LOCUS       sequence-413668-       12345 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing human codon-optimized LbCas12a with 
            8xNLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12345)
  AUTHORS   Xiao MS, Damodaran AP, Kumari B, Dickson E, Xing K, On TA, Parab N, 
            King HE, Perez AR, Guiblet WM, Duncan G, Che A, Chari R, Andresson 
            T, Vidigal JA, Weatheritt RJ, Aregger M, Gonatopoulos-Pournatzis T
  TITLE     Genome-scale exon perturbation screens uncover exons critical for 
            cell fitness.
  JOURNAL   Mol Cell. 2024 Jul 11;84(13):2553-2572.e19. doi: 
            10.1016/j.molcel.2024.05.024. Epub 2024 Jun 24.
  PUBMED    38917794
REFERENCE   2  (bases 1 to 12345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.05.024"; journalName: "Mol Cell"; date: 
            "2024-07-11- 11"; volume: "84"; issue: "13"; pages: "2553-2572.e19"
COMMENT     Sequence Label: pLenti-LbCas12a-8xNLS
FEATURES             Location/Qualifiers
     source          1..12345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1486..1506
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    1707..1824
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1873..3050
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2104..3041
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2998..3018
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3066..3086
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3111..6791
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     CDS             6792..6839
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6846..6872
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             6882..6908
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             6918..6944
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             6954..6980
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             6990..7016
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             7026..7052
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             7062..7091
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             7101..7154
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7158..7952
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7212..7231)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7822..7841
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7971..8559
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8024..8044)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8442..8453)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8631..8864
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8942..9063
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8979..8998)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9033..9052
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9103..9238
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9258..9277)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9259..9277)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9287..9304)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9287..9303)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9296..9318)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9445..9900
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9532..9551)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9742..9763
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9926..10030
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10031..10099,10100..10891)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10249..10268)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11062..11650
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11551..11570
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11804..11821
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11938..11959
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11974..11979,11980..11997,11998..12004)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12012..12028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12017..12039
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12036..12052
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12036..12052
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12071..12091
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12073..12091
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        12119..12345
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccagagaggg cctatttccc
     1501 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tagaattaat
     1561 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     1621 gggtagtttg cagttttaaa attatgtttt aaaatggact atcataaatt ctagatcttg
     1681 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     1741 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     1801 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
     1861 cgccggctcg agggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     1921 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
     1981 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     2041 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     2101 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
     2161 gtgccttgaa ttacttccac tggctgcagt acgtgattct tgatcccgag cttcgggttg
     2221 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag
     2281 ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct
     2341 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc
     2401 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg
     2461 tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc
