LOCUS       sequence-413360-        9513 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP11C isoform b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9513)
  AUTHORS   Inoue H, Takatsu H, Hamamoto A, Takayama M, Nakabuchi R, Muranaka Y,
            Yagi T, Nakayama K, Shin HW
  TITLE     The interaction of ATP11C-b with ezrin contributes to its polarized 
            localization.
  JOURNAL   J Cell Sci. 2021 Oct 15;134(20):jcs258523. doi: 10.1242/jcs.258523. 
            Epub 2021 Oct 18.
  PUBMED    34528675
REFERENCE   2  (bases 1 to 9513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-10-15"; volume: "134(20):jcs258523. doi"; pages: 
            " 10.1242/jcs.258523. Epub 2021 Oct 18"
COMMENT     Sequence Label: pCAG/ATP11C-b-HA
FEATURES             Location/Qualifiers
     source          1..9513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             400..1203
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(463..482)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1073..1092
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1210..1258
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        1268..1647
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2865..2887
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2949..2968
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3037..3061
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(6172..6183)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     protein_bind    complement(6467..6491)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6535..6561
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6649..6668)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6714..6769
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6768..6787)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7130..7146)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7130..7146)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7143..7165)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7154..7170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7178..7184,7185..7202,7203..7208))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7223..7244
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7303..7498
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7349..7484
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7504..7638
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7541..7560)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7595..7614
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7706..7723)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7877..8465)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7957..7976)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8636..9427,9428..9496))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9259..9278
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9497..9513,1..88))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
      121 gcagtgtggt tttcaagagg aagcaaaaag cctctccacc caggcctgga atgtttccac
      181 ccaatgtcga gcagtgtggt tttgcaagag gaagcaaaaa gcctctccac ccaggcctgg
      241 aatgtttcca cccaatgtcg agcaaacccc gcccagcgtc ttgtcattgg cgaattaatt
      301 cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat attaaggtga
      361 cgcgtgtggc ctcgaacacc gagcgaccct gcagccaata tgggatcggc cattgaacaa
      421 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
      481 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
      541 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
      601 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
      661 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
      721 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
      781 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
      841 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
      901 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgatgatctc
      961 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     1021 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     1081 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     1141 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     1201 tgaggggatc ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcgg
     1261 atccgtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     1321 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1381 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     1441 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     1501 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1561 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1621 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
     1681 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
     1741 gcagcgatgg gggcgggggg gggggggggg gcgcccccgg gggggggggg gggggggggg
     1801 gggggggggg gggggggggg gggggggggg gggggcgccc aggggggggg gggggggggg
     1861 ggtttttttt tagggggggg gggggggggg ggggccccaa aaaaaaggga gggccggggg
     1921 gggggggggg gcggggcggg gcgtcccccc cggccccccc cccccccccc ccccgccccc
     1981 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     2041 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     2101 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
     2161 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
     2221 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     2281 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     2341 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     2401 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     2461 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     2521 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc
     2581 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     2641 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     2701 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     2761 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     2821 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     2881 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     2941 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aatttgctag
     3001 cgagctcgcg gccgcagatc tctagttaag ctatcaacaa gtttgtacaa aaaagcaggc
     3061 tctttaaagg aaccaattca gtcgactgga tccaccatgc agatggtccc atctctccct
     3121 ccagcctctg agtgtgctgg agaagagaaa cgagttggca cacgcacagt gtttgttggc
     3181 aatcatccag tttcggaaac agaagcttac attgcacaaa gattttgtga taatagaata
     3241 gtctcatcta agtatacact ttggaatttt ctcccaaaga atctgtttga acagtttaga
     3301 agaattgcaa atttttattt tctcataatc ttccttgtac aggtcacagt agacacacca
     3361 actagcccag ttaccagtgg acttccactt ttctttgtta taactgttac agccatcaag
     3421 cagggatatg aggattggct gagacacaga gctgacaatg aagtcaacaa aagcactgtt
     3481 tacattattg aaaatgcaaa gcgagtgaga aaagaaagtg aaaaaatcaa ggttggtgat
