LOCUS       sequence-415917-       11433 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter gene expression in B. dendrobatidis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11433)
  AUTHORS   Kalinka E, Brody SM, Swafford AJM, Medina EM, Fritz-Laylin LK
  TITLE     Genetic transformation of the frog-killing chytrid fungus 
            Batrachochytrium dendrobatidis.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Jan 23;121(4):e2317928121. doi: 
            10.1073/pnas.2317928121. Epub 2024 Jan 18.
  PUBMED    38236738
REFERENCE   2  (bases 1 to 11433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-01-23"; volume: "121(4):e2317928121. 
            doi"; pages: " 10.1073/pnas.2317928121. Epub 2024 Jan 18"
COMMENT     Sequence Label: pGI3EM17
FEATURES             Location/Qualifiers
     source          1..11433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     206..228
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     220..237
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     221..237
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             536..1561
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             536..1561
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             1577..2092
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     terminator      2114..2301
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2169..2193)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2316..2332)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2316..2332)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2329..2351)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2340..2356
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2364..2370,2371..2388,2389..2394))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2409..2430
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    2607..2631
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(2913..2932)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2924..3739
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2924..3739
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2997..3016)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3834..4422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4323..4342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4576..4593
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4608..4748
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      5092..5286
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5352..6425)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6854..7483)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     rep_origin      8644..9986
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9607..9654)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10027..10045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10248..10463
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             10464..11267
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      121 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      181 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      241 aagcttttat gctccaagcg gagacgatga ggccgatatt acagtggctt agtagcgtgt
      301 acaatatttc cataaaaggc tacatggtga gtttttcacg tgaccgtaat tagtcccaat
      361 taggctttat tgtatattgc tactaagcat cgttaaatga cctgctcgaa gcttcgtcta
      421 taaaaggcgg gcgtgtgtgc gcttcccccg ctacgtcttt ttcacaaaca ctttgcgact
      481 tgaccaagac ctttcacccg caccactctc atccatcaca cacacaaatt aattaatggg
      541 taaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
      601 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
      661 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
      721 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
      781 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
      841 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
      901 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
      961 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     1021 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     1081 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     1141 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     1201 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     1261 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     1321 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     1381 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     1441 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     1501 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     1561 aggaggagga ggatccatgg tcttcacact cgaagatttc gttggggact ggcgacagac
     1621 agccggctac aacctggacc aagtccttga acagggaggt gtgtccagtt tgtttcagaa
     1681 tctcggggtg tccgtaactc cgatccaaag gattgtcctg agcggtgaaa atgggctgaa
     1741 gatcgacatc catgtcatca tcccgtatga aggtctgagc ggcgaccaaa tgggccagat
     1801 cgaaaaaatt tttaaggtgg tgtaccctgt ggatgatcat cactttaagg tgatcctgca
     1861 ctatggcaca ctggtaatcg acggggttac gccgaacatg atcgactatt tcggacggcc
     1921 gtatgaaggc atcgccgtgt tcgacggcaa aaagatcact gtaacaggga ccctgtggaa
     1981 cggcaacaaa attatcgacg agcgcctgat caaccccgac ggctccctgc tgttccgagt
     2041 aaccatcaac ggagtgaccg gctggcggct gtgcgaacgc attctggcgt aaaggcgcgc
     2101 cacttctaaa taagcgaatt tcttatgatt tatgattttt attattaaat aagttataaa
     2161 aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt
     2221 cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagtatg aggtcgctct
     2281 tattgaccac acctctaccg ggaattcgta atcatgtcat agctgtttcc tgtgtgaaat
     2341 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     2401 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     2461 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     2521 ttgcgtattg gctagagcaa ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg
     2581 ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc
     2641 caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca
     2701 gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg
     2761 atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga
     2821 gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcca gatcatgaac
     2881 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     2941 ggaaacgtct tgctctaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     3001 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa
     3061 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     3121 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     3181 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc
     3241 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     3301 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     3361 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     3421 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     3481 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     3541 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     3601 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     3661 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     3721 gctcgatgag tttttctaag aattaattca tgatcctgca tgaccaaaat cccttaacgt
     3781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3961 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     4021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4141 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     4261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4441 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     4501 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     4561 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     4621 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     4681 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     4741 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     4801 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     4861 caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt
