LOCUS       sequence-41478-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Schnutgen F, Ehrmann F, Poser I, Hubner NC, Hansen J, Floss T, 
            Devries I, Wurst W, Hyman A, Mann M, von Melchner H
  TITLE     Resources for proteomics in mouse embryonic stem cells.
  JOURNAL   Nat Methods. 2011 Feb . 8(2):103-4.
  PUBMED    21278719
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2011 Feb . 8(2):103-4."
COMMENT     Sequence Label: pDTnLAPP2A frame 0
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    237..270
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    289..358
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             complement(575..598)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(614..634)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(647..691)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(731..754)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
       61 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      121 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
      181 aatactcata ctcttccttt ttcaatggcc ggatccgttg ctgaagttcc tattccgaag
      241 ttcctattct tcaaatagta taggaacttc gttatgcagc gtggaccaga tgggtgaggt
      301 ggagtacgcg cccggggagc ccaagggcac gccctggcac ccgcaccgcg gcttcgagac
      361 cgtcacgaat gcatgatatc cgcggccacc ctcaaaggca tcaccgcggg ccaggtgaat
      421 atcaaatcct cctcgttttt ggaaactgac aatcttagcg cagaagtcat gcccgctttt
      481 gagagggagt actcacccca acagctggcc ctcgcagaca gcgatgcggt agagagtgag
      541 gacgaacgcg ggccctgggc aggtcgtcgg tcagcttgtc gtcgtcgtcc ttatagtccc
      601 ctccgcctcc gcctccctga aagtacaggt tctcgcctcc tcctccgctg tccatgtgct
      661 gccgctcgaa tttagcagca gctgtttctt tgaacttggc ggcgctgcca gaggggccag
      721 agctgctcag ggggccctgg aacagcactt ccaggctgct gctgccgctg gggccgctgc
      781 tct
//
