactcctcc
     2581 ttttttcctc tgaccccaat gggatatgcc atctggaaac tgccagcttg gatggacaga
     2641 caaacctcaa gcaaagacgt gtcgtgaagg gcttctcaca gcaggaggta cagttcgaac
     2701 cagagctttt ccacaatacc atcgtgtgtg agaaacccaa caaccacctc aacaaattta
     2761 agggttatat ggagcatcct gaccagacca ggactggctt tggctgtgag agtcttctgc
     2821 ttcgaggctg caccatcaga aacaccgaga tggctgttgg cattgtcatc tatgcaggcc
     2881 atgagacgaa agccatgctg aacaacagtg gcccccggta caaacgcagc aagattgagc
     2941 ggcgcatgaa tatagacatc ttcttctgca ttgggatcct catcctcatg tgccttattg
     3001 gagctgtagg tcacagcatc tggaatggga cctttgaaga acaccctccc ttcgatgtgc
     3061 cagatgccaa tggcagcttc cttcccagtg cccttggggg cttctacatg ttcctcacaa
     3121 tgatcatcct gctccaggtg ctgatcccca tctctttgta tgtctccatt gagctggtga
     3181 agctcgggca agtgttcttc ttgagcaatg accttgacct gtatgatgaa gagaccgatt
     3241 tatccattca atgtcgagcc ctcaacatcg cagaggactt gggccagatc cagtacatct
     3301 tctccgataa gacggggacc ctgacagaga acaagatggt gttccgacgt tgcaccatca
     3361 tgggcagcga gtattctcac caagaaaatg ctaagcgact ggagacccca aaggagctgg
     3421 actcagatgg tgaagagtgg acccaatacc aatgcctgtc cttctcggct agatgggccc
     3481 aggatccagc aactatgaga agccaaaaag gtgctcagcc tctgaggagg agccagagtg
     3541 cccgggtgcc catccagggc cactaccggc aaaggtctat ggggcaccgt gaaagctcac
     3601 agcctcctgt ggccttcagc agctccatag aaaaagatgt aactccagat aaaaacctac
     3661 tgaccaaggt tcgagatgct gccctgtggt tggagacctt gtcagacagc agacctgcca
     3721 aggcttccct ctccaccacc tcctccattg ctgatttctt ccttgcctta accatctgca
     3781 actctgtcat ggtgtccaca accaccgagc ccaggcagag ggtcaccatc aaaccctcaa
     3841 gcaaggctct ggggacgtcc ctggagaaga ttcagcagct cttccagaag ttgaagctat
     3901 tgagcctcag ccagtcattc tcatccactg caccctctga cacagacctc ggggagagct
     3961 taggggccaa cgtggccacc acagactcgg atgagagaga tgatgcatct gtgtgcagtg
     4021 gaggtgactc cactgatgac ggtggctaca ggagcagcat gtgggaccag ggcgacatcc
     4081 tggagtctgg gtcaggcact tccttggagg aggcattgga ggccccagcc acagacctgg
     4141 ccaggcctga gttctgttac gaggctgaga gccctgatga ggccgccctg gtgcacgctg
     4201 cccatgccta cagcttcaca ctagtgtccc ggacacctga gcaggtgact gtgcgcctgc
     4261 cccagggcac ctgcctcacc ttcagcctcc tctgcaccct gggctttgac tctgtcagga
     4321 agagaatgtc tgtggttgtg aggcacccac tgactggcga gattgttgtc tacaccaagg
     4381 gtgctgactc ggtcatcatg gacctgctgg aagacccagc ctgcgtacct gacattaata
     4441 tggaaaagaa gctgagaaaa atccgagccc ggacccaaaa gcatctagac ttgtatgcaa
     4501 gagatggcct gcgcacacta tgcattgcca agaaggttgt aagcgaagag gacttccgga
     4561 gatgggccag tttccggcgt gaggctgagg catccctcga caaccgagat gagcttctca
     4621 tggaaactgc acagcatctg gagaatcaac tcaccttact tggagccact gggatcgaag
     4681 accggctgca ggaaggagtt ccagatacga ttgccactct gcgggaggct gggatccagc
     4741 tctgggtcct gactggagat aagcaggaga cagcggtcaa cattgcccat tcctgcagac
     4801 tgttaaatca gaccgacact gtttatacca tcaatacaga gaatcaggag acctgtgaat
     4861 ccatcctcaa ttgtgcattg gaagagctaa agcaatttcg tgaactacag aagccagacc
     4921 gcaagctctt tggattccgc ttaccttcca agacaccatc catcacctca gaagctgtgg
     4981 ttccagaagc tggattggtc atcgatggga agacattgaa tgccatcttc cagggaaagc
     5041 tagagaagaa gtttctggaa ttgacccagt attgtcggtc cgtcctgtgc tgccgctcca
     5101 cgccactcca gaagagtatg atagtcaagc tggtgcgaga caagttgcgc gtcatgaccc
     5161 tttccatagg tgatggagca aatgatgtaa gcatgattca agctgctgat attggaattg
     5221 gaatatctgg acaggaaggc atgcaggctg tcatgtccag cgactttgcc atcacccgct
     5281 ttaagcatct caagaagttg ctgctcgtgc atggccactg gtgttactcg cgcctggcca
     5341 ggatggtggt gtactacctc tacaagaacg tgtgctacgt caacctgctc ttctggtatc
     5401 agttcttctg tggtttctcc agctccacca tgattgatta ctggcagatg atattcttca
     5461 atctcttctt tacctccttg cctcctcttg tctttggagt ccttgacaaa gacatctctg
     5521 cagaaacact cctggcattg cctgagctat acaagagtgg ccagaactct gagtgctata
     5581 acctgtcgac tttctggatt tctatggtgg atgcattcta ccagagcctc atctgtttct
     5641 ttatccctta cctggcctat aagggctctg atatagatgt ctttaccttt gggacaccaa
     5701 tcaacaccat ctccctcacc acaatccttt tgcaccaggc aatggaaatg aagacatgga
     5761 ccattttcca cggagtcgtg ctcctcggca gcttcctgat gtactttctg gtatccctcc
     5821 tgtacaatgc cacctgcgtc atctgcaaca gccccaccaa tccctattgg gtgatggaag
     5881 gccagctctc aaaccccact ttctacctcg tctgctttct cacaccagtt gttgctcttc
     5941 tcccaagata ctttttcctg tctctgcaag gaacttgtgg gaagtctcta atctcaaaag
     6001 ctcagaaaat tgacaaactc cccccagaca aaagaaacct ggaaatccag agttggagaa
     6061 gcagacagag gcctgcccct gtccccgaag tggctcgacc aactcaccac ccagtgtcat
     6121 ctatcacagg acaggacttc agtgccagca ccccaaagag ctctaaccct cccaagagga
     6181 agcatgtgga agagtcagta ctccacgaac agagatgtgg cacggagtgc atgagggatg
     6241 actcatgctc aggggactcc tcagctcaac tctcatccgg ggagcacctg ctgggaccta
     6301 acaggataat ggcctactca agaggacaga ctgatatgtg ccggtgctca aagaggagca
