LOCUS       sequence-41504-e         673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Kobayashi T, Ooms LS, Ikizler M, Chappell JD, Dermody TS
  TITLE     An improved reverse genetics system for mammalian orthoreoviruses.
  JOURNAL   Virology. 2010 Mar 15;398(2):194-200. Epub 2009 Dec 29.
  PUBMED    20042210
REFERENCE   2  (bases 1 to 673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2010-03-15"; volume: "398(2)"; pages: "194-200. Epub 2009 Dec
            29"
COMMENT     Sequence Label: pT7-M1-S1-S2-S4T1L
FEATURES             Location/Qualifiers
     source          1..673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        571..589
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     571..588
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tcgtttacga agccgacgat caacgttgtc cggtatcact gaaggattac ttttccaata
       61 ctctagtagt tgacttttgg gtggcaacaa gcaatagacg gagtgatgta accaattctt
      121 gtggacgagt gcatcgataa cgtcgccagc ctcatatcca tctaacatat actgtaactg
      181 atccaacaca tagtcatgat cttgatatga aacgtcattc gcatcagccc cagcgtctac
      241 tccaaacgat tctagcagtc caatagcctc acttgaacgt gaatccacca ccgcaggaac
      301 tgcgatgtaa gccatgaccg cgaatagcta tagtgagtcg tattagctag cgatgaccct
      361 gctgattggt tcgctgacca tttccgggtg cgggacggcg ttaccagaaa ctcagaaggt
      421 tcgtccaacc aaaccgactc tgacggcagt ttacgagaga gatgataggg tctgcttcag
      481 taagccagat gctacacaat taggcttgta catattgtcg ttagaacgcg gctacaatta
      541 atacataacc ttatgtatca tacacatacg atttaggtga cactatagaa tacaagcttg
      601 catgcctgca ggtcgactct agaggatctc gatccggata tagttcctcc tttcagcaaa
      661 aaacccctca aga
//
