LOCUS       sequence-413682-       10295 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production of syn-stemar-13-ene in E. coli MEV20.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10295)
  AUTHORS   Lin GM, Voigt CA
  TITLE     Design of a redox-proficient Escherichia coli for screening 
            terpenoids and modifying cytochrome P450s
  JOURNAL   Nature Catalysis. 6, 1016–1029 (2023). 
            https://doi.org/10.1038/s41929-023-01049-5
REFERENCE   2  (bases 1 to 10295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature 
            Catalysis. 6, 1016–1029 (2023). 
            https://doi.org/10.1038/s41929-023-01049-5"
COMMENT     Sequence Label: pLRD16
FEATURES             Location/Qualifiers
     source          1..10295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..86
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(90..133)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    136..157
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        178..196
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    197..221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        1752..1770
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1771..1795
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        4188..4206
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4207..4231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4345..4353
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    6843..6863
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      6864..6921
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(6945..6962)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      7496..7718
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             7766..8716
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFWIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     CDS             complement(join(9330..10121,10122..10190))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9953..9972
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10191..10295)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
       61 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
      121 taaggatgat ttctggaatt cgcggccgct tctagagcgc gaagacttaa tcaaatctaa
      181 tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctacgt tttcaaggtg
      241 gtagactgtc gccggatgtg tatccgacct gacgatggcc caaaagggcc gaaacagtcc
      301 tctacaaata attttgttta agagaatacc ccgctaagaa ggtaattatg tccatgcaaa
      361 gtttagataa gaattttcga catttatctc gtaaagaaaa gttacaacaa ttggttgata
      421 agcaatggtt atcagaagaa caattcgaca ttttactgaa tcatccatta atcgatgaag
      481 aagtagccaa tagtttaatt gaaaatgtca tcgcgcaagg tgcattaccc gttggattat
      541 taccgaatat cattgtggac gataaggcat atgttgtacc tatgatggtg gaagagcctt
      601 cagttgtcgc tgcagctagt tatggtgcaa agctagtgaa tcagactggc ggatttaaaa
      661 cggtatcttc tgaacgtatt atgataggtc aaatcgtctt tgatggcgtt gacgatactg
      721 aaaaattatc agcagacatt aaagctttag aaaagcaaat tcataaaatt gcggatgagg
      781 catatccttc tattaaagcg cgtggtggtg gttaccaacg tatagcgatt gatacatttc
      841 ctgagcaaca gttactatct ttaaaagtat ttgttgatac gaaagatgct atgggcgcta
      901 atatgcttaa tacgatttta gaggccataa ctgcattttt aaaaaatgaa tttccgcaaa
      961 gcgacatttt aatgagtatt ttatccaatc atgcaacagc gtccgttgtt aaagttcaag
     1021 gcgaaattga tgttaaagat ttagcaaggg gcgagagaac tggagaagag gttgccaaac
     1081 gaatggaacg tgcttctgta ttggcccaag tagatattca tcgtgcagca acacataata
     1141 aaggtgttat gaatggcata catgctgttg ttttagcaac aggaaatgat acgcgtggtg
     1201 cagaagcaag tgcgcatgca tacgcgagtc gtgacggaca gtatcgtggt attgctacat
     1261 ggcgttacga tcaagatcgt caacgattga ttggtacaat tgaagtgcct atgacattgg