     6361 gccatcgccg atcccagagt tcactgacca tctcgagata tctagaccca gctttcttgt
     6421 acaaagtggt tgatctagag ggcccgcggt tcgaactcga gtatccatac gatgtaccag
     6481 actacgcaga gatccactag ttcaattcca gctgagcgcc ggtcgctacc attaccagtt
     6541 ggtctggtgt caaaaataat aataaccggg caggccatgt ctgcccgtat ttcgcgtaag
     6601 gaaatccatt atgtactatt taaactcgag cggccgccag cacagtggtc gactgtggaa
     6661 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     6721 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     6781 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     6841 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     6901 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     6961 aggctttttt ggaggcctag gcttttgcaa aaagcttcac gctgccgcaa gcactcaggg
     7021 cgcaagggct gctaaaggaa gcggaacacg tagaaagcca gtccgcagaa acggtgctga
     7081 ccccggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga
     7141 aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt tttatggaca
     7201 gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa
     7261 gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc aagatctgat
     7321 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     7381 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     7441 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     7501 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     7561 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     7621 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     7681 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     7741 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     7801 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     7861 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     7921 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     7981 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     8041 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     8101 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     8161 catcgtcgac gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
     8221 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa
     8281 atacataact gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat
     8341 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     8401 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     8461 cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc
     8521 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa
     8581 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga
     8641 gcccacaacc cctcactcgg cgcgccagtc ctccgattga ctgagtcgcc cgggtacccg
     8701 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
     8761 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcacatgca gcatgtatca
     8821 aaattaattt ggtttttttt cttaagtatt tacattaaat ggccatagtt gcattaatga
     8881 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8941 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     9001 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     9061 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9121 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9181 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9241 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     9301 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9361 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9421 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9481 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9541 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9601 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9661 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9721 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9781 aggatcttca cctagatcct tttgcggccg gccgcaaatc aatctaaagt atatatgagt
     9841 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9901 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9961 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    10021 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    10081 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    10141 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    10201 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    10261 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    10321 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    10381 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    10441 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    10501 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10561 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10621 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10681 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10741 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10801 ataaacaaat aggggttccg cgcacatttc
//
