LOCUS       sequence-413513-       10011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP8B4 mutant E170Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10011)
  AUTHORS   Takatsu H, Tanaka G, Segawa K, Suzuki J, Nagata S, Nakayama K, Shin 
            HW
  TITLE     Phospholipid flippase activities and substrate specificities of 
            human type IV P-type ATPases localized to the plasma membrane.
  JOURNAL   J Biol Chem. 2014 Nov 28;289(48):33543-56. doi: 
            10.1074/jbc.M114.593012. Epub 2014 Oct 14.
  PUBMED    25315773
REFERENCE   2  (bases 1 to 10011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.593012"; journalName: "J Biol Chem"; date: 
            "2014-11-28- 28"; volume: "289"; issue: "48"; pages: "33543-56"
COMMENT     Sequence Label: pMx/ATP8B4(E170Q)-HA
FEATURES             Location/Qualifiers
     source          1..10011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             15..608
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    671..1028
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1087..1503
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1433..1455
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1471..1487
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1513..1887
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1825..1841
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1931..1955
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5580..5604)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5636..5662
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(5831..5851)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5836..6165
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6016..6151
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6522..7316
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6576..6595)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7186..7205
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             7509..7978
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8108..8125)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8279..8867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8359..8378)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9031..9822,9823..9891))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9654..9673
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9892..9996)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agtgccacct gcataatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg
       61 ccattttgca aggcatggaa aaatacataa ctgagaatag aaaagttcag atcaaggtca
      121 ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
      181 cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
      241 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc
      301 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga
      361 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct
      421 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggcgcgccag tcctccgatt
      481 gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg
      541 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt
      601 ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc
      661 accgggaggt aagctggcca gcaacttatc tgtgtctgtc cgattgtcta gtgtctatga
      721 ctgattttat gcgcctgcgt cggtactagt tagctaacta gctctgtatc tggcggaccc
      781 gtggtggaac tgacgagttc ggaacacccg gccgcaaccc tgggagacgt cccagggact
      841 tcgggggccg tttttgtggc ccgacctgag tccaaaaatc ccgatcgttt tggactcttt
      901 ggtgcacccc cctaatagga gggatatgtg gttctggtag gagacgagaa cctaaaacag
      961 ttcccgcctc cgtctgaatt tttgctttcg gtttgggacc gaagccgcgc cgcgcgtctt
     1021 gtctgctgca gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa
     1081 aattagggcc agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc
     1141 gagcggatcg ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc
     1201 tctgcagaat ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac
     1261 ctcatcaccc aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag
     1321 gtcccctaca tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc
     1381 tttgtacacc ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa
     1441 cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc
     1501 gcccccatat ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct
     1561 gaccctgaca tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc
     1621 tacttagtcc agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac
     1681 cgaccggtgg tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag
     1741 actaagaacc tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc
     1801 gccctcaaag tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac
