LOCUS       sequence-414094-        9923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral vector for expressing human B3GNT5 with C-terminal 
            myc-DDK tag, includes nucleotide change in PAM targeting sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9923)
  TITLE     Fucosylated glycoproteins and fucosylated glycolipids play opposing 
            roles in cholera intoxication
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: lentiMutB3GNT5-blast
FEATURES             Location/Qualifiers
     source          1..9923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2..590
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(55..75)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(473..484)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(593..609)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(594..610)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1115..1295
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1321..1338)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1327..1551
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1597..2025
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1684..1703)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1894..1915
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2034..2054)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2039..2368
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2219..2354
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2281..2300
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        2416..2463
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2482..2856
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    2986..3107
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3023..3042)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3077..3096
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3156..3172)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3156..3172)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3169..3191)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3180..3196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3204..3210,3211..3228,3229..3234))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3249..3270
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3387..3404)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3558..4146)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3638..3657)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4317..5108,5109..5177))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4940..4959
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5178..5282)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5357..5376)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5548..5927
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5929..6127
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6082..6102
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             6145..6325
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6372..6497
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6990..7223
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7408..7452
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7750..7867
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7960..8171
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             9364..9393
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9412..9435
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             9445..9501
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9502..9900
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
       61 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
      121 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
      181 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
      241 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
      301 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
      361 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
      421 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
      481 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
      541 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
      601 tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
      661 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
      721 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
      781 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
      841 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
      901 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
      961 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     1021 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     1081 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     1141 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     1201 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     1261 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
     1321 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     1381 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     1441 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     1501 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     1561 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     1621 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     1681 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     1741 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     1801 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     1861 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     1921 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     1981 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     2041 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     2101 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     2161 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     2221 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     2281 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     2341 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     2401 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     2461 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     2521 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     2581 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     2641 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     2701 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     2761 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     2821 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     2881 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     2941 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     3001 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     3061 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     3121 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     3181 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     3241 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     3301 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3361 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3421 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3481 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3541 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3601 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3661 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3721 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     3781 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3841 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3901 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3961 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4021 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4081 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4141 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4201 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4261 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4321 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4381 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4441 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     4501 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4561 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4621 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4681 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4741 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4801 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4861 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4921 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     4981 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5041 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5101 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5161 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5221 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5281 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     5341 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     5401 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     5461 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     5521 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
     5581 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     5641 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     5701 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     5761 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     5821 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     5881 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     5941 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     6001 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     6061 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     6121 taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     6181 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     6241 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     6301 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     6361 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     6421 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     6481 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     6541 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     6601 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     6661 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     6721 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     6781 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     6841 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     6901 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     6961 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     7021 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     7081 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     7141 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     7201 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     7261 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     7321 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     7381 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     7441 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     7501 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     7561 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     7621 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     7681 attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt
     7741 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     7801 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     7861 aaattttcgg gtttattaca gggacagcag agatccagtt tggttaatta gctagctagg
     7921 tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     7981 cagtccccga gaagttgggg ggaggggtcg gcaattgatc cggtgcctag agaaggtggc
     8041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     8101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     8161 ccagaacaca ggaccggttc tagagcgagg agatctgccg ccgcgatcgc catgagaatg
     8221 ttggttagtg gcagaagagt caaaaaatgg cagttaatta ttcagttatt tgctacttgt
     8281 tttttagcga gcctcatgtt tttttgggaa ccaatcgata atcacattgt gagccatatg
     8341 aagtcatatt cttacagata cctcataaat agctatgact ttgtgaatga taccctgtct
     8401 cttaagcaca cctcagcggg gcctcgctac caatacttga ttaaccacaa ggaaaagtgt
     8461 caagctcaag acgtcctcct tttactgttt gtaaaaactg ctcctgaaaa ctatgatcga
     8521 cgttccggaa ttagaaggac gtggggcaat gaaaattatg ttcggtctca gctgaatgcc
     8581 aacatcaaaa ctctgtttgc cttaggaact cctaatccac tggagggaga agaactacaa
     8641 agaaaactgg cttgggaaga tcaaaggtac aatgatataa ttcagcaaga ctttgttgat
     8701 tctttctaca atcttactct gaaattactt atgcagttca gttgggcaaa tacctattgt
     8761 ccacatgcca aatttcttat gactgctgat gatgacatat ttattcacat gccaaatctg
     8821 attgagtacc ttcaaagttt agaacaaatt ggtgttcaag acttttggat tggtcgtgtt
     8881 catcgtgctg cccctcccat tagagataaa agcagcaaat actacgtgtc ctatgaaatg
     8941 taccagtggc cagcttaccc tgactacaca gccggagctg cctatgtaat ctccggtgat
     9001 gtagctgcca aagtctatga ggcatcacag acactaaatt caagtcttta catagacgat
     9061 gtgttcatgg gcctctgtgc caataaaata gggatagtac cgcaggacca tgtgtttttt
     9121 tctggagagg gtaaaactcc ttatcatccc tgcatctatg aaaaaatgat gacatctcat
     9181 ggacacttag aagatctcca ggacctttgg aagaatgcta cagatcctaa agtaaaaacc
     9241 atttccaaag gtttttttgg tcaaatatac tgcagattaa tgaagataat tctcctttgt
     9301 aaaattagct atgtggacac atacccttgt agggctgcgt ttatcacgcg tacgcggccg
     9361 ctcgagcaga aactcatctc agaagaggat ctggcagcaa atgatatcct ggattacaag
     9421 gatgacgacg ataagggatc cggcgcaaca aacttctctc tgctgaaaca agccggagat
     9481 gtcgaagaga atcctggacc gatggccaag cctttgtctc aagaagaatc caccctcatt
     9541 gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc
     9601 gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg
     9661 ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg
     9721 acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc
     9781 cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga
     9841 cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa
     9901 gaattcgata tcaagcttat cgg
//
