LOCUS       sequence-415381-       10891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of SAMD9L Asn885fs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10891)
  AUTHORS   Russell AJ, Gray PE, Ziegler JB, Kim YJ, Smith S, Sewell WA, Goodnow
            CC
  TITLE     SAMD9L autoinflammatory or ataxia pancytopenia disease mutations 
            activate cell-autonomous translational repression.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Aug 24;118(34):e2110190118. doi: 
            10.1073/pnas.2110190118.
  PUBMED    34417303
REFERENCE   2  (bases 1 to 10891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-08-24"; volume: "118(34):e2110190118. 
            doi"; pages: " 10.1073/pnas.2110190118"
COMMENT     Sequence Label: pcDNA3.1+N-eGFP_SAMD9L_Asn885fs
FEATURES             Location/Qualifiers
     source          1..10891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             923..1636
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             join(923..925,926..1636)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(965..986)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1226..1245)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1573..1594
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6482..6499)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6488..6712
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6758..7186
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6845..6864)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7055..7076
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7195..7215)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7200..7529
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7380..7515
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7442..7461
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7596..8390
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7650..7669)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8260..8279
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8564..8685
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8601..8620)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8655..8674
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8734..8750)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8734..8750)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8747..8769)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8758..8774
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8782..8788,8789..8806,8807..8812))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8827..8848
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8965..8982)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9136..9724)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9216..9235)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9895..10686,10687..10755))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10518..10537
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10756..10860)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      961 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     1021 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     1081 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     1141 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     1201 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     1261 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     1321 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     1381 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     1441 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     1501 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     1561 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctcacggcat
     1621 ggacgagctg tacaagggta ccgagctcgg atccgaattc tgcagatatc cagcacagtg
     1681 gcggccgctt atgagtaaac aagtatctct acctgaaatg attaaagact ggaccaaaga
     1741 gcatgtgaaa aaatgggtaa atgaagacct taagattaat gagcaatacg ggcaaattct
     1801 gctcagtgaa gaagtaacag gattagtcct gcaggaatta actgagaagg accttgtaga
     1861 aatggggcta ccatggggtc cagcactttt gataaaacgt tcatacaaca aattgaatag
     1921 taagtcccct gaaagtgaca atcatgatcc gggacaatta gataattcaa aaccgtccaa
     1981 aacagaacac cagaaaaatc caaaacacac caaaaaggaa gaagaaaatt caatgtcatc
     2041 taatattgat tatgatccca gagagatcag agatatcaaa caagaagaat caattcttat
     2101 gaaagaaaat gtgttagatg aagtagcaaa tgctaaacac aagaaaaagg gtaagctaaa
     2161 acctgaacaa ttgacttgta tgccatatcc ttttgatcag ttccatgaca gccatcgcta
     2221 catagaacat tatactctac aacctgaaac aggagcactc aatctcattg atccaataca
     2281 tgagttcaaa gctctcacaa acacagaaac agccacggaa gtggacatta agatgaaatt
     2341 cagcaatgaa gtcttccgat ttgcatcagc ttgtatgaat tcacgcacca atggcaccat
     2401 ccattttgga gtcaaggaca aaccccatgg agaaattgtt ggtgtgaaaa tcaccagtaa
