LOCUS       sequence-413713-        8190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prgef-1 GPI YFP unc-54 3' UTR C.elegans Pan-neural expression of 
            GPI-YFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8190)
  AUTHORS   Meng J, Ahamed T, Yu B, Hung W, Ei Mouridi S, Wang Z, Zhang Y, Wen 
            Q, Boulin T, Gao S, Zhen M
  TITLE     A tonically active master neuron modulates mutually exclusive motor 
            states at two timescales.
  JOURNAL   Sci Adv. 2024 Apr 12;10(15):eadk0002. doi: 10.1126/sciadv.adk0002. 
            Epub 2024 Apr 10.
  PUBMED    38598630
REFERENCE   2  (bases 1 to 8190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-04-12"; volume: "10(15):eadk0002. doi"; pages: " 
            10.1126/sciadv.adk0002. Epub 2024 Apr 10"
COMMENT     Sequence Label: pJH1371
FEATURES             Location/Qualifiers
     source          1..8190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3944..3972)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4720..4740
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(5318..5335)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5318..5334)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5327..5349)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5547..6002
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5634..5653)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5844..5865
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6019..6038)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6138..6160
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6198..6216)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6284..6388
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6389..6457,6458..7249)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6607..6626)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7420..8008
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7909..7928
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8162..8179
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attcgaatgg
      241 ccatgggacg tcgacgcttg catgcctgca gcgtttccga taccccctta tatcagcaca
      301 cattcagtca tcataccaca aaatgttgtg atagtgatag gcaaaactac cgtatttcct
      361 ctattaatct tgcatgcaag actaattttc gattaacccg taggggtgca agactaatag
      421 agactgcaag actattagag gctgaaatac taattttcgt atgctcaata attttggaaa
      481 ttggcctatt ttttgtagaa acttgatacc gtttaaacaa ggaaaaatac acacttttta
      541 atatttcatc aataatttga acgattttgt gattttaagt tcaatttgcc caaaaaaaga
      601 caatttttct gacgttaccg caaagtaatg ctggtcaggc aaaaagagcg gtgcaaaaat
      661 ataagagact gcaatactaa tagaggaaat acggtaattg aattttagtg aaacttgcga
      721 cagttttcct catttttttg ttattccggc atacgagtgt ggcatacgag tgaggtcatc
      781 ttttgtttct tccgtttctt catcgctttt gaaaaaaaat gttgaagaaa acttcgtagc
      841 cgatagccgg ataatgtttg gcacggcgtt ccaacatata tcattggaaa ttttttaaga
      901 tttttgcgaa aaatcacatt cttcacgatg agaacacgtt attgaaggaa tataaatcaa
      961 gaataacata tagttatatt tctctattac tttaacgtta aatatgagca aatttgagca
     1021 ttttgattgc gatgaaaagc agaatcgagt caactgaaat ccgttcaaaa acctaagctc
     1081 ggttcgcccg aaagtcgatt tttccaatag ccgaacagca ctgcttcttt attttccagg
     1141 tatttttggg gtgtaagaat tatatcttct ttgtttgggt atttacgttt ccgaattccc
     1201 tttcttgatc tgtcgctatt tttcagaaac cagaacagtt tatccttttt catgagaacc
     1261 tgaatagctc aaaaacccgt ttaatttctt ttcatcatct catttgaaac tttcctagtc
     1321 ttcttaatga tttccaggct cctcctacat ttgtatctca agatgcatac actttatttc
     1381 cgctccaatt cgaatcatct acttctcttt tttcttcaac tttctacttt tttccattct
     1441 tttctttcaa atgttcgacg tcttcaagtg actgctcttc attctcctca tttttctcga
     1501 atttcattct tgtctctttt ttgctcaaaa atggaaaatg aaagtgacgt gagatttgga
     1561 cggcgggaca cgggggcagt agaagcagca aaaaggagag aaagaggaca caaataagaa
     1621 gaacgaattc aaaaataagc ggagaggagc tatttccgtc aattctacct ccccaatctt
     1681 catcaattcg gctcaattga atgacgtcac agggagataa accgtttgga tgagcgccgt
     1741 ccatcactga cgccatcccg tttgggacaa gaaaagagaa aaaagagcac aaagtttttg
     1801 gtgacggatc ttgtcaatca tatgaaagtt gttctgattg attgtcagtt tttttcctac
     1861 ttttttggat tctatccact tctgaacttt tgacaagttt caaactttct gaatcatttt
     1921 ctatgcattt tcctggaatt ctttttatgt aaaatatgaa atagaatgtt tttgaattca
     1981 agttctgctt ttttcttctt ttctgttctg ttcgggcttg ggtatgcttt ttttaaaaat
     2041 tattttgcac atcgaccaat aagtgcgcaa cttataaaat taatttattt ttgttaattt
     2101 ttgaaatact tgtattgctt taagtgatct gacctcgccc tgagctttcc acgtagttat
     2161 caaatacaaa tcctccaagg gtaacgtacc tatattactg atctttataa taactttatc
     2221 acctgtccag ttccagagga attctgttaa gcttataggc acagaaggag tcatttctgc
     2281 tggtttttaa tgatccaaat ctttatttca agtaaaaaac tgaacacttg cgaataaaac
     2341 tatcagatta accattcacc aaaaatgtgt ttgaatctaa aactttctca gtattccaaa
     2401 tatagaaata aataaccacg acattgctaa aatctgtctg aattgtgtac tccttaccgt
     2461 gaagtaataa tggatataat gaatcgtttg aaatgaatga tcagcacatt tttggtgaaa
     2521 gatcacaaat aaggaataag cgtcggaaaa taaaacgatt atttcggatc aaaaatttgt
     2581 tgaagatcat atacactcga gaccaagatt attctagaca attttcaaat tggcttcttt
     2641 gtttgcaaat cttcataata atctgtgaag tttggaaatt tgaattttta atcttttttc
     2701 atagattata gtttttattt ctttgcaaaa ctatattaaa aaccgatgca ttgttttagg
     2761 gaaattaatg agccttttgt tcaacactaa aaacaataaa attaaaattt tggcttcatc
     2821 atttgacctt ttttaagttc gaaaactttt ctcgtatttc tgaaccgcca attttttcac
     2881 acatctctag acttttggtg cccgttccag aaagttaagt aattgctatt ctaagaaagt
     2941 tctcaacatt gttttttagt tctgattgaa ttctgatgtt ccaggaatat attttaaatt
     3001 taatattctt tgcactattt ctatactaac taaataataa atagtctaag tatgttcaat
