LOCUS       sequence-41626-e        6755 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Johnson BE, Glauser DA, Dan-Glauser ES, Halling DB, Aldrich RW, 
            Goodman MB
  TITLE     Alternatively spliced domains interact to regulate BK potassium 
            channel gating.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Nov 2.
  PUBMED    22049343
REFERENCE   2  (bases 1 to 6755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2011 Nov 2."
COMMENT     Sequence Label: mg178
FEATURES             Location/Qualifiers
     source          1..6755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3579..3595)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3579..3595)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     3'UTR           3618..3743
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     primer_bind     complement(3823..3842)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3824..3842)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3852..3869)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3852..3868)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3861..3883)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(4009..4464)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4146..4167)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4358..4377
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4491..4595
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4596..4664,4665..5456)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4814..4833)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5627..6215
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6116..6135
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6369..6386
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6503..6524
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6539..6544,6545..6562,6563..6569)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6577..6593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6582..6604
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6601..6617
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6601..6617
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6636..6656
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6638..6656
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     5'UTR           6697..6739
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
ORIGIN
        1 atgggcgaga tttactcgcc ttcgcagtcg aagggcttca atcaaccata tggatacccg
       61 atgaattgta accttagtcg agtattcatg gagatgactg aagaagatcg aaagtgcctg
      121 gaggagcgca aatattggtg ttttttactg agcagcatca ccacattctg cgcctcaatg
      181 atattggtcg taatatggcg agtggtgaca cacctgtgct gtcaacgccg agaaaaagag
      241 tttgtggagc ccatacctgc tccggaagcc gtccaaataa atatgaacgg gtcgaagcac
      301 gcgccaagtg aaaccgaccc gtttttgaag caacaagagg aaaaacactt gggatggatg
      361 acagaagcca aggactgggc tggcgagctg atatccggac aaagtctgac gggacggttt
      421 ctggtacttt tggtgttcat tttatcgatt ggatcgttga ttatttattt ttacgacgct
      481 agtttccaaa acttccaagt agaaacgtgc atcccgtggc aggacagtcc ctcgcaacaa
      541 atcgacctgg gcttcaacat attttttctc gtctattttt tcattagatt catcgccgcg
      601 tccgacaaag tttggtttct gcttgaaatg tacagctgga ttgatttttt cacgattccg
      661 ccaagcttcg tggcaattta cttgcagagg aattggctcg gattccgttt cctccgtgct
      721 cttcgcctca tgaccgtacc cgacattcta cagtacctca acatcctgaa aacatcttca
      781 tcaatccgat tgacacagtt ggtcacaatt ttcgtggcgg tttgtctgac tggagccggc
      841 ttggtgcact tactggaaaa ttctggggac ttcttcaaag gatttataaa tccgcacaga
      901 atcacttatg cggactctgt gtactttgta ctggtcacaa tgtctactgt cggatatgga
      961 gatatctatt gtacgacgct ttgcggccga cttttcatga ttttcttcat tctgttcggt
     1021 ttggccatgt ttgcaagtta cgtaccagaa attgccgatt tgattggaaa ccggcaaaaa
     1081 tacggtgggg agtacaaagg agagcacggg aagaagcaca tagtggtctg tggccatatc
     1141 acctacgatt cggtgtccca ttttcttcaa gatttcctac acgaggaccg tgatgacgtg
     1201 gatgtcgaag tggtgttttt gcatcgtgtc gtgccggatt tggagctgga aggcttgttt
     1261 aagcggcatt tcacaaaagt cgaatttttc acggggactg tcatggattc tctggatctt
     1321 agcagagtca agatcgggga cgccgacgcc tgcctggtcc ttgccaacaa gtacagtacc
     1381 aatccggacg ccgaggacgc cgcaaacatt atgcgtgtaa tttcgatcaa aaactactca
     1441 tcggacatca gagttattgt gcaactaatg caatatcaca ataaggccta ccttctaaat
     1501 atcccctcct gggactggaa acgaggtgat gacgtcatct gtctcgcgga gcttaagctc
     1561 ggattcattg ctcaaagctg tctggcaccc ggattctcta cgatgatggc taatttattt
     1621 gcaatgagaa gtttcaaaac gtctcctcac acccctctct ggcttaacga ctacttacgt
     1681 ggtgccggaa tggaaatgta cacggaaagt ctgagtcctt cgtttgcgaa tatgtcattt
     1741 ccagaagctg caaacctatt attcaaccgc ctggggcttc ttcttctggc gattgagctt
     1801 aaagatgagg aaaataaaga atgcaatatt gcaatcaatc caggcccaca tattgtcatc
     1861 cagcctcaaa ctcaaggatt tttcattgct cagagcgctg acgaggtgaa gcgggcgttt
     1921 ttctggtgta agcagtgtca tgacgatatt aaggacgttt ccttgatcaa aaaatgtaaa
     1981 tgcaaaaatt tggccctctt ccgtcgcaac accaaacact cgacagcggc ccgtgattat
     2041 tcggactttg acgctctgtt ttatcagaat gacgcaagag ccaccgatgt gctccagcag
     2101 ttccagccac aagcgccagc aggtccgatg ggccatttgg gccaacaagt tcagctaagg
