LOCUS       sequence-413684-       10213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production of syn-stemod-13(17)-ene in E. coli MEV20.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10213)
  AUTHORS   Lin GM, Voigt CA
  TITLE     Design of a redox-proficient Escherichia coli for screening 
            terpenoids and modifying cytochrome P450s
  JOURNAL   Nature Catalysis. 6, 1016–1029 (2023). 
            https://doi.org/10.1038/s41929-023-01049-5
REFERENCE   2  (bases 1 to 10213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature 
            Catalysis. 6, 1016–1029 (2023). 
            https://doi.org/10.1038/s41929-023-01049-5"
COMMENT     Sequence Label: pLRD17
FEATURES             Location/Qualifiers
     source          1..10213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..86
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(90..133)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    136..157
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        178..196
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    197..221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        1752..1770
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1771..1795
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        4188..4206
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4207..4231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(5291..5302)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      6107..6116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6761..6781
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      6782..6839
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(6863..6880)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      7414..7636
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             7684..8634
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFWIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     CDS             complement(join(9248..10039,10040..10108))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9871..9890
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10109..10213)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
       61 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
      121 taaggatgat ttctggaatt cgcggccgct tctagagcgc gaagacttaa tcaaatctaa
      181 tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctacgt tttcaaggtg
      241 gtagactgtc gccggatgtg tatccgacct gacgatggcc caaaagggcc gaaacagtcc
      301 tctacaaata attttgttta agagaatacc ccgctaagaa ggtaattatg tccatgcaaa
      361 gtttagataa gaattttcga catttatctc gtaaagaaaa gttacaacaa ttggttgata
      421 agcaatggtt atcagaagaa caattcgaca ttttactgaa tcatccatta atcgatgaag
      481 aagtagccaa tagtttaatt gaaaatgtca tcgcgcaagg tgcattaccc gttggattat
      541 taccgaatat cattgtggac gataaggcat atgttgtacc tatgatggtg gaagagcctt
      601 cagttgtcgc tgcagctagt tatggtgcaa agctagtgaa tcagactggc ggatttaaaa
      661 cggtatcttc tgaacgtatt atgataggtc aaatcgtctt tgatggcgtt gacgatactg
      721 aaaaattatc agcagacatt aaagctttag aaaagcaaat tcataaaatt gcggatgagg
      781 catatccttc tattaaagcg cgtggtggtg gttaccaacg tatagcgatt gatacatttc
      841 ctgagcaaca gttactatct ttaaaagtat ttgttgatac gaaagatgct atgggcgcta
      901 atatgcttaa tacgatttta gaggccataa ctgcattttt aaaaaatgaa tttccgcaaa
      961 gcgacatttt aatgagtatt ttatccaatc atgcaacagc gtccgttgtt aaagttcaag
     1021 gcgaaattga tgttaaagat ttagcaaggg gcgagagaac tggagaagag gttgccaaac
     1081 gaatggaacg tgcttctgta ttggcccaag tagatattca tcgtgcagca acacataata
     1141 aaggtgttat gaatggcata catgctgttg ttttagcaac aggaaatgat acgcgtggtg
     1201 cagaagcaag tgcgcatgca tacgcgagtc gtgacggaca gtatcgtggt attgctacat