     2461 ggctgccttc attgaccact tcaatgtaat gatcaaaaag tattttgaag aaagtgagat
     2521 caatgaagcc aagaagtgta ttcgggagcc aaggtttgtg gaagtccttc tgcagaacaa
     2581 tacaccatct gacagatttg tcattgaagt tgatactatt ccaaaacact ctatatgtaa
     2641 tgataagtat ttctacattc agatgcaaat ttgtaaagat aaaatatgga aacaaaacca
     2701 aaatctttca ctgtttgtaa gagaaggggc tagctctagg gatatcctgg ccaattccaa
     2761 gcaacgggat gtagatttca aggcattttt acaaaattta aagtcactgg tagcatctag
     2821 aaaagaggct gaagaagagt atggaatgaa ggcaatgaag aaggagagtg aaggactaaa
     2881 gctggttaaa cttctcatag gaaaccgaga ctcactggat aattcatact atgactggta
     2941 cattcttgta acaaataaat gccatccaaa ccaaataaag cacttagatt ttttaaaaga
     3001 aattaaatgg tttgctgtgt tggagtttga tcctgaatct atgatcaatg gagtggtcaa
     3061 agcttacaaa gaaagtcggg tggcaaacct tcactttcca aatcaatatg aagacaagac
     3121 aactaacatg tgggagaaga tttctactct taatctttac caacagccca gctggatttt
     3181 ctgcaacggc agatcagacc tgaaaagcga gacatataaa cctctagaac cacatttatg
     3241 gcagagagaa agagcttcag aagtcaggaa actaatttta tttctcacag atgaaaatat
     3301 aatgacaaga ggaaaatttt tggtagtgtt tctattactc tcttcagtgg aaagcccagg
     3361 agatccactc attgaaactt tctgggcttt ctatcaagct ctcaaaggaa tggaaaatat
     3421 gttgtgtatc tctgtaaact cacatattta tcaacgatgg aaagatctac tacaaacaag
     3481 aatgaagatg gaagatgaac taacaaacca cagtatttcc actttaaata tagaactggt
     3541 aaacagcact atccttaaac taaaatcggt gactcggtca tcaagaaggt ttttgcccgc
     3601 ccgtggatct tcttcagtta tcctagagaa aaagaaagag gatgtcttga ctgcactgga
     3661 aatcctctgt gaaaatgagt gtacagagac agacatcgag aaagacaaat ctaaattcct
     3721 ggagtttaag aaatcaaaag aagaacactt ttatcgaggt ggcaaagtat cctggtggaa
     3781 cttctatttt tcttctgaaa actattcttc agattttgtt aaaagggaca gttatgaaaa
     3841 gcttaaagat ttaatacact gctgggcaga gtctcctaaa ccaatatttg caaaaatcat
     3901 caatctttat catcatccag gctgtggagg taccacactg gctatgcatg ttctctggga
     3961 cttaaagaaa aacttcagat gtgctgtgtt aaaaaacaag acaactgatt ttgcagaaat
     4021 tgcagagcaa gtgatcaatc tggtcaccta tagggcaaag agccatcagg attacattcc
     4081 tgtgcttctc cttgtggatg attttgaaga acaagaaaat gtctactttc tacaaaatgc
     4141 catccattcc gttttagcag aaaaggattt gcgatatgaa aaaacattgg taattatctt
     4201 aaactgcatg agatcccgga atccagatga aagtgcaaaa ttggcagaca gtattgcact
     4261 aaattaccaa ctttcttcca aggaacaaag agcttttggt gccaaactga aggaaattga
     4321 aaagcagcac aagaactgtg aaacttttat tccttcatga tcatgaaaag caattttgat
     4381 gaaacatata tagaaaatgt agtcaggaat atcctaaaag gacaggatgt tgacagcaag
     4441 gaagcacaac tcatttcctt cctggcttta ctcagctctt atgttactga ctctacaatt
     4501 tcagtttcac agtgtgaaat atttttggga atcatataca ctagtacacc ctgggaacct
     4561 gaaagcttag aagacaagat gggaacttat tctacacttc taataaaaac agaagttgca
     4621 gaatatggga gatacacagg tgtgcgtatc attcaccctc tgattgccct gtactgtcta
     4681 aaagaactgg aaagaagcta tcacttggat aaatgtcaaa ttgcattgaa tatattagaa
     4741 gagaatttat tctatgattc tggaatagga agagacaaat ttcaacatga tgttcaaact
     4801 cttctgctta caagacagcg caaggtgtat ggagatgaaa cagacactct gttttcccca
     4861 ttaatggaag ctttacagaa taaagacatt gaaaaggtct tgagtgcagg aagtagacga
     4921 ttcccacaaa atgcattcat ttgtcaagcc ttagcaagac atttctacat taaagagaag
     4981 gactttaaca cagctctgga ctgggcacgt caggccaaaa tgaaagcacc taaaaattcc
     5041 tatatttcag atacactagg tcaagtctac aaaagtgaaa tcaaatggtg gttggatggg
     5101 aacaaaaact gtaggagcat tactgttaat gacctaacac atctcctaga agctgcggaa
     5161 aaagcctcaa gagctttcaa agaatcccaa aggcaaactg atagtaaaaa ctatgaaacc
     5221 gagaactggt caccacagaa gtcccagaga cgatatgaca tgtataacac agcttgtttc
     5281 ttgggtgaaa tagaagttgg tctttacact atccagattc ttcagctcac tccctttttc
     5341 cacaaagaaa atgaattatc caaaaaacat atggtgcaat ttttatcagg aaagtggacc
     5401 attcctcctg atcccagaaa tgaatgttat ttggctctta gcaagttcac atcccaccta
     5461 aaaaatttac aatcagatct gaaaaggtgc tttgactttt ttattgatta tatggttctt
     5521 ctgaaaatga ggtataccca aaaagaaatt gcagaaatca tgttaagcaa gaaagtcagt
     5581 cgttgtttca ggaaatacac agaacttttc tgtcatttgg atccatgtct attacaaagt
     5641 aaagagagtc aattactcca ggaggagaat tgcaggaaaa agctagaagc tctgagagca
     5701 gataggtttg ctggactctt ggaatatctt aatccaaact acaaagatgc taccaccatg
     5761 gaaagtatag tgaatgaata tgccttccta ctgcagcaaa actcaaaaaa gcccatgaca
     5821 aatgagaaac aaaattccat tttggccaac attattctga gttgtctaaa gcccaactcc
     5881 aagttaattc aaccacttac cacgctaaaa aaacaactcc gagaggtctt gcaatttgta
     5941 ggactaagtc atcaatatcc aggtccttat ttcttggcct gcctcctgtt ctggccagaa
     6001 aatcaagagc tagatcaaga ttccaaacta atagaaaagt atgtttcatc cttaaataga
     6061 tccttcaggg gacagtacaa gcgcatgtgc aggtccaagc aggcaagcac acttttctat
     6121 ctgggcaaaa ggaagggtct aaacagtatt gttcacaagg ccaaaataga gcagtacttt
     6181 gataaagcac aaaatacaaa ttccctctgg cacagtgggg atgtgtggaa aaaaaatgaa
