LOCUS       sequence-41496-e         621 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Ahn S, Olive M, Aggarwal S, Krylov D, Ginty DD, Vinson C
  TITLE     A dominant-negative inhibitor of CREB reveals that it is a general 
            mediator of stimulus-dependent transcription of c-fos.
  JOURNAL   Mol Cell Biol. 1998 Feb . 18(2):967-77.
  PUBMED    9447994
REFERENCE   2  (bases 1 to 621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1998 Feb . 18(2):967-77."
COMMENT     Sequence Label: CMV500 A-C/EBP
FEATURES             Location/Qualifiers
     source          1..621
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             91..114
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             118..150
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(426..444)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(427..444)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(464..481)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    470..581
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctccaccatg gactacaagg acgacgatga caagcatatg
      121 gctagcatga ctggtggaca gcaaatgggt cgggatcctg acctggaaca acgtgctgag
      181 gaactggccc gtgaaaacga agagctggaa aaagaggccg aagagctgga gcaggaaaac
      241 gctgaactcg agcagaaggt gttggagttg accagtgaca atgaccgcct gcgcaagcgg
      301 gtggaacagc tgagccgtga actggacacg ctgcggggta tcttccgcca gctgcctgag
      361 agctccttgg tcaaggccat gggcaactgc gcgtgaggcg aattcaagct tctagagggc
      421 cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct
      481 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
      541 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
      601 cattctattc tggggggtgg g
//
