LOCUS       sequence-415369-        2909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTR4 plasmid with CDS of Pseudomonas syringae pv maculicola type 
            III effector AvrRpm1 without stop codon for C-terminal epitope tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Simranjit Kaur, Thomas Colby, Domenika Thieme, Carsten Proksch, 
            Susanne Matschi, eIvan Matic,, Lennart Wirthmueller
  TITLE     Untargeted proteomics identifies plant substrates of the 
            bacterial-derived ADP-ribosyltransferase AvrRpm1
  JOURNAL   bioRxiv 2023.09.25.558804
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.09.25.558804"
COMMENT     Sequence Label: pENTR4 AvrRpm1
FEATURES             Location/Qualifiers
     source          1..2909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     500..519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      764..850
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      942..969
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     977..1000
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    1019..1118
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1799..1898)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2010..2029)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2021..2830
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2088..2107)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
       61 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      121 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      241 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      301 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      361 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      421 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      481 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      541 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      601 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      661 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgctagc atggatctcg
      721 gggacgtcta actactaagc gagagtaggg aactgccagg catcaaataa aacgaaaggc
      781 tcagtcggaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
      841 taggacaaat ccgccgggag cggatttgaa cgttgtgaag caacggcccg gagggtggcg
      901 ggcaggacgc ccgccataaa ctgccaggca tcaaactaag cagaaggcca tcctgacgga
      961 tggccttttt gcgtttctac aaactcttcc tgttagttag ttacttaagc tcgggcccca
     1021 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
     1081 cttttttata atgccaactt tgtacaaaaa agcaggctcc acatgggctg tgtatcgagc
     1141 acttcaagaa gtactggata ttattccggt tacgaaaatc atgaggagcc aagggtggca
     1201 agtagcccga cgaacaggca tgatcgcggc tacgaaaccg attccgagca ttccaatgtt
     1261 aacttaatac ccaatgcgag gagagtgtat aaagagtctg ttttgtggca tggtacatct
     1321 atgcaatcaa aaatagattt gagaaggcaa gggtttgatg tgaatagaaa aactgatggt
     1381 gcaaccgagg gatctagaac atcgaactca gcccctacag acacgctcat cagaaatgct
     1441 agacgtcaca actacttaac agcgtatgat gtaacagcta agaattatgc tcgaagagca
     1501 gaccccgaat ccccagccct agttagaact ataggtgtca agagcaattt taatattgag
     1561 ctagatcctg aatcgaaaga cgagaatggt gaaatatccc aattcagctg cagaacgaca
     1621 gattcaatac ctaaaaaata cattgttgga agcaagagca gccagccggg agcagatgca
     1681 aaggttttta aaaaagaact ctccaatgcg ggctttgaag tttccacaag ccgtgcaggc
     1741 gagttattaa gagctgttca gtctgactca gaagatgact ttaactcgag atatctagac
     1801 ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
     1861 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctgc agctctggcc
     1921 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     1981 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     2041 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     2101 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     2161 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     2221 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     2281 tactcctggt gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     2341 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     2401 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     2461 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     2521 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     2581 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     2641 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     2701 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     2761 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     2821 gtttttctaa tcagaattgg ttaattggtt gtaacattat tcagattggg ccccgttcca
     2881 ctgagcgtca gaccccgtag aaaagatca
//