     2521 ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg
     2581 ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc
     2641 ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct
     2701 caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa
     2761 gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca
     2821 ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt
     2881 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc
     2941 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca
     3001 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga gttaacacca
     3061 tggccccaaa gaagaagcgg aaggtcggta tccacggagt cccagcagcc agcaagctgg
     3121 agaagtttac aaactgctac tccctgtcta agaccctgag gttcaaggcc atccctgtgg
     3181 gcaagaccca ggagaacatc gacaataagc ggctgctggt ggaggacgag aagagagccg
     3241 aggattataa gggcgtgaag aagctgctgg atcgctacta tctgtctttt atcaacgacg
     3301 tgctgcacag catcaagctg aagaatctga acaattacat cagcctgttc cggaagaaaa
     3361 ccagaaccga gaaggagaat aaggagctgg agaacctgga gatcaatctg cggaaggaga
     3421 tcgccaaggc cttcaagggc aacgagggct acaagtccct gtttaagaag gatatcatcg
     3481 agacaatcct gccagagttc ctggacgata aggacgagat cgccctggtg aacagcttca
     3541 atggctttac cacagccttc accggcttct ttgataacag agagaatatg ttttccgagg
     3601 aggccaagag cacatccatc gccttcaggt gtatcaacga gaatctgacc cgctacatct
     3661 ctaatatgga catcttcgag aaggtggacg ccatctttga taagcacgag gtgcaggaga
     3721 tcaaggagaa gatcctgaac agcgactatg atgtggagga tttctttgag ggcgagttct
     3781 ttaactttgt gctgacacag gagggcatcg acgtgtataa cgccatcatc ggcggcttcg
     3841 tgaccgagag cggcgagaag atcaagggcc tgaacgagta catcaacctg tataatcaga
     3901 aaaccaagca gaagctgcct aagtttaagc cactgtataa gcaggtgctg agcgatcggg
     3961 agtctctgag cttctacggc gagggctata catccgatga ggaggtgctg gaggtgttta
     4021 gaaacaccct gaacaagaac agcgagatct tcagctccat caagaagctg gagaagctgt
     4081 tcaagaattt tgacgagtac tctagcgccg gcatctttgt gaagaacggc cccgccatca
     4141 gcacaatctc caaggatatc ttcggcgagt ggaacgtgat ccgggacaag tggaatgccg
     4201 agtatgacga tatccacctg aagaagaagg ccgtggtgac cgagaagtac gaggacgatc
     4261 ggagaaagtc cttcaagaag atcggctcct tttctctgga gcagctgcag gagtacgccg
     4321 acgccgatct gtctgtggtg gagaagctga aggagatcat catccagaag gtggatgaga
     4381 tctacaaggt gtatggctcc tctgagaagc tgttcgacgc cgattttgtg ctggagaaga
     4441 gcctgaagaa gaacgacgcc gtggtggcca tcatgaagga cctgctggat tctgtgaaga
     4501 gcttcgagaa ttacatcaag gccttctttg gcgagggcaa ggagacaaac agggacgagt
     4561 ccttctatgg cgattttgtg ctggcctacg acatcctgct gaaggtggac cacatctacg
     4621 atgccatccg caattatgtg acccagaagc cctactctaa ggataagttc aagctgtatt
     4681 ttcagaaccc tcagttcatg ggcggctggg acaaggataa ggagacagac tatcgggcca
     4741 ccatcctgag atacggctcc aagtactatc tggccatcat ggataagaag tacgccaagt
     4801 gcctgcagaa gatcgacaag gacgatgtga acggcaatta cgagaagatc aactataagc
     4861 tgctgcccgg ccctaataag atgctgccaa aggtgttctt ttctaagaag tggatggcct
     4921 actataaccc cagcgaggac atccagaaga tctacaagaa tggcacattc aagaagggcg
     4981 atatgtttaa cctgaatgac tgtcacaagc tgatcgactt ctttaaggat agcatctccc
     5041 ggtatccaaa gtggtccaat gcctacgatt tcaacttttc tgagacagag aagtataagg
     5101 acatcgccgg cttttacaga gaggtggagg agcagggcta taaggtgagc ttcgagtctg
     5161 ccagcaagaa ggaggtggat aagctggtgg aggagggcaa gctgtatatg ttccagatct
     5221 ataacaagga cttttccgat aagtctcacg gcacacccaa tctgcacacc atgtacttca
     5281 agctgctgtt tgacgagaac aatcacggac agatcaggct gagcggagga gcagagctgt
     5341 tcatgaggcg cgcctccctg aagaaggagg agctggtggt gcacccagcc aactccccta
     5401 tcgccaacaa gaatccagat aatcccaaga aaaccacaac cctgtcctac gacgtgtata
     5461 aggataagag gttttctgag gaccagtacg agctgcacat cccaatcgcc atcaataagt
     5521 gccccaagaa catcttcaag atcaatacag aggtgcgcgt gctgctgaag cacgacgata
     5581 acccctatgt gatcggcatc gataggggcg agcgcaatct gctgtatatc gtggtggtgg
     5641 acggcaaggg caacatcgtg gagcagtatt ccctgaacga gatcatcaac aacttcaacg
     5701 gcatcaggat caagacagat taccactctc tgctggacaa gaaggagaag gagaggttcg
     5761 aggcccgcca gaactggacc tccatcgaga atatcaagga gctgaaggcc ggctatatct
     5821 ctcaggtggt gcacaagatc tgcgagctgg tggagaagta cgatgccgtg atcgccctgg
     5881 aggacctgaa ctctggcttt aagaatagcc gcgtgaaggt ggagaagcag gtgtatcaga
     5941 agttcgagaa gatgctgatc gataagctga actacatggt ggacaagaag tctaatcctt
     6001 gtgcaacagg cggcgccctg aagggctatc agatcaccaa taagttcgag agctttaagt
     6061 ccatgtctac ccagaacggc ttcatctttt acatccctgc ctggctgaca tccaagatcg
     6121 atccatctac cggctttgtg aacctgctga aaaccaagta taccagcatc gccgattcca
     6181 agaagttcat cagctccttt gacaggatca tgtacgtgcc cgaggaggat ctgttcgagt
     6241 ttgccctgga ctataagaac ttctctcgca cagacgccga ttacatcaag aagtggaagc
     6301 tgtactccta cggcaaccgg atcagaatct tccggaatcc taagaagaac aacgtgttcg
     6361 actgggagga ggtgtgcctg accagcgcct ataaggagct gttcaacaag tacggcatca
     6421 attatcagca gggcgatatc agagccctgc tgtgcgagca gtccgacaag gccttctact
     6481 ctagctttat ggccctgatg agcctgatgc tgcagatgcg gaacagcatc acaggccgca
     6541 ccgacgtgga ttttctgatc agccctgtga agaactccga cggcatcttc tacgatagcc
     6601 ggaactatga ggcccaggag aatgccatcc tgccaaagaa cgccgacgcc aatggcgcct
     6661 ataacatcgc cagaaaggtg ctgtgggcca tcggccagtt caagaaggcc gaggacgaga
     6721 agctggataa ggtgaagatc gccatctcta acaaggagtg gctggagtac gcccagacca
     6781 gcgtgaagca caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg
     6841 gatcccctgc tgctaaacgt gttaagcttg atgggggtag cccggcagcc aagagagtca
     6901 aactcgacgg cggctcccca gcggcaaaaa gggtgaaact agacgggggt agccccgccg
     6961 cgaagcgtgt aaagctggat ggaggatcgc ctgcggctaa gcgagtcaaa ttagacggtg