     1861 cccgggggtg gaccatcctc tagactgccg gatctagcta gttaattaag gatctctagt
     1921 taagctatca acaagtttgt acaaaaaagc aggctcttta aaggaaccaa ttcagtcgac
     1981 tggatccacc atgttctgca gtgaaaagaa attgcgtgaa gtggaacgga tagtgaaagc
     2041 caatgaccgt gaatataatg aaaagttcca gtatgcggat aatcgtatcc acacatcgaa
     2101 atataatatt ctcaccttct tgccaattaa tttatttgaa cagttccaaa gagtggcaaa
     2161 tgcctatttt ctttgccttc tgattttaca gctaattcca gaaatttcct ccttgacctg
     2221 gtttaccacc attgtgcctt tggtcctggt gataactatg acagctgtca aagatgccac
     2281 agatgactat tttcgccaca agagtgataa tcaagtgaat aatcggcagt ctgaagtgct
     2341 catcaacagc aaactgcaga atgaaaaatg gatgaatgtc aaagtgggag acatcattaa
     2401 attagaaaat aaccaatttg ttgctgctga tttacttctc ctatcaagta gtgagccaca
     2461 tggtctctgt tatgttgaaa ctgctgagct tgatgggcaa acgaacctaa aagtccgcca
     2521 tgcactatca gttacttcag aacttggagc agatatcagc agacttgcag ggtttgatgg
     2581 gattgttgtc tgtgaggtgc ctaacaacaa gttagataaa ttcatgggaa tcctttcttg
     2641 gaaagacagc aagcattccc tcaacaatga gaagataatc ctgagaggct gcatcctgag
     2701 aaataccagc tggtgttttg gaatggttat ttttgcaggt cctgacacta aactaatgca
     2761 gaatagtggt aagacaaagt ttaaaaggac aagcattgat agattgatga atactctagt
     2821 actatggatt tttgggtttc tgatatgctt gggaattatt cttgcaatag gaaattcaat
     2881 ctgggagagt caaactgggg accaattcag aactttcctc ttttggaatg aaggagagaa
     2941 gagctctgtg ttctccggat tcttaacatt ctggtcatat attattattc tcaatacagt
     3001 tgtacccatt tccttatatg tgagtgtgga agtaattcgt ctaggacaca gttattttat
     3061 aaactgggac cggaagatgt attattctcg aaaagcaata cctgcagtgg ctcgaacgac
     3121 cacgctcaat gaggaactgg ggcagattga gtacattttc tccgacaaaa cgggtaccct
     3181 cactcaaaac atcatgacct ttaaaagatg ttccattaat gggagaatct atggtgaagt
     3241 acatgatgac ctggatcaga agacagaaat aactcaggaa aaagagcctg tggatttctc
     3301 agtcaaatct caagcggata gagaatttca gttctttgac caccatctga tggaatccat
     3361 taaaatgggt gatcccaaag ttcatgaatt ccttaggtta cttgctctct gccacactgt
     3421 aatgtcagaa gagaatagcg caggagagct gatttaccaa gttcagtcac ctgatgaagg
     3481 ggctctagtg actgccgcta gaaattttgg gttcattttt aaatcccgga ccccagagac
     3541 cataacaata gaagaattgg gaacactagt tacttatcaa ttacttgcct ttttggattt
     3601 caacaacacc agaaaaagga tgtctgtcat agttcgaaac ccagaaggac agataaagct
     3661 ttattccaaa ggagcagata ctattctgtt tgaaaaactt catccttcca atgaagtcct
     3721 tttgtctttg acgtcagacc acctcagtga atttgcaggg gaaggccttc ggaccttggc
     3781 catcgcatac agagacctgg atgacaagta ctttaaagag tggcataaga tgcttgaaga
     3841 tgcgaatgct gccacagaag agagggatga acgaatagct gagctatatg aagaaattga
     3901 aagagatttg atgctactag gtgccactgc tgtagaagat aagttacagg agggtgttat
     3961 tgaaacagtt acaagtttat cactagccaa tattaagatc tgggtcctaa caggagacaa
     4021 acaagaaact gccatcaaca tcggttatgc ctgcaacatg ctgactgacg acatgaatga
     4081 tgtgtttgtg atagcaggga ataatgctgt ggaagtgaga gaagaactca ggaaagcaaa
     4141 acaaaatttg tttggacaaa acagaaattt ttccaatggc catgtagttt gtgaaaaaaa
     4201 gcagcagctg gagttggatt ctattgtaga agaaaccata acaggagatt atgccttaat
     4261 cataaatggc cacagtttgg ctcatgccct agaaagtgat gtcaagaatg atctcctaga
     4321 acttgcttgc atgtgtaaga ctgtaatttg ctgcagggtc actccactcc agaaagccca
     4381 agtggtagag ctggtgaaga agtacagaaa tgctgttact ttggccattg gtgatggagc
     4441 caatgatgtc agcatgatta aaagtgctca cattggtgtt ggcatcagcg gccaggaagg
     4501 attgcaagca gtcttagcca gcgactattc atttgcacag tttagatatc tccaaaggct
     4561 tctccttgtt catggaaggt ggtcttattt ccgaatgtgc aaattcttat gctatttctt
     4621 ctataagaat tttgcattta cacttgtgca tttctggttt ggtttcttct gtggtttctc
     4681 agcccagact gtttatgacc agtggttcat cacccttttt aacattgttt acacatcact
     4741 gcctgtttta gccatgggga tttttgacca ggatgtgagt gaccagaaca gcgtggactg
     4801 tccccagctc tacaaaccag gacagctgaa tctgcttttt aacaagcgta aatttttcat
     4861 ttgcgtgttg catggaatct acacctcatt agtccttttc ttcatcccct atggggcctt
     4921 ttacaacgtg gctggagaag atgggcaaca tattgctgac taccagtcct ttgcagttac
     4981 catggccaca tctttggtca ttgtggtcag tgtgcagata gccttggata ccagttactg
     5041 gactttcatt aatcacgtct tcatctgggg gagcattgcc atttatttct ccattttatt
     5101 tacaatgcac agtaatggca tctttggcat cttcccaaac cagtttccat ttgttggtaa
     5161 tgcacgacat tccctgaccc agaagtgcat ctggcttgta attctcttaa caacagtggc
     5221 ttcagttatg ccagtggtgg cattcagatt tttgaaggtg gatttatacc caaccctgag
     5281 tgatcagatc cgccggtggc agaaggctca aaagaaggca aggcctccaa gtagccgaag
     5341 gcctcggacc cgcaggtcaa gctcaagaag gtctggatat gcttttgctc accaagaagg
     5401 ctatggagag cttatcacat ctggaaaaaa tatgcgagct aaaaatccac ccccaacatc
     5461 agggctggaa aagacacatt ataatagcac tagctggatt gaaaatttat gtaagaaaac
     5521 cacagacacc gtgagcagct ttagccagga taaaacagtg aaactctcga gatatctaga