     4921 ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc agccattttt
     4981 gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac
     5041 cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag ttatgcacag
     5101 gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct
     5161 tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta cggctttggg
     5221 ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat gtacgtgcta
     5281 tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg ccctagcatc
     5341 tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg
     5401 actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg caggtcattt
     5461 gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc gctgccactg
     5521 cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc
     5581 aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc
     5641 agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc
     5701 tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct aatcaaggct
     5761 tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg ctgcatggca
     5821 acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg ctttccgcca
     5881 tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc
     5941 ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga aaccgccatc
     6001 agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga aacctctacg
     6061 tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg cttcgacaga
     6121 cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc atagagcatc
     6181 ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg
     6241 gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg ccacgattca
     6301 ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc
     6361 tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg
     6421 ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg cagacaaccc
     6481 agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc acggcgtcgg
     6541 tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt catctactca
     6601 tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag cagctcggta
     6661 atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac
     6721 tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt tgcagccttg
     6781 ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct cattttctct
     6841 ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc tggacctcgc
     6901 gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg gcagtgcctg
     6961 ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac ccggccagcg
     7021 cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg
     7081 cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc aggtcggcgg
     7141 tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg gctgccttga
     7201 tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc
     7261 ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca acggttagcg
     7321 gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg gaatcgacta
     7381 acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg atggtcgtct
     7441 tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc ccttgcgtat
     7501 ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc tgttttactc
     7561 aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca agctggccgg
     7621 ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc gcacggttga
     7681 gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct cgatctcttc
     7741 ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg
     7801 cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc acggaaggca
     7861 ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg
     7921 gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg
     7981 atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc
     8041 acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc
     8101 tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg cgtggtggtg
     8161 tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat gcgctcggca
     8221 atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg
     8281 tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg cctggtgccg
     8341 gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc ccgttggttg
     8401 gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc
     8461 ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa
     8521 cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac
     8581 ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagga aaagtgccac
     8641 ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt
     8701 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta
     8761 ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc
     8821 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag
     8881 cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag
     8941 agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata
     9001 atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt
     9061 tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta
     9121 ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc
     9181 gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt
     9241 cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt
     9301 acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct
     9361 aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca
     9421 aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata tatagcaaag
     9481 agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca
     9541 gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag
     9601 cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa cttcaaagcg
     9661 tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt
     9721 cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa gaacggcata
     9781 gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga tgaaaggtag
     9841 tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct tccttcagca
     9901 ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc atccttcaat
     9961 gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat gacattaacc
    10021 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
    10081 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
    10141 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
    10201 tatgcggcat cagagcagat tgtactgaga gtgcaccata ccaccttttc aattcatcat
    10261 ttttttttta ttcttttttt tgatttcggt ttccttgaaa tttttttgat tcggtaatct
    10321 ccgaacagaa ggaagaacga aggaaggagc acagacttag attggtatat atacgcatat
    10381 gtagtgttga agaaacatga aattgcccag tattcttaac ccaactgcac agaacaaaaa
    10441 cctgcaggaa acgaagataa atcatgtcga aagctacata taaggaacgt gctgctactc
    10501 atcctagtcc tgttgctgcc aagctattta atatcatgca cgaaaagcaa acaaacttgt
    10561 gtgcttcatt ggatgttcgt accaccaagg aattactgga gttagttgaa gcattaggtc
    10621 ccaaaatttg tttactaaaa acacatgtgg atatcttgac tgatttttcc atggagggca
    10681 cagttaagcc gctaaaggca ttatccgcca agtacaattt tttactcttc gaagacagaa
    10741 aatttgctga cattggtaat acagtcaaat tgcagtactc tgcgggtgta tacagaatag
    10801 cagaatgggc agacattacg aatgcacacg gtgtggtggg cccaggtatt gttagcggtt
    10861 tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg ccttttgatg ctagcagaat
    10921 tgtcatgcaa gggctcccta tctactggag aatatactaa gggtactgtt gacattgcga
    10981 agagcgacaa agattttgtt atcggcttta ttgctcaaag agacatgggt ggaagagatg
    11041 aaggttacga ttggttgatt atgacacccg gtgtgggttt agatgacaag ggagacgcat
    11101 tgggtcaaca gtatagaacc gtggatgatg tggtctctac aggatctgac attattattg
    11161 ttggaagagg actatttgca aagggaaggg atgctaaggt agagggtgaa cgttacagaa
    11221 aagcaggctg ggaagcatat ttgagaagat gcggccagca aaactaaaaa actgtattat
    11281 aagtaaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta tatcagttat
    11341 tacctggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
    11401 ccgattattc taataaacgc tcttttctct tag
//
