LOCUS       sequence-41481-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Subach FV, Subach OM, Gundorov IS, Morozova KS, Piatkevich KD, 
            Cuervo AM, Verkhusha VV
  TITLE     Monomeric fluorescent timers that change color from blue to red 
            report on cellular trafficking.
  JOURNAL   Nat Chem Biol. 2009 Feb;5(2):118-26. Epub 2009 Jan 11.
  PUBMED    19136976
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol."; date: "2009-02"; volume: "5(2)"; pages: "118-26. Epub 2009 
            Jan 11"
COMMENT     Sequence Label: pTRE-Slow-FT
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..718)
                     /codon_start=1
                     /product="blue-to-red fluorescent timer derivative of 
                     mCherry (Subach et al., 2009)"
                     /label=Slow-FT
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHVEGSVNGHEFEIVGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSRAYVKHPADIPDYWKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDVEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(286..305)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     555..573
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctctagatta cttgtacagc tcgtccatgc cgccggtgga gtggcggccc tcggcgcgtt
       61 cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt gatgtccaac ttgatgttga
      121 cgttgtaggc gccgggcagc tgcacgggct tcttggcctt gtaggtggtc ttgacctcaa
      181 cgtcgtagtg gccgccgtcc ttcagcttca gcctctgctt gatctcgccc ttcagggcgc
      241 cgtcctcggg gtacatccgc tcggaggagg cctcccagcc catggtcttc ttctgcatta
      301 cggggccgtc ggaggggaag ttggtgccgc gcagcttcac cttgtagatg aactcgccgt
      361 cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc gtcctcgaag ttcatcacgc
      421 gctcccactt gaagccctcg gggaaggaca gcttccagta gtcggggatg tcggcggggt
      481 gcttcacgta ggccctggag ccgtacatga actgagggga caggatgtcc caggcgaagg
      541 gcagggggcc acccttggtc accttcagct tggcggtctg ggtgccctcg taggggcggc
      601 cctcgccctc gcccacgatc tcgaactcgt ggccgttcac ggagccctcc acgtgcacct
      661 tgaaacgcat gaactccttg atgatggcca tgttatcctc ctcgcccttg ctcaccatga
      721 attcggggcc gcggaggctg gatcggtccc ggtgtcttct atggaggtca aaacagcgtg
      781 gatggcgtct ccaggcgatc tgacggt
//
