LOCUS       sequence-414757-        8412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pMVP Gateway destination vector (3rd generation lentiviral vector) 
            containing 6 consensus PGR binding sites upstream of a minimal 
            2YBTATA promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Aarts MT, Wagner M, van der Wal T, van Boxtel AL, van Amerongen R
  TITLE     A molecular toolbox to study progesterone receptor signaling.
  JOURNAL   J Mammary Gland Biol Neoplasia. 2023 Nov 29;28(1):24. doi: 
            10.1007/s10911-023-09550-0.
  PUBMED    38019315
REFERENCE   2  (bases 1 to 8412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mammary 
            Gland Biol Neoplasia."; date: "2023-11-29"; volume: "28(1):24. doi";
            pages: " 10.1007/s10911-023-09550-0"
COMMENT     Sequence Label: Lenti-6XPRE-GFP
FEATURES             Location/Qualifiers
     source          1..8412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1981..2005
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2249..2269)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             join(2272..2274,2275..2277,2278..2991)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2272..2274,2275..2277,2278..2988)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2317..2338)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2578..2597)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2925..2946
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    2993..3013
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(3190..3209)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     protein_bind    complement(3611..3635)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3687..4003
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3854..3989
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4052..4846
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4106..4125)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4716..4735
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             4933..5166
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5238..5359
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5275..5294)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5329..5348
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5399..5534
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5554..5573)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5555..5573)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5583..5600)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5583..5599)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5592..5614)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5741..6196
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5828..5847)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6038..6059
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6222..6326
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6327..6395,6396..7187)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6545..6564)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7358..7946
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7847..7866
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8100..8117
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8234..8255
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8270..8275,8276..8293,8294..8300)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8308..8324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8313..8335
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8332..8348
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8332..8348
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8367..8387
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8369..8387
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgtctaga ggatccacta gtccagtgtg gtggaattct gcagatatca
     1981 acaagtttgt acaaaaaagc aggctggaga cccaagcttg gtaccgagct cggatctgta
     2041 gctagaacat cctgtacagt atccgtagct agaacatcct gtacaggatc tgtagctaga
     2101 acatcctgta cagtatccgt agctagaaca tcctgtacag gatctgtagc tagaacatcc
     2161 tgtacagtat ccgtagctag aacatcctgt acaggatcct ctagagggta tataatgggg
     2221 gccactcgag catgcatcta gaggcaccca acttttctat acaaagttgc catggtgagc
     2281 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     2341 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     2401 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     2461 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     2521 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     2581 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     2641 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     2701 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     2761 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     2821 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     2881 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     2941 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aacaactttg
     3001 tataataaag ttgtacagtg cctctcctgg ccttggaagt tgccactcca gtgcccacca
     3061 gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata
     3121 ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg
     3181 cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa
     3241 tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc
     3301 aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca
     3361 tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca
     3421 aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg attttaaaat
     3481 aactatacca gcaggaggac gtccagacac agcataggct acctggccat gcccaaccgg
     3541 tgggacattt gagttgcttg cttggcactg tcctctcatg cgttgggtcc actcagtaga
     3601 tgcctgttga acccagcttt cttgtacaaa gtggttgata tccagcacag tggcggccgc
     3661 tcgagtctag aggtggcgat ggttccggtg tggaaagtcc ccaggctccc cagcaggcag
     3721 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     3781 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     3841 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     3901 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     3961 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     4021 tatatccatt ttcggatctg atcagcaccg catgattgaa caagatggat tgcacgcagg
     4081 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     4141 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     4201 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     4261 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     4321 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     4381 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     4441 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     4501 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     4561 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     4621 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     4681 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     4741 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     4801 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctaagcac aattcgagct
     4861 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     4921 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     4981 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     5041 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5101 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     5161 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     5221 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5281 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5341 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     5401 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5461 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     5521 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     5581 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5641 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5701 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     5761 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5821 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5881 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5941 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6001 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6061 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6121 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     6181 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6241 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6301 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6361 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6421 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6481 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6541 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6601 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6661 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6721 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6781 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6841 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6901 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6961 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     7021 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     7081 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7141 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7201 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7261 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7321 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7381 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7441 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7501 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7561 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7621 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7681 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7741 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7801 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7861 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7921 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7981 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8041 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8101 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     8161 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     8221 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     8281 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     8341 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     8401 agctgcaagc tt
//
