LOCUS       sequence-415680-        9918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A variant CE1 construct with DCV HUH endonuclease, expressed from 
            CMV or T7 promoters. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9918)
  AUTHORS   Ferreira da Silva J, Tou CJ, King EM, Eller ML, Rufino-Ramos D, Ma 
            L, Cromwell CR, Metovic J, Benning FMC, Chao LH, Eichler FS, 
            Kleinstiver BP
  TITLE     Click editing enables programmable genome writing using DNA 
            polymerases and HUH endonucleases.
  JOURNAL   Nat Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x.
  PUBMED    39039307
REFERENCE   2  (bases 1 to 9918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x."
COMMENT     Sequence Label: HUHe variant click editor (CE1) -
            pCMV-T7-DCV-...
FEATURES             Location/Qualifiers
     source          1..9918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1062..1286
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1275..1292
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(1315..1332)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(1341..1361)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(3246..3266)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(3324..4907,4908..4910,4911..7424))
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(3324..7424)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     regulatory      7853..7862
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7869..7888)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7870..7888)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(7930..8133)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7959..7979)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8134..8513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8685..8704
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8783..8887
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8888..8956,8957..9748)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9106..9125)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccctag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtatc gagccccagc tggttctttc cgcctcagaa gccatagagc ccaccgcatc
     1081 cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc
     1141 ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga
     1201 aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac
     1261 agatggctgg caactagaag gcacagtcga ggctgatcag cgggtttaaa ctcaatggtg
     1321 atggtgatga tgaccggtca gactttcctc ttcttcttgg gagactcgaa ttcgctgccg
     1381 tcggcggttc tttttgagcc gccagagtgg gcctggtccc aattctctcc gcttccaacc
     1441 tcgaccagca gtggcacgtc cagtctggtg caattttcca tcagctggtg aatctgcttg
     1501 gccacagcat ccacgtcgtc cttgtgcacc tcaaacacca gctcgtcgtg cacctgcatg
     1561 atcatccgca ctctaggctg ctcggcctgc agccaggcgt ccacggcgat catggcccgc
     1621 ttgatgatgt cggcggcggt gccctgcata ggggcgttga tagcagctct ctcagcggcg
     1681 gctcttctgg cgccgttgct gctcttgatg tcaggcagat acagtctccg gccatccagg
     1741 gtttccacgt atccctgctc tttagcctgg gctctggtcc gttccatgta ctccagcacg
     1801 ccggggtacc gctcgaagta caggtccatg tatttctggg cctcctttct agggatgttg
     1861 agctgtctgg ccaggccaaa ggcgctcatg ccgtagatca ggccgaagtt aatggccttg
     1921 gcggaccttc tttgctcgct ggtcactgtt tccagaggca ggccgaacac ttcggcagcg
     1981 gtggctctgt ggatgtcctt gccttctgca aaagcggtca gcaggccctt gtcccgagac
     2041 agatgggcca tgattctcag ttctatctgg ctgtagtcag cagacacgat gacgtagtcc
     2101 tcaggggcaa tgaaagcctg tctgattctt ctgccctctt cgttccgcac tgggatgttt
     2161 tgcaggttag gatctgtgct gctcagccgg ccggtggctg tcacggcctg gtgataagat
     2221 gtatgcaccc ggccggtctt ggggttgatc atcagaggca gcttgtcggt gtatgtagac
     2281 ttcagcttgg ccagtcctct atattccagg atcactttgg gcagagggta atccagcgcc
     2341 agttcctcca gcacttcttc gctggtgctg ggggctccgc caggtgtctt tttcagaggc
     2401 ttaatgccct gcttctcgaa caggattgtt tgtagctgct tggtgctaga caggttgaac
     2461 tcctcgccgg cgatttcgtg ggccttcttt tccagctcgg ccagccgcag tgtcagttcc
     2521 tcgctgtggt tgtgcagcac cttggggtcg atcttcacgc cattcctctc gattctgctc
     2581 aggacgggca ccagaggcat ctcgatgttc tcgaacacgt ttagtgggcc cttgtgcttc
     2641 tgcagatcgg gccacatctt caggtgcagc tgaagggtca cgtcggcgtc ctcagcggcg
     2701 tatctgccgg cttcttccag tgcgatctga ttaaatgtca gctggttctt gccctttccg
