LOCUS       sequence-41341-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Garcia DM, Baek D, Shin C, Bell GW, Grimson A, Bartel DP
  TITLE     Weak seed-pairing stability and high target-site abundance decrease 
            the proficiency of lsy-6 and other microRNAs.
  JOURNAL   Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 
            10.1038/nsmb.2115.
  PUBMED    21909094
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb"; 
            journalName: "Nat Struct Mol Biol"; date: "2011-09-11- 11"; volume: 
            "18"; issue: "10"; pages: "1139-46"
COMMENT     Sequence Label: pDMG11a
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    265..386
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(276..295)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     330..349
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        519..623
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag atgcacctgc atgaaatggg
       61 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctaggagct
      121 ctataccggt ctcgatatca ctactagtgt tctagattga gcctttatat tgtatttgaa
      181 tgagctttga gtattataat gattatctct cttggaaacg tttttgtaca aaataaacaa
      241 agcggccgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta
      301 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
      361 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
      421 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa
      481 tcgataagga tccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      541 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      601 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
      661 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
      721 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
      781 agatccttga gagttttcgc cccgaagaac gttttcccat gatgagc
//