     1261 ggcgttacga tcaagatcgt caacgattga ttggtacaat tgaagtgcct atgacattgg
     1321 caatcgttgg cggtggtaca aaagtattac caattgctaa agcttcatta gagctactaa
     1381 atgtagagtc agcacaagaa ttaggtcatg tagttgctgc cgttggttta gcgcaaaact
     1441 ttgcagcatg tcgcgcgctt gtgtcagaag gtattcaaca aggtcatatg agtttacaat
     1501 ataaatcatt agctatcgtt gtaggggcaa aaggtgatga aattgctaaa gtagctgaag
     1561 ctttgaaaaa agaaccccgt gcaaatacac aagcagcgga acatatttta caagaaatta
     1621 gacaacaata ataacggatg taaacagaga aacgtctaac taaaaaggcc tcccaaatcg
     1681 gggggccttt tttattgata acaaaagcct aatacacctg ttcgtgacga ctacggatgc
     1741 gggcaaggtg ctaatacgac tcactatagg ggaattgtga gcggataaca attcccctct
     1801 aaacgccagc cactaaagcg gtcaacgcat gtgctttgcg ttctgatgag acagtgatgt
     1861 cgaaaccgcc tctacaaata attttgttta agaacctaat tttataagga acacctaatg
     1921 ctgatttcaa aaagcccgcc ttaccctgcg tccgaagaaa cacgggaatg ggaggccgag
     1981 ggccagcatg aacataccga tgaattgcgt gaaacaacca cgacgatgat tgatggcatc
     2041 agaacagccc tgcgcagtat cggggaaggg gaaatctcga ttagcgccta cgatacgtca
     2101 ctggtagcgc tcctgaaacg cctggatggt ggggatgggc cgcagttccc gtcaacgatc
     2161 gattggatcg tacagaatca gttgcccgat ggtagctggg gcgatgcgag cttctttatg
     2221 atgggagatc gcatcatgtc caccctggcg tgcgtagttg ctctcaaatc ttggaacatt
     2281 cataccgaca aatgtgaacg tggtctgctg ttcattcagg agaatatgtg gcggttggct
     2341 cacgaagaag aagattggat gcttgtgggg ttcgaaatcg cgctcccatc gctgctggac
     2401 atggccaagg atttggatct cgatattcca tacgatgagc ctgctctgaa ggctatttac
     2461 gcggaacgcg agcgtaaatt ggcgaaaatc ccacgtgacg tgttacacgc tatgcctacc
     2521 acgctgctgc actccctgga aggcatggtg gatctggatt gggaaaaact tctgaaactg
     2581 cgctgtttgg acggaagttt ccactgcagc cctgcatcga ccgccaccgc gtttcaacaa
     2641 accggcgacc agaaatgctt cgagtacctg gatggtattg tcaagaaatt caatggcggc
     2701 gttccgtgca tctatccgct tgatgtctat gaacgcctgt gggccgttga ccgcctcacc
     2761 cgtctgggta tttcgcgtca ttttacatcc gaaatcgagg attgcctgga ctacattttc
     2821 cgcaactgga cgcccgacgg cctggctcat accaaaaact gcccggtaaa agatatagat
     2881 gataccgcta tgggatttcg acttttacgc ctgtatggat accaggtaga tccctgcgtg
     2941 ctgaagaaat tcgaaaaaga tggcaaattt ttctgccttc atggcgaaag caacccgtca
     3001 tctgtaacac cgatgtataa cacgtaccgc gcaagccaac tgaaatttcc gggtgatgac
     3061 ggggtgttag gtcgtgcaga ggtcttttgt cgcagctttc tccaggatcg ccgaggctcc
     3121 aatcgtatga aagataaatg ggctatcgcg aaagacattc ccggcgaggt tgaatatgcc
     3181 atggattatc cttggaaggc gtccttaccg cgtatagaaa cccgcttgta tctggatcag
     3241 tatggtggta gcggcgatgt ctggattggc aaagttctgc accgtatgac gctgttttgc
     3301 aatgatctgt atttgaaagc ggcgaaggcg gacttctcga attttcagaa agaatgccgc
     3361 gtagagctta atggcctgcg tcgttggtat ctgcgttcca atctcgagcg ttttggcggc
     3421 acggacccgc aaacgaccct gatgacctct tattttcttg cgtccgccaa catattcgag
     3481 ccgaaccgcg ccgccgaacg tcttggttgg gcacgtgtag ccctgctggc cgatgccgtt
     3541 agttcgcact ttcggcgcat cggtgggccg aaaaatctga ccagtaacct tgaggaatta
     3601 atctccctgg ttccctttga tgatgcctat tccggctccc tgcgagaagc gtggaagcag
     3661 tggttgatgg cctggacggc gaaagaatcg tcccaggaaa gtatcgaggg ggataccgcc
     3721 atattgttgg tgcgagcgat tgagatcttt gggggacgcc atgtgctgac tggtcagcgt
     3781 ccggaccttt gggaatattc tcagctggaa caattaacca gcagcatttg ccgcaaactt
     3841 tatagacggg tgctggcaca agaaaatggc aagagcactg agaaagtaga agagattgac
     3901 cagcagttag atctcgagat gcaggagttg acccgccgcg tgctgcaggg ctgcagcgct
     3961 attaaccgtc ttacacgtga gacattcctt catgtggtga agtctttctg ttacgttgca
     4021 tattgcagcc ccgaaaccat cgacaaccac attgacaaag ttatcttcca ggatgtcatc
     4081 taataattat gccttccgct aatcaactcg gtaccaaatt ccagaaaaga gacgctttcg