     2161 atgattaatc aacagagttc gacgagtgat actcatctca acacgaaatc ccttcgattt
     2221 gcctatgaaa tcaaaaaact catgccatct tccggaggac gccgaaattc gatgagtatt
     2281 ccgccagacg gcaggggtgt cgattttagc aaggattttg agcagcaatt ccaagacatg
     2341 aaatatgaca gtactggaat gttccactgg tgtccatccc gtaacctaga ggactgtgtt
     2401 ctggagcgcc accaggccgc tatgaccgta ctcaacggcc acgtggtggt ttgtttgttc
     2461 gctgatcagg attctccgct aattgggctc cggaacttca taatgccact aagaagctca
     2521 aacttccatt atcatgagct caaacacgtg gttattgtgg gagacctgga atatttgagg
     2581 aaagagtgga agacgctgta caacttgccg aaaatctcga ttttgaatgg ctccccgcta
     2641 tctcgagcag atttacgcgc cgtcaacatt aacctctgcg acatgtgtgt cataatctct
     2701 gcccgggtcc caaataccga agataccact ttggcagata aggaagcgat tctggcatca
     2761 ttgaacatta aagctatgca atttgatgat acccttggct ttttcccgat gaggcatcaa
     2821 actggcgatc ggagcccgct gggctccccg atttccatgc agaagaaggg cgccaagttt
     2881 gggaccaatg tgccgatgat tacggagctt gttaacgact caaatgtgca attcctcgac
     2941 caggacgacg acgacgatcc ggacaccgag ctctacctca cgcagccctt cgcctgcgga
     3001 actgccttcg ccatttcagt gttggattct ttgatgagta ctacctactt caacgactcg
     3061 gccctgaccc tcatccgaac tctggtcact ggtggagcca caccggagct tgaacttatt
     3121 ctggcagaag gcgccgggct ccgaggtggc tacagtaccc cggagaccct ctcaaacaga
     3181 gatcggtgcc gaattgctca gatttcgctg caggataacc cgtacgatgg agttgtgcac
     3241 aacactacct atggtgcaat gttcacgata gctctgagga ggtacggcca actttgtatc
     3301 gggctgtata ggcttcacga ccaggataat ccggactcta tgaagcgata tgttatcact
     3361 aatcctccag cagaattgag gattaaaaat actgattacg tctacgtact tgaacaattc
     3421 gatccgggac tggagtacga gccgggcaaa cgacactttt agggcctaat tttatccgat
     3481 tttcttgcta caaactacgg gttatccgac tccgaaggac caataatatt tgcaaatgct
     3541 catttttagg actttttttg aattcctgca gcccggggga tccactagtt ctagactcga
     3601 ggatctggtt accactaaac cagcctcaag aacacccgaa tggagtctct aagctacata
     3661 ataccaactt acactttaca aaatgttgtc ccccaaaatg tagccattcg tatctgctcc
     3721 taataaaaag aaagtttctt cacattctaa aaaaaaaaaa aaaaaaaaaa aaaacccccc
     3781 cccccccccc cggggncgcc accgnggggg agctccaatt cgccctatag tgagtcgtat
     3841 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     3901 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     3961 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggaa attgtaagcg
     4021 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     4081 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     4141 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     4201 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     4261 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     4321 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     4381 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     4441 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4501 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4561 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4621 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4681 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4741 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4801 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     4861 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     4921 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     4981 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5041 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5101 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5161 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5221 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5281 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5341 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5401 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5461 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5521 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5581 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5641 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5701 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5761 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5821 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5881 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5941 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6001 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6061 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6121 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6181 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6241 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6301 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6361 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     6421 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     6481 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     6541 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     6601 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     6661 aaaagctggg taccgggccc cccctcgagg tagcttcttg ttctttttgc agaagctcag
     6721 aataaacgct caactttggc agaatcgatc ccacc
//