     3541 gtagtagaag tacaggcaga tgaaaccttt ccctgtgatc ttattcttct atcatcttgc
     3601 accactgatg gaacctgtta tgtcactaca gccagtcttg atggggaatc caattgcaag
     3661 acacattatg cagtacgtga taccattgca ctgtgtacag cagaatccat cgataccctc
     3721 cgagcagcaa ttgaatgtga acagcctcaa cctgacctct acaaatttgt tgggcgaatc
     3781 aatatctaca gtaatagtct tgaggctgtt gccaggtctt tgggacctga aaatctcttg
     3841 ctgaaaggag ctacgctaaa aaataccgag aagatatatg gagttgctgt ttacactgga
     3901 atggaaacca aaatggcttt gaactaccaa gggaaatctc agaaacgttc tgctgttgaa
     3961 aaatctatta atgctttcct gattgtatat ttatttatct tactgaccaa agctgcagta
     4021 tgcactactc taaagtatgt ttggcaaagt accccataca atgatgaacc ttggtataac
     4081 caaaagactc agaaagagcg agagaccttg aaggttttaa aaatgttcac cgacttccta
     4141 tcatttatgg ttctattcaa ctttatcatt cctgtctcca tgtacgtcac agtagaaatg
     4201 cagaaattct tgggctcctt cttcatctca tgggataagg acttttatga tgaagaaatt
     4261 aatgaaggag ccctggttaa cacatcagac cttaatgaag aacttggtca ggtggattat
     4321 gtatttacag ataagactgg aacactcact gaaaacagca tggaattcat tgaatgctgc
     4381 atagatggcc acaaatataa aggtgtaact caagaggttg atggattatc tcaaactgat
     4441 ggaactttaa catattttga caaagtagat aagaatcgag aagagctgtt tctacgtgcc
     4501 ttgtgtttat gtcatactgt agaaatcaaa acaaacgatg ctgttgatgg agctacagaa
     4561 tcagctgaat taacctatat ctcctcttca ccagatgaaa tagctttggt gaaaggagct
     4621 aaaaggtacg ggttcacatt tttaggaaat cgaaatggat atatgagagt agagaaccaa
     4681 agaaaagaaa tagaagaata tgaacttctt cacaccttaa actttgatgc tgtccggcga
     4741 cgtatgagtg taattgtgaa gactcaagaa ggagacatac ttctcttttg taaaggagca
     4801 gactcggcag tttttcccag agtgcaaaat catgaaattg agttaactaa agtccatgtg
     4861 gaacgtaatg caatggatgg gtatcggaca ctctgtgtag ccttcaaaga aattgctcca
     4921 gatgattatg aaagaattaa cagacagctc atagaggcaa aaatggcctt acaagacaga
     4981 gaagaaaaaa tggaaaaagt tttcgatgat attgagacaa acatgaattt aattggagcc
     5041 actgcagttg aagacaagct acaagatcaa gctgcagaga ccattgaagc tctgcatgca
     5101 gcaggcctga aagtctgggt gctcactggg gacaagatgg agacagctaa atccacatgc
     5161 tatgcctgcc gccttttcca gaccaacact gagctcttag aactaaccac aaaaaccatt
     5221 gaagaaagtg aaaggaaaga agatcgatta catgaattat tgatagaata tcgcaagaaa
     5281 ttgctgcatg agtttcctaa aagtactaga agctttaaaa aagcatggac agaacatcag
     5341 gaatatggat taatcataga tggctccaca ttgtcactca tactaaattc tagtcaagac
     5401 tctagttcaa acaattacaa aagcattttc ctacaaatat gtatgaagtg tactgcagtg
     5461 ctctgctgtc ggatggcacc attacagaaa gcccagattg tcagaatggt gaagaattta
     5521 aaaggcagcc caataactct gtcgataggt gatggtgcca atgatgttag tatgatcttg
     5581 gaatcccatg tgggaatagg tattaaaggc aaagaaggtc gccaagcagc taggaatagc
     5641 gattattctg ttccaaagtt taaacactta aagaaactgc tgttggctca tggacatcta
     5701 tattatgtga gaatagcaca ccttgtacag tacttcttct ataagaacct ttgtttcatt
     5761 ttgccacagt ttttgtacca gttcttctgt ggattctcac aacagccact gtatgatgct
     5821 gcttacctta caatgtacaa tatctgcttc acatccttgc ccatcctggc ctatagtcta
     5881 ctggaacagc acatcaacat tgacactctg acctcagatc cccgattgta tatgaaaatt
     5941 tctggcaatg ccatgctaca gttgggcccc ttcttatatt ggacatttct ggctgccttt
     6001 gaagggacag tgttcttctt tgggacttac tttctttttc agactgcatc cctagaagaa
     6061 aatggaaagg tatacggaaa ctggactttt ggaaccattg tttttacagt cttagtattc
     6121 actgtaaccc tgaagcttgc cttggatacc cgattctgga cgtggataaa tcactttgtg
     6181 atttggggtt ctttagcctt ctatgtattt ttctcattct tctggggagg aattatttgg
     6241 ccttttctca agcaacagag aatgtatttt gtatttgccc aaatgctgtc ttctgtatcc
     6301 acatggttgg ctataattct tctaatattt atcagcctgt tccctgagat tcttctgata
     6361 gtattaaaga atgtaagaag aagaagtgcc aggaatccga atcttgaact gcctatgtta
     6421 ttgtcctaca agcatactga cagtggttac agctcgagat atctagaccc agctttcttg
     6481 tacaaagtgg ttgatctaga gggcccgcgg ttcgaactcg aggtaccgaa ttcgtaccca
     6541 tacgacgtcc cagactacgc ttaattcact cctcaggtgc aggctgccta tcagaaggtg
     6601 gtggctggtg tggccaatgc cctggctcac aaataccact gagatcgatc tttttccctc
     6661 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
     6721 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     6781 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
     6841 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat
     6901 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
     6961 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
     7021 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
     7081 tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt
     7141 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     7201 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     7261 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc
     7321 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7381 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     7441 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     7501 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7561 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7621 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7681 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7741 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7801 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7861 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7921 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7981 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8041 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8101 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8161 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8221 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8281 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8341 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8401 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8461 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8521 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8581 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8641 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8701 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8761 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8821 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8881 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8941 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9001 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9061 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9121 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9181 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9241 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9301 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9361 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9421 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9481 aatgttgaat actcatactc ttcctttttc aat
//