     4141 agcgtctttt ttcgttttgg tccgtggaca agcacagcat agccaagtaa tacgactcac
     4201 tataggggaa ttgtgagcgg ataacaattc ccctctaggt ccgaagatac ttagggtgtc
     4261 tcaaggtgcg taccttgact gatgagtccg aaaggacgaa acacccctct acaaataatt
     4321 ttgtttaaga aatctaaact ccataagggc gtatataatg atagatgaac tgcgcgtaat
     4381 cgtacgtaaa cagctgcagg gtgtcgaact cagcccgagc agttacgaca cggcctgggt
     4441 cgctatggta ccagtacagg gctcaccgca gtccccgtgt ttcccgcagt gtgtggaatg
     4501 gattctgcaa aatcagcaag aagatgggag ctggggacac tctgccggcc catctggcga
     4561 agttaacaaa gatatcttac tttcaaccct ggcatgtgtg cttgcgttga acacctggaa
     4621 cgtgggccaa gatcacattc gccggggact gagtttcatt ggtcgtaatt tctctgtcgc
     4681 tattgatgga cagtgcgctg cacccgtggg ttttaatatc accttttctg gcatgctgca
     4741 cttggctatt ggtatgggtc tgaaatttcc agtgatggaa accgacatcg acagtatttt
     4801 tcgtttacgc gaggtggaat tcgagcgcga cgcgggagga accgcatccg cacgcaaggc
     4861 gttcatggct tacgtgtcag aaggtctggg acgtgagcaa gattgggatc atgtgatggc
     4921 gtatcagcgc aaaaacggct cattatttaa ttccccgtcg accacagctg cttctgcaat
     4981 tcatagctgc aatgatcgcg ctttagacta cctggtcagc ttaaccagca aattaggtgg
     5041 cccagtgcct gcaatacatc cggataaagt ctattcccag ttatgcatgg ttgatacgtt
     5101 ggagaagatg gggatctcat cggatttcgc ctgtgatatc cgagatattc tggacatgac
     5161 ctatagctgt tggatgcaag acgaagaaga aattatgctc gatatggcca cctgtgcaaa
     5221 agcctttcgt ttattacgca tgcacggata cgatgtatca tctgaaggca tggctcgctt
     5281 cgcagaacgt tcttccttcg atgatagcat acacgcttac ttgaacgata ccaaaccgct
     5341 tctcgagctt tataagtcga gtcagctgca tttcttagaa gaggacctca ttctcgagaa
     5401 tattagcagc tggagtgcca aactgctgaa acagcagttg tcgtccaata aaatcatgaa
     5461 aagtctgatg ccagaagttg aatatgccct caaatatccg ttatatagca ccgtggatgc
     5521 actcgaacac cgtgggaaca ttgagcgctt taatgtgaat ggtttccaaa gaccaaaatc
     5581 tgggtactgc ggttcgggtg ccgataaaga gattctggct ttagctgtcg acaaattcca
     5641 ttacaatcag tctgtgtatc aacaggaact gcgctacctg gagagctggg tcgcggaatt
     5701 tggtctggat gagctgaagt tcgcgcgtgt aattccgctg caatcgctgc tgagtgcctt
     5761 agtgccttta tttccagctg aactttccga cgcacgcata gcctttagtc agaattgtat
     5821 gttaacaacg atggtcgatg acttcttcga cggcggtggg tcaatggaag agatggttaa
     5881 ttttgtcgca ctgatcgacg aatgggacaa ccacggagaa attggctttt gtagtaacaa
     5941 tgtggaaatc atgttcaacg ccatctacaa tactacgaaa cgcaactgcg ctaaagcggc
     6001 tttggttcaa aatcgttgcg tcatggatca tattgcgaaa cagtggcaag tgatggtgcg
     6061 cgccatgaaa actgaagcgg aatgggcggc atctcgccat atccctgcca ccatggaaga
     6121 atacatgagc gttggtgaac cgtcgtttgc cctgggcccc attgtacctc tgagcgctta
     6181 cctgctcggt gaagaactgc cggaagaagc tgtgcgcagc ccggagtatg gccagctcct
     6241 gcgtcacgca tcagccgtcg gtcgtctgct caatgatgtg atgacctacg aaaaagaggt
     6301 gctcacttgg accccgaatt ctgtcctgtt acaagctctg gctgcagcac gtggtggcgg
     6361 agaaagtccg actccaccgt cgcctgcatg tgcggaggcc gcaagaggcg aagtgcgccg
     6421 tgccatccaa gcatcatggc gagatctgca ccgtctggtg ttccgtgatg acgatggcag
     6481 tagcattgtg ccccgtgcat gccgtgaact gttttggggc actgccaaag tggcgaacgt
     6541 gttttaccag gaggtcgacg gttacacgcc caaagctatg cgtggtatgg caaacgcggt
     6601 gatcttggac cctcttcatc tgcagcagtg ataagcctcg ttgctcttca taaatcatcc
     6661 ctatgacccc ttcaaaaaaa aaaaacaccc taacgggtgt tttttttttt ttggtctgcc
     6721 tctcgctgac ggtcacaatg cgcttacgaa agtcttcctg tactagtagc ggccgctgca
     6781 gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat
     6841 tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6901 ggcgagcggt atcagctcac tcaaaggcgg taatgacagt aagacgggta agcctgttga
     6961 tgataccgct gccttactgg gtgcattagc cagtctgaat gacctgtcac gggataatcc
     7021 gaagtggtca gactggaaaa tcagagggca ggaactgctg aacagcaaaa agtcagatag