     1321 caatcgttgg cggtggtaca aaagtattac caattgctaa agcttcatta gagctactaa
     1381 atgtagagtc agcacaagaa ttaggtcatg tagttgctgc cgttggttta gcgcaaaact
     1441 ttgcagcatg tcgcgcgctt gtgtcagaag gtattcaaca aggtcatatg agtttacaat
     1501 ataaatcatt agctatcgtt gtaggggcaa aaggtgatga aattgctaaa gtagctgaag
     1561 ctttgaaaaa agaaccccgt gcaaatacac aagcagcgga acatatttta caagaaatta
     1621 gacaacaata ataacggatg taaacagaga aacgtctaac taaaaaggcc tcccaaatcg
     1681 gggggccttt tttattgata acaaaagcct aatacacctg ttcgtgacga ctacggatgc
     1741 gggcaaggtg ctaatacgac tcactatagg ggaattgtga gcggataaca attcccctct
     1801 aaacgccagc cactaaagcg gtcaacgcat gtgctttgcg ttctgatgag acagtgatgt
     1861 cgaaaccgcc tctacaaata attttgttta agaacctaat tttataagga acacctaatg
     1921 ctgatttcaa aaagcccgcc ttaccctgcg tccgaagaaa cacgggaatg ggaggccgag
     1981 ggccagcatg aacataccga tgaattgcgt gaaacaacca cgacgatgat tgatggcatc
     2041 agaacagccc tgcgcagtat cggggaaggg gaaatctcga ttagcgccta cgatacgtca
     2101 ctggtagcgc tcctgaaacg cctggatggt ggggatgggc cgcagttccc gtcaacgatc
     2161 gattggatcg tacagaatca gttgcccgat ggtagctggg gcgatgcgag cttctttatg
     2221 atgggagatc gcatcatgtc caccctggcg tgcgtagttg ctctcaaatc ttggaacatt
     2281 cataccgaca aatgtgaacg tggtctgctg ttcattcagg agaatatgtg gcggttggct
     2341 cacgaagaag aagattggat gcttgtgggg ttcgaaatcg cgctcccatc gctgctggac
     2401 atggccaagg atttggatct cgatattcca tacgatgagc ctgctctgaa ggctatttac
     2461 gcggaacgcg agcgtaaatt ggcgaaaatc ccacgtgacg tgttacacgc tatgcctacc
     2521 acgctgctgc actccctgga aggcatggtg gatctggatt gggaaaaact tctgaaactg
     2581 cgctgtttgg acggaagttt ccactgcagc cctgcatcga ccgccaccgc gtttcaacaa
     2641 accggcgacc agaaatgctt cgagtacctg gatggtattg tcaagaaatt caatggcggc
     2701 gttccgtgca tctatccgct tgatgtctat gaacgcctgt gggccgttga ccgcctcacc
     2761 cgtctgggta tttcgcgtca ttttacatcc gaaatcgagg attgcctgga ctacattttc
     2821 cgcaactgga cgcccgacgg cctggctcat accaaaaact gcccggtaaa agatatagat
     2881 gataccgcta tgggatttcg acttttacgc ctgtatggat accaggtaga tccctgcgtg
     2941 ctgaagaaat tcgaaaaaga tggcaaattt ttctgccttc atggcgaaag caacccgtca
     3001 tctgtaacac cgatgtataa cacgtaccgc gcaagccaac tgaaatttcc gggtgatgac
     3061 ggggtgttag gtcgtgcaga ggtcttttgt cgcagctttc tccaggatcg ccgaggctcc
     3121 aatcgtatga aagataaatg ggctatcgcg aaagacattc ccggcgaggt tgaatatgcc
     3181 atggattatc cttggaaggc gtccttaccg cgtatagaaa cccgcttgta tctggatcag
     3241 tatggtggta gcggcgatgt ctggattggc aaagttctgc accgtatgac gctgttttgc
     3301 aatgatctgt atttgaaagc ggcgaaggcg gacttctcga attttcagaa agaatgccgc
     3361 gtagagctta atggcctgcg tcgttggtat ctgcgttcca atctcgagcg ttttggcggc
     3421 acggacccgc aaacgaccct gatgacctct tattttcttg cgtccgccaa catattcgag
     3481 ccgaaccgcg ccgccgaacg tcttggttgg gcacgtgtag ccctgctggc cgatgccgtt
     3541 agttcgcact ttcggcgcat cggtgggccg aaaaatctga ccagtaacct tgaggaatta
     3601 atctccctgg ttccctttga tgatgcctat tccggctccc tgcgagaagc gtggaagcag
     3661 tggttgatgg cctggacggc gaaagaatcg tcccaggaaa gtatcgaggg ggataccgcc
     3721 atattgttgg tgcgagcgat tgagatcttt gggggacgcc atgtgctgac tggtcagcgt
     3781 ccggaccttt gggaatattc tcagctggaa caattaacca gcagcatttg ccgcaaactt
     3841 tatagacggg tgctggcaca agaaaatggc aagagcactg agaaagtaga agagattgac
     3901 cagcagttag atctcgagat gcaggagttg acccgccgcg tgctgcaggg ctgcagcgct
     3961 attaaccgtc ttacacgtga gacattcctt catgtggtga agtctttctg ttacgttgca
     4021 tattgcagcc ccgaaaccat cgacaaccac attgacaaag ttatcttcca ggatgtcatc
     4081 taataattat gccttccgct aatcaactcg gtaccaaatt ccagaaaaga gacgctttcg
     4141 agcgtctttt ttcgttttgg tccgtggaca agcacagcat agccaagtaa tacgactcac
     4201 tataggggaa ttgtgagcgg ataacaattc ccctctaggt ccgaagatac ttagggtgtc