     2761 gcgatctctt cgaaggtgat ggttttgtgc ttcagccacc gttcagccag gctgtccatg
     2821 tcgtggcggc cggccacgct gttcaggatg taagattcca gcatggtatc gaaggcgatg
     2881 cctctcagct cgatgccgta attagccagg attcccctat cgtatttcag gttctgtccc
     2941 accttcaggg ccttctcgtc ctccagcagg ggcttcagga gctccagtgc tctctctctg
     3001 ctgatttgat ctggggcatc caggtaatcg tgggccacgg ggatgtaggc ggccacgcca
     3061 ggctcgatgg cgaaagacag gcccaccagg ttggcgctga tgttatctaa ggagtcggtg
     3121 gcggtggcga aggcgaacac aggggctttt tccagtttag cgatccaagc cttcagtgtc
     3181 tcttcgtcca ggatggtcac gtagttgtcg taggagataa cgcttccgcc gctagagcct
     3241 ccgctcacct ttcttttctt cttagggctc tcgaattcag agccgtcggc ggtccgcttg
     3301 ctgccaccag agcttccgcc ggagtcacct cccagctgag acaggtcgat ccgtgtctcg
     3361 tacaggccgg tgatgctctg gtggatcagg gtggcgtcca gcacctcttt ggtgctggtg
     3421 tacctcttcc ggtcgatggt ggtgtcaaag tacttgaagg cggcaggggc tcccagattg
     3481 gtcagggtaa acaggtggat gatattctcg gcctgctctc tgatgggctt atcccggtgc
     3541 ttgttgtagg cggacagcac tttgtccaga ttagcgtcgg ccaggatcac tctcttggag
     3601 aactcgctga tctgctcgat gatctcgtcc aggtagtgct tgtgctgttc cacaaacagc
     3661 tgtttctgct cattatcctc gggggagccc ttcagcttct catagtggct ggccaggtac
     3721 aggaagttca catatttgga gggcagggcc agttcgtttc ccttctgcag ttcgccggca
     3781 gaggccagca ttctcttccg gccgttttcc agctcgaaca gggagtactt aggcagcttg
     3841 atgatcaggt cctttttcac ttctttgtag cccttggctt ccagaaagtc gatgggattc
     3901 ttctcgaagc tgcttctttc catgatggtg atccccagca gctctttcac actcttcagt
     3961 ttcttggact tgcccttttc cactttggcc accaccagca cagaataggc cacggtgggg
     4021 ctgtcgaagc cgccgtactt cttagggtcc cagtccttct ttctggcgat cagcttatcg
     4081 ctgttcctct tgggcaggat agactctttg ctgaagccgc ctgtctgcac ctcggtcttt
     4141 ttcacgatat tcacttgggg catgctcagc actttccgca cggtggcaaa atcccggccc
     4201 ttatcccaca cgatctcccc ggtttcgccg tttgtctcga tcagaggccg cttccggatc
     4261 tcgccgttgg ccagggtaat ctcggtcttg aaaaagttca tgatgttgct gtagaagaag
     4321 tacttggcgg tagccttgcc gatttcctgc tcgctcttgg cgatcatctt ccgcacgtcg
     4381 tacaccttgt agtcgccgta cacgaactcg ctttccagct tagggtactt tttgatcagg
     4441 gcggttccca cgacggcgtt caggtaggcg tcgtgggcgt ggtggtagtt gttgatctcg
     4501 cgcactttgt aaaactggaa atccttccgg aaatcggaca ccagcttgga cttcagggtg
     4561 atcactttca cttcccggat cagcttgtca ttctcgtcgt acttagtgtt catccgggag
     4621 tccaggatct gtgccacgtg ctttgtgatc tgccgggttt ccaccagctg tctcttgatg
     4681 aagccggcct tatccagttc gctcaggccg cctctctcgg ccttggtcag attgtcgaac
     4741 tttctctggg taatcagctt ggcgttcagc agctgccgcc agtagttctt catcttcttc
     4801 acgacctctt cggagggcac gttgtcgctc ttgccccggt tcttgtcgct tctggtcagc
     4861 accttgttgt cgatggagtc gtccttcaga aagctctgag gcacgatagc gtccacatcg
     4921 tagtcggaca gccggttgat gtccagttcc tggtccacgt acatatcccg cccattctgc
     4981 aggtagtaca ggtacagctt ctcgttctgc agctgggtgt tttccacggg gtgttctttc
     5041 aggatctggc tgcccagctc tttgatgccc tcttcgatcc gcttcattct ctcgcggctg
     5101 ttcttctgtc ccttctgggt ggtctggttc tctctggcca tttcgatcac gatgttctcg
     5161 ggcttgtgcc ggcccatcac tttcacgagc tcgtccacca ccttcactgt ctgcaggatg
     5221 cccttcttaa tggcggggct gccggccaga ttggcaatgt gctcgtgcag gctatcgccc
     5281 tggccggaca cctgggcttt ctggatgtcc tctttaaagg tcaggctgtc gtcgtggatc
     5341 agctgcatga agtttctgtt ggcgaagccg tcggacttca ggaaatccag gattgtcttg
     5401 ccggactgct tgtcccggat gccgttgatc agcttccggc tcagcctgcc ccagccggtg
     5461 tatctccgcc gcttcagctg cttcatcact ttgtcgtcga acaggtgggc ataggttttc
     5521 agccgttcct cgatcatctc tctgtcctca aacagtgtca gggtcagcac gatatcttcc
     5581 agaatgtcct cgttttcctc attgtccagg aagtccttgt ccttgataat tttcagcaga
     5641 tcgtggtatg tgcccaggga ggcgttgaac cgatcttcca cgccggagat ttccacggag
     5701 tcgaagcact cgattttctt gaagtagtcc tctttcagct gcttcacggt cactttccgg
     5761 ttggtcttga acagcaggtc cacgatggcc tttttctgct cgccgctcag gaaggcgggc
     5821 tttctcattc cctcggtcac gtatttcact ttggtcagct cgttatacac ggtgaagtac
     5881 tcgtacagca ggctgtgctt gggcagcacc ttctcgttgg gcaggttctt atcgaagttg
     5941 gtcatccgct cgatgaagct ctgggcggaa gcgcccttgt ccaccacttc ctcgaagttc
     6001 cagggggtga tggtttcctc gctctttctg gtcatccagg cgaatctgct gtttcccctg
     6061 gccagagggc ccacgtagta ggggatgcgg aaggtcagga tcttctcgat cttttcccgg
     6121 ttgtccttca ggaatgggta aaaatcttcc tgccgccgca gaatggcgtg cagctctccc
     6181 aggtggatct ggtgggggat gctgccgttg tcgaaggtcc gctgcttccg cagcaggtcc