     3061 gagaacaaaa aagctctatc tatatttttc tatcctattt catttatatc cttttcattt
     3121 tgaactcacc agcttctctt cttcttcttg tccatatact tcttaaaagt ctcgaacttc
     3181 tctctctcac tctccatcaa tttctttatg gctaccgctt gcgctctgcc gcttccgaag
     3241 aaagaaggcg ttggcagagc tctcaattct attttttctc gccgtaggct atcgatttct
     3301 cagcttctct tctctcttcc tcactccacc accaccactt cgggcttctt cttttcttca
     3361 ttttcgtctt cttcttcatc ctcttctttt tttttcagtt ccccctcact cctcccctta
     3421 tatgcgcgtg cgagaggggt gcaaaagcag cgcgcatccg agaattgaaa cgagaaaacg
     3481 gagacgcagc agcagttcgt cctcagaaaa atagccagta aaaagagaaa aagatagaga
     3541 gagacctatc gcattttatt ttcaaattgc aattcctgca attcatgtgt gcgtgtgccc
     3601 attttcaatt cttcccgtta tttttcagac cattaccaac gttctttcta gatcttgaga
     3661 caatcttcct tctgctcaat cgttcgtcgt gaagacggca tcgacgacga cgacgatcag
     3721 cagtaaagga tcccccgggc tgcaggaatt cgatatgcag aagctgatct cagaggagga
     3781 cctgcttcag aagcttatct cagaggagga ccttcgacct cgaggggggg cccggtaccg
     3841 gtggataatg aaaacaatca tcgcccttgg gtttctctcc ctagtcgtcg cacaagaccc
     3901 gcctggcatc gatatgagta aaggagaaga acttttcact ggagttgtcc caattcttgt
     3961 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
     4021 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
     4081 atgggtaagt ttaaacatat atatactaac taaccctgat tatttaaatt ttcagccaac
     4141 acttgtcact actttcggtt atggtcttca atgcttcgcc agatacccag atcatatgaa
     4201 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
     4261 tttcaaagat gacgggaact acaagacacg taagtttaaa cagttcggta ctaactaacc
     4321 atacatattt aaattttcag gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
     4381 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
     4441 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
     4501 agttgtaagt ttaaacatga ttttactaac taactaatct gatttaaatt ttcagaactt
     4561 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
     4621 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
     4681 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
     4741 agctgctggg attacacatg gcatggacga actatacaag cttccaagca gcggtcattc
     4801 tagacacaga tatgcactta tacccatacc attagcagta attacaacat gtattgtgct
     4861 gtatatgaat gttctttaag ctagccggcc atacaagtaa tccggatgat cgacgccaac
     4921 gtcgttgaat tttcaaattt taaatactga atatttgttt tttttcctat tatttattta
     4981 ttctctttgt gttttttttc ttgctttcta aaaaattaat tcaatccaaa tctaaacatt
     5041 tttttttctc tttccgtctc ccaattcgta ttccgctcct ctcatctgaa cacaatgtgc
     5101 aagtttattt atcttctcgc tttcatttca ttaggacgtg gggggaattg gtggaagggg
     5161 gaaacacaca aaaggatgat ggaaatgaaa taaggacaca caatatgcaa caacattcaa
     5221 ttcagaaata tggaggaagg tttaaaagaa aacataaaaa tatatagagg aggaaggaaa
     5281 actagtggcc acgtgggccg tgcaccttaa gcttggcact ggccgtcgtt ttacaacgtc
     5341 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     5401 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     5461 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     5521 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     5581 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5641 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5701 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5761 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5821 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5881 tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5941 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     6001 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     6061 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     6121 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     6181 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     6241 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6301 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     6361 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6421 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6481 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6541 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6601 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     6661 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6721 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6781 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6841 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6901 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6961 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     7021 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     7081 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     7141 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     7201 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     7261 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7321 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7381 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7441 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7501 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7561 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7621 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7681 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7741 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7801 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7861 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7921 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7981 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     8041 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     8101 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     8161 cagcgagtca gtgagcgagg aagcggaaga
//
