LOCUS       sequence-41495-e         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Ahn S, Olive M, Aggarwal S, Krylov D, Ginty DD, Vinson C
  TITLE     A dominant-negative inhibitor of CREB reveals that it is a general 
            mediator of stimulus-dependent transcription of c-fos.
  JOURNAL   Mol Cell Biol. 1998 Feb . 18(2):967-77.
  PUBMED    9447994
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1998 Feb . 18(2):967-77."
COMMENT     Sequence Label: CMV500 A-C/EBP
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(282..314)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(318..341)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(361..380)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(362..380)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(425..628)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(454..474)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tatagaatag ggccctctag aagcttgaat tcgcctcacg cgcagttgcc catggccttg
       61 accaaggagc tctcaggcag ctggcggaag ataccccgca gcgtgtccag ttcacggctc
      121 agctgttcca cccgcttgcg caggcggtca ttgtcactgg tcaactccaa caccttctgc
      181 tcgagttcag cgttttcctg ctccagctct tcggcctctt tttccagctc ttcgttttca
      241 cgggccagtt cctcagcacg ttgttccagg tcaggatccc gacccatttg ctgtccacca
      301 gtcatgctag ccatatgctt gtcatcgtcg tccttgtagt ccatggtgga gcttgggtct
      361 ccctatagtg agtcgtatta atttcgataa gccagtaagc agtgggttct ctagttagcc
      421 agagagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
      481 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
      541 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
      601 cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
      661 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
      721 taccgtcatt gacgt
//