     4261 tcaaggtgcg taccttgact gatgagtccg aaaggacgaa acacccctct acaaataatt
     4321 ttgtttaaga ttataactgt tccttaagga ggttttttat ggctggcggc gttcgtaata
     4381 acttagaagt cgttggaaat gcaggtacct tacaaggcat ggatatcgac gaattgcgcg
     4441 tcatcgtgcg caagcagtta cagggtgtcg aattgtctcc ctcgagctat gataccgcct
     4501 gggtcgcaat ggtgccggtt cagggatcga gacaaagtcc ttgttttcct caatgcgtcg
     4561 aatggatttt acagaatcaa caagaggatg gttcctgggg tcatagcgcc ggacctagtg
     4621 gagaggttaa caaggatatc ctgctgagca cacttgcctg cgtactggcc ctcaacattt
     4681 ggaatgtcgg acaagatcac atccgacgtg ggctgagctt tattggccgt aatttttctg
     4741 tagcgattga tggtcaatgt gcggcacctg ttggctttaa cattacgttc tcaggaatgc
     4801 tgcggctggc gattgggatg ggcttaaagt tcccggtgat ggaaaccgac attgactcta
     4861 tctttcgact gcgggaagtc gagtttgaac gtgacgcagg gggcaccgcc agcgcacgaa
     4921 aagcgtttat ggcgtatgtg agcgaagggc tcggccgcga acaagactgg gatcatgtca
     4981 tggcatacca gcggaaaaat ggctcgctgt tcaacagccc tagcacgacc gccgccagcg
     5041 caatccactc ttgtaatgat cgcgccctgg actatctggt gagtctcacc tctaaattgg
     5101 gtggccccgt tccggcgatt tatccagaca aagtctactc tcaactgtgc atggtggata
     5161 cactggagaa aatgggcatt tctagtgact ttgcttgtga catccgcgac attctggaca
     5221 tgacctacag ctgctggatg caggatgaag aagaaatcat gctggatatg gcgacctgtg
     5281 ccaaggcctt tcgtctgctc cgcatgcatg gctatgatgt tagtagcgag ggtatggcca
     5341 gattcgcgga acgttcttca ttcgatgact ctatacacgc ttatctgaac gataccaagc
     5401 cacttttgga gctgtataaa tcctctcagg tacattttct ggaggaagat tttatcctgg
     5461 aaaacatagg ctcctggtca gccaaactgc tcaagcagca gctgtccttc aataaaattt
     5521 ccaaatccct gatgccggaa gtcgaatatg cgcttaaata cccgttctat gccacagtcg
     5581 aagtcttgga acacaagggc aacatcgaac gttttaatgt gaatggcttt cagcgcctca
     5641 agtctggata ctgcggctcg ggtgcggata aagagatcct cgctctggcg gtcaataagt
     5701 ttcactacgc tcagagcgtg taccaacagg aactgcgcta cctggagagc tgggtcgcag
     5761 aatttcgtct ggacgaactt aaattcgcgc gtgtgattcc gttgcagagc ctgctttcag
     5821 ccgttgtccc gctttttccg tgcgaactgt ctgatgcgag aatcgcatgg tcacaaaacg
     5881 cgattctcac agccgtcgtc gacgatcttt tcgatggagg cgggagcatg gaagagatgt
     5941 taaatctggt cgcactgttt gacaaatggg acgaccacgg cgagataggg ttttgtagca
     6001 gcaatgtaga aattatgttc aacgcggttt acaatacgac caaaagaata ggcgcgaagg
     6061 ctgcgttagt tcaaaaacgt tgtgtgattg atcacatagc agagcagtgg caagtcatgg
     6121 tacgcgccat gctgacggag gcagagtggg ctgccggcaa acatatccca gcgaccatgg
     6181 gcgagtatat gtcagtagca gaaccatcat ttgcactggg accgatcgtc ccggtgtccg
     6241 cgtacctgtt gggcgaagaa ctgccggagg aagcagtgcg tagcccagag tacggacgcc
     6301 tgttaggcct ggctagtgcg gtgggacgct tactcaacga tgtgatgacc tatgaaaaag
     6361 aaatggggac gggcaagctg aatagtgtag tgctgctgca gccacttgcg gcaggtggcg
     6421 cagccagccg tggcggcgga ggtgcgccgg cgccagcgcc ggcctccgtt gaagccgcgc
     6481 gtgctgaagt ccgccgcgcg attcaagcaa gctggcgtga tctgcatggt ttggtattcg
     6541 gaagtggtgg gggttcctcc agcagcatta tcccgcgccc gtgccgggaa gtcttttggc
     6601 acacgggaaa agttgcgagc gtattttacc aggagggcga tggctatgcg cgtaaagcga
     6661 tgcggagcat ggcgaatgct gtcatcttag agccactgca tctccaggaa taataagcct
     6721 cgttgctctt cataaatcat ccctatgacc ccttcaaaaa aaaaaaacac cctaacgggt
     6781 gttttttttt ttttggtctg cctctcgctg acggtcacaa tgcgcttacg aaagtcttcc
     6841 tgtactagta gcggccgctg cagtccggca aaaaagggca aggtgtcacc accctgccct
     6901 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
     6961 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatgaca
     7021 gtaagacggg taagcctgtt gatgataccg ctgccttact gggtgcatta gccagtctga
     7081 atgacctgtc acgggataat ccgaagtggt cagactggaa aatcagaggg caggaactgc
     7141 tgaacagcaa aaagtcagat agcaccacat agcagacccg ccataaaacg ccctgagaag