     6241 gtcaaagacc tcctgcgtcg tctaactggt caggctgaag gcaagctaat ctctgtagaa
     6301 tatggaacag aggaaaaaat aaaaatacca gtaatatctg tttattcagg tccactcaga
     6361 agtggtagga acatagaaag agtgtctttc tacctaggat tttccattga aggccctctg
     6421 gcatatgata tagaagtaat ttaactcgag tctagagggc ccgtttaaac ccgctgatca
     6481 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     6541 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     6601 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     6661 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     6721 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     6781 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6841 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6901 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6961 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7021 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7081 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7141 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     7201 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7261 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7321 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     7381 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7441 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     7501 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     7561 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     7621 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     7681 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     7741 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     7801 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     7861 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     7921 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     7981 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     8041 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     8101 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     8161 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     8221 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     8281 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     8341 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     8401 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     8461 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     8521 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     8581 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8641 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     8701 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     8761 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8821 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8881 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8941 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     9001 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     9061 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     9121 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9181 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9241 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9301 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     9361 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9421 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9481 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9541 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     9601 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9661 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9721 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9781 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9841 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9901 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9961 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    10021 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    10081 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    10141 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10201 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10261 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10321 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    10381 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    10441 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10501 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10561 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10621 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10681 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10741 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10801 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10861 cacatttccc cgaaaagtgc cacctgacgt c
//