     7081 caccacatag cagacccgcc ataaaacgcc ctgagaagcc cgtgacgggc ttttcttgta
     7141 ttatgggtag tttccttgca tgaatccata aaaggcgcct gtagtgccat ttacccccat
     7201 tcactgccag agccgtgagc gcagcgaact gaatgtcacg aaaaagacag cgactcaggt
     7261 gcctgatggt cggagacaaa aggaatattc agcgatttgc ccgagcttgc gagggtgcta
     7321 cttaagcctt tagggtttta aggtctgttt tgtagaggag caaacagcgt ttgcgacatc
     7381 cttttgtaat actgcggaac tgactaaagt agtgagttat acacagggct gggatctatt
     7441 ctttttatct ttttttattc tttctttatt ctataaatta taaccacttg aatataaaca
     7501 aaaaaaacac acaaaggtct agcggaattt acagagggtc tagcagaatt tacaagtttt
     7561 ccagcaaagg tctagcagaa tttacagata cccacaactc aaaggaaaag gactagtaat
     7621 tatcattgac tagcccatct caattggtat agtgattaaa atcacctaga ccaattgaga
     7681 tgtatgtctg aattagttgt tttcaaagca aatgaactag cgattagtcg ctatgactta
     7741 acggagcatg aaaccaagct aattttatgc tgtgtggcac tactcaaccc cacgattgaa
     7801 aaccctacaa ggaaagaacg gacggtatcg ttcacttata accaatacgc tcagatgatg
     7861 aacatcagta gggaaaatgc ttatggtgta ttagctaaag caaccagaga gctgatgacg
     7921 agaactgtgg aaatcaggaa tcctttggtt aaaggctttt ggattttcca gtggacaaac
     7981 tatgccaagt tctcaagcga aaaattagaa ttagttttta gtgaagagat attgccttat
     8041 cttttccagt taaaaaaatt cataaaatat aatctggaac atgttaagtc ttttgaaaac
     8101 aaatactcta tgaggattta tgagtggtta ttaaaagaac taacacaaaa gaaaactcac
     8161 aaggcaaata tagagattag ccttgatgaa tttaagttca tgttaatgct tgaaaataac
     8221 taccatgagt ttaaaaggct taaccaatgg gttttgaaac caataagtaa agatttaaac
     8281 acttacagca atatgaaatt ggtggttgat aagcgaggcc gcccgactga tacgttgatt
     8341 ttccaagttg aactagatag acaaatggat ctcgtaaccg aacttgagaa caaccagata
     8401 aaaatgaatg gtgacaaaat accaacaacc attacatcag attcctacct acataacgga
     8461 ctaagaaaaa cactacacga tgctttaact gcaaaaattc agctcaccag ttttgaggca
     8521 aaatttttga gtgacatgca aagtaagtat gatctcaatg gttcgttctc atggctcacg
     8581 caaaaacaac gaaccacact agagaacata ctggctaaat acggaaggat ctgaggttct
     8641 tatggctctt gtatctatca gtgaagcatc aagactaaca aacaaaagta gaacaactgt
     8701 tcaccgttac atatcaaagg gaaaactgtc catatgcaca gatgaaaacg gtgtaaaaaa
     8761 gatagataca tcagagcttt tacgagtttt tggtgcattc aaagctgttc accatgaaca
     8821 gatcgacaat gtaacagatg aacagcatgt aacacctaat agaacaggtg aaaccagtaa
     8881 aacaaagcaa ctagaacatg aaattgaaca cctgagacaa cttgttacag ctcaacagtc
     8941 acacatagac agcctgaaac aggcgatgct gcttatcgaa tcaaagctgc cgacaacacg
     9001 ggagccagtg acgcctcccg tggggaaaaa atcatggcaa ttctggaaga aatagcgctt
     9061 tcagccggca aaccggctga agccggatct gcgattctga taacaaacta gcaacaccag
     9121 aacagcccgt ttgcgggcag caaaacccgt accgattatc aaaaaggatc ttcacctaga
     9181 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9241 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9301 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9361 ctggccccag tgctgcaatg ataccgcgag aaccacgctc accggctcca gatttatcag
     9421 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9481 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9541 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9601 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9661 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9721 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9781 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9841 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9901 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9961 tgagatccag ttcgatataa cccactcgtg cacccaactg atcttcagca tcttttactt
    10021 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10081 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10141 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10201 taggggttcc gcg
//
