LOCUS       sequence-41477-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Schnutgen F, Ehrmann F, Poser I, Hubner NC, Hansen J, Floss T, 
            Devries I, Wurst W, Hyman A, Mann M, von Melchner H
  TITLE     Resources for proteomics in mouse embryonic stem cells.
  JOURNAL   Nat Methods. 2011 Feb . 8(2):103-4.
  PUBMED    21278719
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2011 Feb . 8(2):103-4."
COMMENT     Sequence Label: pDTnLAPP2A frame 0
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             144..200
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(507..526)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
       61 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
      121 gtccgagggc aaaggaacgc ggagccacga acttctctct gttaaagcaa gcaggagatg
      181 ttgaagaaaa ccccgggccc atggtgtcca agggcgagga actgttcacc ggcgtggtgc
      241 ccatcctggt ggagctggac ggcgacgtga acggccacaa gttcagcgtg tccggcgagg
      301 gcgaaggcga cgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      361 tgcccgtgcc ctggcccacc ctggtgacca ccctgaccta cggcgtgcag tgcttcagca
      421 gataccccga ccacatgaag cagcacgatt tcttcaagag cgccatgccc gagggctacg
      481 tccaggagcg caccatcttt ttcaaggacg acggcaacta caagaccagg gccgaggtga
      541 agttcgaggg cgacacactg gtgaacagga tcgagctgaa gggcatcgac ttcaaagagg
      601 acggcaacat cctgggccac aagctggaat acaactacaa cagccacaac gtgtacatta
      661 tggccgacaa gcagaagaac ggcatcaagg tcaacttcaa gatccggcac aacatcgagg
      721 acggcagcgt gcagctggcc gaccactacc agcagaacac ccccatcggc gacggccccg
      781 tgctgctgcc cgacaaccac tacctgagca cccagagcgc tctcagcaag gaccccaacg
      841 agaagcggga ccacatggtg ctgaaagaat ttgtga
//
