LOCUS       sequence-415396-       12600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel with an exofacial double
            HA tag in domain II and a D122A mutation that disrupts the 
            interaction with ?2?-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12600)
  AUTHORS   Dahimene S, Page KM, Kadurin I, Ferron L, Ho DY, Powell GT, Pratt 
            WS, Wilson SW, Dolphin AC
  TITLE     The alpha(2)delta-like Protein Cachd1 Increases N-type Calcium 
            Currents and Cell Surface Expression and Competes with 
            alpha(2)delta-1.
  JOURNAL   Cell Rep. 2018 Nov 6;25(6):1610-1621.e5. doi: 
            10.1016/j.celrep.2018.10.033.
  PUBMED    30404013
REFERENCE   2  (bases 1 to 12600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.10"; journalName: "Cell Rep"; date: 
            "2018-11-6- 6"; volume: "25"; issue: "6"; pages: "1610-1621.e5"
COMMENT     Sequence Label: Cav2.2 HA D122A pcDNA3
FEATURES             Location/Qualifiers
     source          1..12600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(527..545)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(528..545)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(565..582)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    571..795
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      841..1269
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(928..947)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1138..1159
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1278..1298)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1283..1612
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1463..1598
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1525..1544
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1679..2473
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1733..1752)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2343..2362
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2647..2768
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2684..2703)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2738..2757
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2817..2833)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2817..2833)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2830..2852)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2841..2857
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2865..2871,2872..2889,2890..2895))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2910..2931
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3048..3065)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3219..3807)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3299..3318)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3978..4769,4770..4838))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4601..4620
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4839..4943)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5018..5037)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5209..5588
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5589..5792
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5743..5763
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5837..5856
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5837..5855
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7657..7683
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7687..7713
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 acccccttga ccccccgccc cagcgtcacc tacaagacgg ccaactcctc gcctgtccac
       61 ttcgccgggg caccgagcgg cctccccgcc ttctcccccg gccggctcag ccggggcctc
      121 tcagaacaca acgccctgct gcagagggac ccgctcagcc ggcccctggc gcccggctcg
      181 cgcatcggct ccgaccccta cctggggcag cggctggaca gcgaggcgcc cgcccgtgcc
      241 ctgcccgagg acgcgcccgc ctttgaggag accgcggcca gcaactcggg ccgctcctcc
      301 cggacctcgt acgtgtcctc actgacctcg cagcccccgc cgctgcgccg cgtgcccaac
      361 ggctaccact gcactctggg gctgggcggg ggcggccggg cgcggcgcgg ctgccaccac
      421 cccgaccgcg accgccggtg ctagactagt tctagcaagc ttcgacgaat tctgcagata
      481 tccatcacac tggcggccgc tcgagcatgc atctagaggg ccctattcta tagtgtcacc
      541 taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
      601 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
      661 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
      721 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
      781 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