     7021 ggtcaccggc tgcaaagcgc gtcaaattgg atgctggatc cgaacaaaaa ctcatctcag
     7081 aagaggatct gggcagtgga gagggcagag gaagtctgct aacatgcggt gacgtcgagg
     7141 agaatcctgg cccaaccatg attgaacaag atggattgca cgcaggttct ccggccgctt
     7201 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     7261 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     7321 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     7381 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     7441 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     7501 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     7561 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     7621 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     7681 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     7741 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     7801 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     7861 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     7921 ccttctatcg ccttcttgac gagttcttct gataaacgcg ttaagtcgac aatcaacctc
     7981 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     8041 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     8101 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     8161 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     8221 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     8281 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     8341 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     8401 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     8461 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     8521 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
     8581 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     8641 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     8701 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     8761 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     8821 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc
     8881 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     8941 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     9001 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     9061 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     9121 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     9181 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9241 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     9301 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     9361 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     9421 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     9481 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     9541 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     9601 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     9661 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     9721 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     9781 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     9841 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     9901 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     9961 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
    10021 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
    10081 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
    10141 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
    10201 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
    10261 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
    10321 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
    10381 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
    10441 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
    10501 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
    10561 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
    10621 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
    10681 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
    10741 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
    10801 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
    10861 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
    10921 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    10981 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    11041 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    11101 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    11161 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
    11221 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    11281 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    11341 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    11401 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    11461 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    11521 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    11581 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    11641 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    11701 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    11761 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    11821 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    11881 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    11941 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
    12001 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
    12061 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
    12121 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
    12181 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
    12241 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
    12301 cattgcagag atattgtatt taagtgccta gctcgataca taaac
//
