LOCUS       sequence-41577-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Kwon Y, Hofmann T, Montell C
  TITLE     Integration of phosphoinositide- and calmodulin-mediated regulation 
            of TRPC6.
  JOURNAL   Mol Cell. 2007 Feb 23. 25(4):491-503.
  PUBMED    17317623
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2007 Feb 23. 25(4):491-503."
COMMENT     Sequence Label: pcDNA3-AKT-PH-GFP
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(513..532)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(514..532)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(577..780)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(606..626)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gctctcgccc ttgctcacca tggtggcgat ggatccgttc atggtcacgc ggtgcttggg
       61 cttggccagg gacacctcca tctcttcagc ccctgagttg tcactgggtg agcccgaccg
      121 gaagtccatc tcctcctcct cctgcttctt gaggccgtca gccacagtct ggatggcggt
      181 tgtccactcc tcccgctcct caggagtctc cacatggaag gtgcgttcga tgacagtggt
      241 ccactgcagg cagcggatga tgaaggtgtt gggccggggc cgctccgtct tcatcagctg
      301 gcactgcgcc acagagaagt tgttgagggg agcctcacgt tggtccacat cctgcggccg
      361 ctccttgtag ccaatgaagg tgccatcatt cttgaggagg aagtagcgtg gccgccaggt
      421 cttgatgtac tcccctcgtt tgtgcagcca accctccttc acaatagcca cgtcgctcat
      481 ggtggcgaat tcgcggccgc aagcttgggt ctccctatag tgagtcgtat taatttcgat
      541 aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat agacctccca
      601 ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa
      661 gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg
      721 aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac
      781 catggtaata gcgatgacta atacgtagat g
//