      841 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      901 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      961 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     1021 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     1081 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     1141 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     1201 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     1261 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     1321 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     1381 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     1441 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     1501 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     1561 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     1621 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
     1681 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     1741 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     1801 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     1861 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     1921 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     1981 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     2041 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     2101 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     2161 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     2221 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     2281 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     2341 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     2401 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     2461 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     2521 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     2581 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     2641 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     2701 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     2761 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     2821 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     2881 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     2941 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     3001 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     3061 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3121 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3181 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3241 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3301 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3361 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3421 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3481 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3541 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3601 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     3661 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3721 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3781 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3841 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3901 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3961 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4021 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4081 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4141 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4201 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4261 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     4321 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4381 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4441 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4501 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     4561 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     4621 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     4681 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     4741 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     4801 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     4861 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     4921 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     4981 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
     5041 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     5101 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     5161 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
     5221 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     5281 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     5341 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     5401 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     5461 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     5521 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     5581 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     5641 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     5701 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     5761 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt
     5821 actggcttat cgaaattaat acgactcact atagggagac ccaagcttgg taccgagctc
     5881 ggatccacta gtaacggccg ccagtgtgct ggaattcctc gagggtaccg ggccccccct
     5941 cgaggtcgaa gcttgatcat ggtccgcttc ggggacgagc tgggcggccg ctatgggggc
     6001 gcgggcggcg cggagcgggc ccggggcggc ggggccggcg gggctggcgg cccgggcccc
     6061 ggggggctgc cgccgggcca gcgggtcctc tacaaacagt cgatcgcgca gcgcgcgcgg
     6121 accatggcgc tgtacaaccc catcccggtc aagcagaact gcttcaccgt caaccgctcg
     6181 ctcttcgtct tcagcgagga caacgtcgtc cgcaaatacg ccaagcgcat caccgagtgg
     6241 cctccgttcg agtacatgat cctggccacc atcatcgcca actgcatcgt gctggccctg
     6301 gagcagcacc tccctgacgg ggccaagacg cctatgtccg agcgcctgga tgacaccgag
     6361 ccctacttca ttgggatctt ctgcttcgag gcggggatca agatcctggc cctgggcttt
     6421 gttctgcaca agggctccta tctgcggaac ggctggaacg tcatggactt cgtggtagtc
     6481 ctcacgggga ttcttgccac agccggaacc gacttcgacc tgcgaacgct gagggctgtg
     6541 cgtgtgctaa ggcccctgaa gctggtgtct gggattccga gcttgcaagt ggtgctcaag
     6601 tccatcatga aggccatggt cccgctgctg cagatcgggc tgctgctctt cttcgccatc
     6661 ctcatgtttg ccatcatcgg cctcgagttc tacatgggca agttccacaa ggcctgcttc
     6721 cccaacagca cagacccaga tcccgtgggt gacttcccct gtggcaagga ggccccggcc
     6781 cggctctgtg agggagacac ggagtgccgg gagtactggg ccggacccaa ctttggtatc
     6841 accaactttg acaacatcct gtttgccatc ttgaccgtgt tccagtgcat caccatggag
     6901 ggctggactg acatcctcta caatacaaac gatgcagccg gcaacacgtg gaactggctc
     6961 tacttcatcc ctctcatcat catcggctcc ttcttcatgc tcaacctggt gctgggtgtg
     7021 ctctcaggag agtttgctaa ggagcgcgag agagtggaga accgccgcgc cttcctgaag
     7081 ctccggcggc agcagcagat tgagcgggag ctcaacgggt acctggagtg gatcttcaag
     7141 gctgaggaag tcatgctggc tgaggaggac aggaacgcag aggagaagtc ccctctggat
     7201 gcagtgttga agagagcagc tgccaagaag agcagaagcg acctgatcca ggccgaggag
     7261 ggggagggcc ggctcacagg cctctgtgct cccggttccc cgttcgcccg tgccagcctg
     7321 aaaagcggga agacggagag ctcgtcgtac ttccggagga aggagaagat gttccgcttc
     7381 ttcatccggc gcatggtgaa ggcacagagc ttctactgga cggtgctgtg tgtggtggca
     7441 ctgaacacac tgtgcgtggc catggtgcac tacaaccagc cgcagcggct caccacggca
     7501 ttgtactttg cagagtttgt tttcctgggt ctcttcctca cagagatgtc cctgaagatg
     7561 tatggcctgg ggcccaggag ctacttccgg tcctccttca actgttttga ctttggggtc
     7621 atcgtgggca gtatctttga agtggtctgg gctgcttacc cctacgacgt ccccgactac
     7681 gccggctacc cgtacgacgt cccggactac gccggcgtcg acgtcaagcc agggacctcc
     7741 ttcgggatca gtgttctgcg ggctctgcgc ttactgagaa tcttcaaggt caccaaatac
     7801 tggaactccc tacggaacct ggtggtgtct ctgctcaact ccatgaagtc catcatcagc
     7861 ctcctcttcc tgctcttcct gttcattgtt gtctttgctc tgctggggat gcagctgttt
     7921 ggaggccagt tcaactttaa agacgagacc ccaacgacca actttgacac cttccctgct
     7981 gctatcctca ccgtcttcca gatcctgacc ggagaggact ggaatgcggt catgtaccac
     8041 gggatcgagt cgcaaggtgg cgtcagcaga ggaatgttct cctccttcta cttcattgtc
     8101 ctcactctgt ttgggaacta caccctgctg aatgtcttcc tggccattgc tgtggacaac
     8161 cttgccaatg ctcaggagct gaccaaggat gaagaggaga tggaagaagc agccaatcag
     8221 aaactcgcct tgcagaaggc caaggaagtg gctgaagtca gccccatgtc tgctgcgaac
     8281 atctccattg ccgccaggca gcagaactcg gccaaagcgc gctcggtgtg ggagcagcga
     8341 gccagccagc tgcggctgca gaacctgagg gccagctgcg aggcgctgta cagcgagatg
     8401 gaccccgagg agaggctgcg ctacgccacc gcgcgccacc tgcggcccga cgtgaagacg
     8461 cacctggacc ggccgctggt ggttgagccc ggccgcgacg ctccgcgggg ccccccgggg
     8521 gggaagtccc ggccggatgg ctcggaggcc cccgagggtg cggatccccc gcgcaggcat
     8581 caccggcacc gagacaagga caaggctccc gccacggtcc cctcggcggg ggaacaggac
     8641 agagccgaag ctctgagggc agagggcggg gagctggggc cccgcgagga gcgggggcgg
     8701 ccacgccgca gccgcagcaa ggaggccccg ggcgccccgg aggtgcgcag cgatcgcggc
     8761 cgtggcccgt gtcctgaagg tggtcggcgc caccaccggc gcgggtcacc ggaggaggca
     8821 gctgagaggg agccccgccg ccaccgcgcg caccggcacg ggcccgaccc gggcaaggag
     8881 ggcccagcgt ccgggaccag gggcgagcgg cgcgcacgac accgcacggg cccccgggcg
     8941 tgcccccggg aggctgagag cagcgaggag cctgcgcggc ggcaccgggc tcggcacaag