     7201 cccgtgacgg gcttttcttg tattatgggt agtttccttg catgaatcca taaaaggcgc
     7261 ctgtagtgcc atttaccccc attcactgcc agagccgtga gcgcagcgaa ctgaatgtca
     7321 cgaaaaagac agcgactcag gtgcctgatg gtcggagaca aaaggaatat tcagcgattt
     7381 gcccgagctt gcgagggtgc tacttaagcc tttagggttt taaggtctgt tttgtagagg
     7441 agcaaacagc gtttgcgaca tccttttgta atactgcgga actgactaaa gtagtgagtt
     7501 atacacaggg ctgggatcta ttctttttat ctttttttat tctttcttta ttctataaat
     7561 tataaccact tgaatataaa caaaaaaaac acacaaaggt ctagcggaat ttacagaggg
     7621 tctagcagaa tttacaagtt ttccagcaaa ggtctagcag aatttacaga tacccacaac
     7681 tcaaaggaaa aggactagta attatcattg actagcccat ctcaattggt atagtgatta
     7741 aaatcaccta gaccaattga gatgtatgtc tgaattagtt gttttcaaag caaatgaact
     7801 agcgattagt cgctatgact taacggagca tgaaaccaag ctaattttat gctgtgtggc
     7861 actactcaac cccacgattg aaaaccctac aaggaaagaa cggacggtat cgttcactta
     7921 taaccaatac gctcagatga tgaacatcag tagggaaaat gcttatggtg tattagctaa
     7981 agcaaccaga gagctgatga cgagaactgt ggaaatcagg aatcctttgg ttaaaggctt
     8041 ttggattttc cagtggacaa actatgccaa gttctcaagc gaaaaattag aattagtttt
     8101 tagtgaagag atattgcctt atcttttcca gttaaaaaaa ttcataaaat ataatctgga
     8161 acatgttaag tcttttgaaa acaaatactc tatgaggatt tatgagtggt tattaaaaga
     8221 actaacacaa aagaaaactc acaaggcaaa tatagagatt agccttgatg aatttaagtt
     8281 catgttaatg cttgaaaata actaccatga gtttaaaagg cttaaccaat gggttttgaa
     8341 accaataagt aaagatttaa acacttacag caatatgaaa ttggtggttg ataagcgagg
     8401 ccgcccgact gatacgttga ttttccaagt tgaactagat agacaaatgg atctcgtaac
     8461 cgaacttgag aacaaccaga taaaaatgaa tggtgacaaa ataccaacaa ccattacatc
     8521 agattcctac ctacataacg gactaagaaa aacactacac gatgctttaa ctgcaaaaat
     8581 tcagctcacc agttttgagg caaaattttt gagtgacatg caaagtaagt atgatctcaa
     8641 tggttcgttc tcatggctca cgcaaaaaca acgaaccaca ctagagaaca tactggctaa
     8701 atacggaagg atctgaggtt cttatggctc ttgtatctat cagtgaagca tcaagactaa
     8761 caaacaaaag tagaacaact gttcaccgtt acatatcaaa gggaaaactg tccatatgca
     8821 cagatgaaaa cggtgtaaaa aagatagata catcagagct tttacgagtt tttggtgcat
     8881 tcaaagctgt tcaccatgaa cagatcgaca atgtaacaga tgaacagcat gtaacaccta
     8941 atagaacagg tgaaaccagt aaaacaaagc aactagaaca tgaaattgaa cacctgagac
     9001 aacttgttac agctcaacag tcacacatag acagcctgaa acaggcgatg ctgcttatcg
     9061 aatcaaagct gccgacaaca cgggagccag tgacgcctcc cgtggggaaa aaatcatggc
     9121 aattctggaa gaaatagcgc tttcagccgg caaaccggct gaagccggat ctgcgattct
     9181 gataacaaac tagcaacacc agaacagccc gtttgcgggc agcaaaaccc gtaccgatta
     9241 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9301 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9361 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     9421 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agaaccacgc
     9481 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     9541 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     9601 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     9661 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9721 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9781 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9841 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9901 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9961 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10021 ctctcaagga tcttaccgct gttgagatcc agttcgatat aacccactcg tgcacccaac
    10081 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10141 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    10201 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    10261 tgtatttaga aaaataaaca aataggggtt ccgcg
//