     5581 cccagctttc ttgtacaaag tggttgatct agagggcccg cggttcgaac tcgagtatcc
     5641 atacgatgta ccagactacg cagagatcca ctagttcaat tccagctgag cgccggtcgc
     5701 taccattacc agttggtctg gtgtcaaaaa taataataac cgggcaggcc atgtctgccc
     5761 gtatttcgcg taaggaaatc cattatgtac tatttaaact cgagcggccg ccagcacagt
     5821 ggtcgactgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     5881 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     5941 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     6001 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6061 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     6121 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tcacgctgcc
     6181 gcaagcactc agggcgcaag ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc
     6241 agaaacggtg ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg
     6301 caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg
     6361 cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg
     6421 ggaagccctg caaagtaaac tggatggctt tcttgccgcc aaggatctga tggcgcaggg
     6481 gatcaagatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     6541 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     6601 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     6661 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     6721 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     6781 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     6841 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     6901 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     6961 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     7021 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     7081 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     7141 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     7201 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     7261 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     7321 gactctgggg ttcgcatcgt cgacgataaa ataaaagatt ttatttagtc tccagaaaaa
     7381 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
     7441 caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga
     7501 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     7561 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
     7621 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
     7681 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
     7741 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
     7801 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
     7861 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
     7921 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcaca
     7981 tgcagcatgt atcaaaatta atttggtttt ttttcttaag tatttacatt aaatggccat
     8041 agttgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8101 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8161 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8221 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8281 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8341 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8401 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8461 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8521 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8581 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8641 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8701 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8761 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8821 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8881 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8941 tgagattatc aaaaaggatc ttcacctaga tccttttgcg gccggccgca aatcaatcta
     9001 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9061 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9121 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9181 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9241 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9301 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9361 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9421 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9481 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9541 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9601 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9661 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9721 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9781 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9841 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9901 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9961 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa a
//