     6241 tctctgttca gcttcacgag cagttcctcg gtgccgtcca tcttttccag gatgggcttg
     6301 atgaacttgt agaactcttc ctggctggct ccgccgtcaa tgtagccggc gtagccgttc
     6361 ttgctctggt cgaagaaaat ctctttgtac ttctcaggca gctgctgccg cacgagagct
     6421 ttcagcaggg tcaggtcctg gtggtgctcg tcgtatctct tgatcataga ggcgctcagg
     6481 ggggccttgg tgatctcggt gttcactctc aggatgtcgc tcagcaggat ggcgtcggac
     6541 aggttcttgg cggccagaaa caggtcggcg tactggtcgc cgatctgggc cagcaggttg
     6601 tccaggtcgt cgtcgtaggt gtccttgctc agctgcagtt tggcatcctc ggccaggtcg
     6661 aagttgctct tgaagttggg ggtcaggccc aggctcaggg caatcaggtt tccgaacagg
     6721 ccattcttct tctcgccggg cagctgggcg atcagatttt ccagccgtct gctcttgctc
     6781 agtctggcag acaggatggc cttggcgtcc acgccgctgg cgttgatggg gttttcctcg
     6841 aacagctggt tgtaggtctg caccagctgg atgaacagct tgtccacgtc gctgttgtcg
     6901 gggttcaggt cgccctcgat caggaagtgg ccccggaact tgatcatgtg ggccagggcc
     6961 agatagatca gccgcaggtc ggccttgtcg gtgctgtcca ccagtttctt tctcaggtgg
     7021 tagatggtgg ggtacttctc gtggtaggcc acctcgtcca cgatgttgcc gaagatgggg
     7081 tgccgctcgt gcttcttatc ctcttccacc aggaaggact cttccagtct gtggaagaag
     7141 ctgtcgtcca ccttggccat ctcgttgctg aagatctctt gcagatagca gatccggttc
     7201 ttccgtctgg tgtatcttct tctggcggtt ctcttcagcc gggtggcctc ggctgtttcg
     7261 ccgctgtcga acagcagggc tccgatcagg ttcttcttga tgctgtgccg gtcggtgttg
     7321 cccagcacct tgaatttctt gctgggcacc ttgtactcgt cggtgatcac ggcccagccc
     7381 acagagttgg tgccgatgtc caggccgatg ctgtacttct tgtcgctgcc gccgctgctc
     7441 tcaggggtgg cggactcaga tgtgccgggt gtctcggagc cgctagagcc tccgctcaca
     7501 gcgctggtgg cctcggccag gtcgctgctt ctgcccttag acacaggctc gcccacccgg
     7561 agataggtgc tttccttggc acagtattcc tcgttgtcct catcggagcc tctggctctg
     7621 ctcagccaag cccgtccgcc caggctctct tccagagcgg cggctctggc gttgcttctc
     7681 aggttcagga agccctgcag gtgaggtgtg ccattggctc ccttttcttc gcccacgatg
     7741 gcgaacttgc aattatccag ggtgcagaat tccagcacgt gcacgtagtc ctcgaaggtg
     7801 gggttgttga tggtaaagac ccaccgcttg tagctgtagt tgccgctttt ggccatggtg
     7861 gcggctctcc ctatagtgag tcgtattagc ggccgcggat ctctagcgga tctgacggtt
     7921 cactaaacca gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
     7981 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
     8041 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
     8101 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
     8161 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     8221 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     8281 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     8341 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     8401 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg
     8461 aactaatgac cccgtaattg attactatta ataactagtc aataatcaat gtcaacgcgt
     8521 atatctggcc cgtacatcgc gaagcagcgc aaaacgccta accctaagca gattcttcat
     8581 gcaattgtcg gtcaagcctt gccttgttgt agcttaaatt ttgctcgcgc actactcagc
     8641 gacctccaac acacaagcag ggagcagata ctggcttaac tatgcggcat cagagcagat
     8701 tgtactgaga gtcgacccta ggggatcggg agatcgatct cccgatccgt cgacgtcagg
     8761 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8821 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     8881 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     8941 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     9001 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     9061 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     9121 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     9181 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     9241 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     9301 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     9361 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     9421 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     9481 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     9541 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     9601 tggttcgcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     9661 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     9721 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     9781 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     9841 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     9901 aaagatcaaa ggatcttc
//