     9001 gcgccgccca cccaggagac cgcggagaag gacaaggagg ctgccgagaa ggggggcgag
     9061 gccacggagg ccgagaagga caaggaggct cggaaccacc agcccaagga gctgccctgt
     9121 gacctggagg ccatcggcat gctgggagtg ggcgctgtgc atacgctgcc cagcacctgt
     9181 ctgcagaagg tggaggagca gccagaggat gcggacaacc agcggaacgt cacccgcatg
     9241 ggtagtcagc ccccggacac cagcaccact gtgcacattc ctgtgacgct gaccggcccc
     9301 cccggggaga ccacggtagt gcccagtgga aacgtggacc tggaaagtca agcagagggg
     9361 aagaaggagg tggaaaccag cgatgtgatg aggagcggcc ctcggcccat tgtcccgtac
     9421 agttccatgt tctgtttaag cccaaccaac ctgctgcgcc gctgctgcca ctacattgtg
     9481 accatgcgat acttcgagat ggtcatcctc gtggtcatcg ccctgagcag catcgccctg
     9541 gctgccgagg accccgtgag aacagactct cccaggaata atgctctgaa gtacatggac
     9601 tacatcttca caggcgtctt caccttcgag atggtgataa agatgatcga tttgggactg
     9661 ctgctacacc ctggagccta tttccgggat ctgtggaaca ttctggactt cattgtggtc
     9721 agtggggccc tggtggcatt tgctttctca ggatccaaag ggaaagacat cagcaccatc
     9781 aaatccctga gagtcctgcg tgtgctgcgg ccactcaaga ccatcaagcg gctgcccaag
     9841 ctcaaggccg tgtttgactg tgtggtgaac tcactgaaaa acgtcctcaa catcctgatc
     9901 gtctacatgc tcttcatgtt catctttgcc gtcattgccg tgcagctctt caaagggaag
     9961 ttcttctact gcacggacga atccaaggag ctggagcgtg actgcagggg tcagtatttg
    10021 gattatgaga aggaggaagt ggaagctcaa ccgaggcagt ggaagaaata tgacttccac
    10081 tatgacaacg tgctctgggc tctgctaacg ctcttcacgg tgtccacagg ggaagggtgg
    10141 cccatggtgc tgaaacactc tgtggatgcc acctacgagg agcagggccc gagccccggg
    10201 taccgcatgg agctctccat cttctacgtg gtctactttg tggtctttcc cttcttcttt
    10261 gtcaacatct tcgtggcctt gatcatcatc accttccagg agcaggggga caaggtgatg
    10321 tccgagtgca gcctggagaa gaacgagagg gcttgcatcg actttgccat cagcgccaga
    10381 cccctgacgc ggtacatgcc ccagaacaag cagtcgttcc agtataagac gtggacgttc
    10441 gtggtgtccc caccttttga gtacttcatc atggctatga tcgccctcaa cactgtggtg
    10501 ctgatgatga agttctacga tgcgccgtac gagtatgagc taatgctgaa atgcctgaac
    10561 atcgtgttca cgtccatgtt ctccatggag tgtgtgctga agatcatcgc cttcggggtg
    10621 ctgaactatt tcagagatgc ctggaatgtc tttgactttg tcacggtgtt gggaagtatt
    10681 actgatattt tggtaacaga gattgcgaac aacttcatca acctcagctt cctgcgcctg
    10741 ttccgggccg cgcggctcat caagctgctg cgccagggct acaccatccg catcctgctt
    10801 tggaccttcg tgcagtcctt caaggcgctg ccctacgtgt gtctcctcat cgccatgctc
    10861 ttcttcatct acgccatcat tggcatgcag gtttttggga acatcgccct ggacgatgac
    10921 accagcatca acaggcacaa taacttccgg acgtttctgc aggccttgat gttgctgttc
    10981 aggagcgcca cgggggaggc ctggcacgag ataatgctgt cgtgcctcag cagccgggcc
    11041 tgcgacgagc actccaatgc cagcgagtgc ggcagcgact ttgcctactt ttacttcgtc
    11101 tccttcatct tcctctgctc ctttctgatg ttgaacctct ttgtggctgt gatcatggac
    11161 aattttgagt accttacacg ggactcttcc atcttagggc ctcaccacct ggatgagttc
    11221 atccgggtct gggctgaata cgacccggcc gcgtgtgggc gcatcagtta cagtgacatg
    11281 tttgagatgc tgaagcacat gtccccaccc ctggggctgg ggaagaagtg ccctgctcga
    11341 gtcgcgtaca agcgcctggt ccgcatgaac atgcccatct ccagtgagga catgacggtg
    11401 cacttcacat ccacgctgat ggctctcatt cggacagcgc tggacatcaa gctggcacca
    11461 gctgggacca agcagcacca gtgcgacgca gagctgagga aggagatttc ctgtgtgtgg
    11521 gccaatctgc cccagaagac cctggactta ctggtaccac cccataagcc tgacgagatg
    11581 acggtgggca aagtctatgc agctctgatg atcttcgact tctacaaaca gaacaaaacc
    11641 agcagagacc agactcagca agcccccgga ggcctgtccc agctgggccc cgtgtccctg
    11701 ttccacccgc tgaaggccac gctggagcag actcagccgg cgctgcgtgg agcccgggct
    11761 ttcctgcggc agaagagttc tgcctccctg agcaacggcg gggccgtaca aacccaggag
    11821 agcggcatca aggaatcggt ttcttggggc actcagagga cccaggatgt gctttgcgag
    11881 gccagggcac ccctggagcg gggccactca gctgagatcc cagtcgggca gcctgggaca
    11941 ctggctgtag atgtccagat gcagaacatg actctgagtg gccctgacgc cgagccgcag
    12001 ccagggctgg agagccaggg ccgagccgcc tccatgcccc gcctggccgc agaaacacag
    12061 cccgcgcctg acgccagccc catgaagcgc tccatctcca cgctggcccc gcgcccccac
    12121 acggcccggc tcggcagcac cgcgctggac cgcccagccc ccagccaggc cccgcaccat
    12181 caccaccacc gctgccaccg ccgcagggac aggaagcagc ggtccctgga gaagggtccc
    12241 agcctgtctg ctgacacaga tggggccccc gacagcacgg tggggccagg actgcccacg
    12301 ggggagggcc cccccggctg caggcgggag cgggagcgca ggcaggagcg gggccggtcc
    12361 caggagcgga ggcagccctc gtcctcctcc tcggagaagc atcgcttcta ctcctgtgac
    12421 cgcttcgggg gccgcgaacc cccacagccc aagccctccc tcagtagtca ccccacgtcg
    12481 ccgacggccg ggcaggagcc gggaccccac ccgcagggga gtggttcggt tcacgggagc
    12541 cccctgctgt ccacatctgg tgctagcacc ccgggccggg ggcggaggca gctcccccag
//
