LOCUS       sequence-414822-        5543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  molecular monitoring of atypical BCR::ABL1 fusion transcripts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Schafer V, White HE, Gerrard G, Mobius S, Saussele S, Franke GN, 
            Mahon FX, Talmaci R, Colomer D, Soverini S, Machova Polakova K, 
            Cross NCP, Hochhaus A, Ernst T
  TITLE     Assessment of individual molecular response in chronic myeloid 
            leukemia patients with atypical BCR-ABL1 fusion transcripts: 
            recommendations by the EUTOS cooperative network.
  JOURNAL   J Cancer Res Clin Oncol. 2021 Oct;147(10):3081-3089. doi: 
            10.1007/s00432-021-03569-8. Epub 2021 Mar 7.
  PUBMED    33677711
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00432-021-03569-8"; journalName: "J Cancer Res Clin 
            Oncol"; date: "2021-10"; volume: "147"; issue: "10"; pages: 
            "3081-3089"
COMMENT     Sequence Label: pUC18_BCR_GUSB_e8a2
FEATURES             Location/Qualifiers
     source          1..5543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      578..1166
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1067..1086
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1320..1337
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1454..1475
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1490..1495,1496..1513,1514..1520)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1528..1544
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1533..1555
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1552..1568
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1552..1568
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4495..4512)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4495..4511)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4504..4526)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4720..4739)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4839..4861
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4899..4917)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4985..5089
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5090..5158,5159..5543,1..407)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5308..5327)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
       61 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
      121 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
      181 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
      241 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
      301 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
      361 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
      421 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
      481 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
      541 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
      601 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
      661 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      721 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      781 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
      841 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
      901 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
      961 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1021 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1081 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1141 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     1201 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     1261 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     1321 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     1381 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     1441 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     1501 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     1561 gctatgacat gattacgaat tcggcttcaa ggaccagctg tcagtcatgg tgataggctg
     1621 gctggggttg gcagggagct tgctctcctt ctcggagggc cggagcagcg tggggccaaa
     1681 gaccgtggcg aggttgtgca gggacatctt attgactgcc tccttctctg ccaccctttt
     1741 caggtggtcc agaaggaaaa ggaaggtgag caggttggcc tccggcaggg acagcagcag
     1801 gttgagcatg cagctctcct ttgcaaccgg gtctgaaaga gcgatgccct ctgcgaagtt
     1861 ggggtagaac tcgtcagtga agaggggctc gggcagctca cggaagtaca gcttcagcgt
     1921 gcctgcgatg gcgttcacgt ccatctcgct catcatcacc gacacatcct tgttattgac
     1981 gtcgaaggct gccttcagtg cctggatgtc cgtggccaca ccggacacgc ggtagatgcc
     2041 cacctcctcc atgcctcggc gctcgatctc ctccacgcac tggcgcacga tgtagggcac
     2101 cttggacctc tctctcttgg tgaccacagc aatcttgact ccgaagaccc ctgtctgttt
     2161 tcgggacggc atcctcttca agctgaactc cctgctgttg aacttgaccg agagctttac
     2221 ttcgatccca ttcatggcga tgacggtgcg ctgccagtct ctgtcctgca gggcctgcgg
     2281 gtccagctgg acctggccct tccccatgag tctgtccgtg ctctcgccgt cctccttggg
     2341 gatcttcgtc ttgttgtaac acttttcata gcacagtatc ctcagggtct gggagccctc
     2401 cagctctatc tcaaattcct cgttccagtt tggctcagct gtgtccctgt agacgcgcgt
     2461 ctttgcttta ttcacaaaat acccaaagga atccacctcc agggtgcagt acagatttga
     2521 actctgctta aatccagtgg ctgagtggac aagccgaatt cgagctcggt accgagctcg
     2581 gatccactag taacggccgc cagtgtgctg gaattcggct ttttccgtac cagccactac
     2641 ccctatgcag aggaagtgat gcagatgtgt gaccgctatg ggattgtggt catcgatgag
     2701 tgtcccggcg tgggcctggc gctgccgcag ttcttcaaca acgtttctct gcatcaccac
     2761 atgcaggtga tggaagaagt ggtgcgtagg gacaagaacc accccgcggt cgtgatgtgg
     2821 tctgtggcca acgagcctgc gtcccaccta gaatctgctg gctactactt gaagatggtg
     2881 atcgctcaca ccaaatcctt ggacccctcc cggcctgtga cctttgtgag caactctaac
     2941 tatgcagcag acaagggggc tccgtatgtg gatgtgatct gtttgaacag ctactactct
     3001 tggtatcacg actacgggca cctggagttg attcagctgc agctggccac ccagtttgag
     3061 aactggtata agaagtatca gaagcccatt attcagagcg agtatggagc agaaacgatt
     3121 gcagggtttc accaggatcc acctctgatg ttcactgaag agtaccagaa aagtctgcta
     3181 gagcagtacc atctgggtct ggatcaaaaa cgcagaaaat acgtggttgg agagctcatt
     3241 tggaattttg ccgatttcat gactgaacag tcaccgacga gagtgctggg gaataaaaag
     3301 gggatcttca ctcggcagag acaaccaaaa agtgcagcgt tccttttgcg agagagatac
     3361 tggaagattg ccaatgaaac caggtatccc cactcagtag ccaagtcaca atgtttggaa
     3421 aacagcccgt ttacaagccg aattctgcag atatccatca cactggcggc cgctcgagca
     3481 tgcatctaga gtcgactctt ccacctccat ggtgtcctcc ttcaaggtct tcacggccac
     3541 cgtcaggctg tatttcttcc acacgccctc gtacacctcc ccgtactggc ccccgcccag
     3601 cttgtgcttc atggtgatgt ccgtgcgttc catctcccac ttgtcgtagt tgggggacac
     3661 accatagaca gtgggcttgt tgcgctttgg ggctggataa tggagcgtgg tgatgagccc
     3721 gtcggccacc gttgaatgat gatgaaccaa ctcggccagg gtgttgaagc ggctctcgga
     3781 ggagacgtag agcttgccat cagaagcagt gttgatcctg taatggtaca ccctcccttc
     3841 gtatctcagc gagatggacc tctggccagg actgctctca ctctcacgca ccaagaagct
     3901 gccattgatc ccgctgctca gcagatactc agcggcattg cgggacacag gcccatggta
     3961 ccaggagtgt ttctccagac tgttgactgg cgtgatgtag ttgcttggga cccagccttg
     4021 gccatttttg gtttgggctt cacaccattc cccattgtga ttatagccta agacccggag
     4081 cttttcacct ttagttatgc ttagagtgtt atctccactg gccacaaaat catacagtgc
     4141 aacgaaaagg ttggggtcat tttcactggg tccagcgaga aggttttcct tggagttcca
     4201 acgagcggct tcactcagac cctgaggctc aaagtcagat gctactggcc gctgaagggc
     4261 ttcttccata aggagtaatc tcttcctcgt tgatgaagct ttcatcctgt cttctccctc
     4321 tcccttcttc accgtcatgg actgccgtcg gggtgtgatc tcctcattga tgctggacag
     4381 gaagttctgt gagatgcgga gggcgtcctg cagcaagggg tggtcagggt ggctggcagg
     4441 agtgtgcttc agcaagtcat ggaggacgtc gacctgcagg catgcaagct tggcactggc
     4501 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     4561 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     4621 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     4681 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     4741 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     4801 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     4861 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     4921 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     4981 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5041 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5101 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5161 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5221 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5281 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5341 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5401 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5461 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5521 ggagctaacc gcttttttgc aca
//
