LOCUS       sequence-416637-        1325 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PLA2G15-6xHis S198A in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Nyame K, Hims A, Aburous A, Laqtom NN, Dong W, Medoh UN, Heiby JC, 
            Xiong J, Ori A, Abu-Remaileh M
  TITLE     Glycerophosphodiesters inhibit lysosomal phospholipid catabolism in 
            Batten disease.
  JOURNAL   Mol Cell. 2024 Apr 4;84(7):1354-1364.e9. doi: 
            10.1016/j.molcel.2024.02.006. Epub 2024 Mar 5.
  PUBMED    38447580
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.02.006"; journalName: "Mol Cell"; date: 
            "2024-04-4- 4"; volume: "84"; issue: "7"; pages: "1354-1364.e9"
COMMENT     Sequence Label: pRK5-PLA2G15-6xHis S198A
FEATURES             Location/Qualifiers
     source          1..1325
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1295..1312
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcccaggtcc aactgcacct cggttctatc gattgaattc accatgggcc tccacctccg
       61 cccctaccgt gtggggctgc tcccggatgg cctcctgttc ctcttgctgc tgctaatgct
      121 gctcgcggac ccagcgctcc cggccggacg tcacccccca gtggtgctgg tccctggtga
      181 tttgggtaac caactggaag ccaagctgga caagccgaca gtggtgcact acctctgctc
      241 caagaagacc gaaagctact tcacaatctg gctgaacctg gaactgctgc tgcctgtcat
      301 cattgactgc tggattgaca atatcaggct ggtttacaac aaaacatcca gggccaccca
      361 gtttcctgat ggtgtggatg tacgtgtccc tggctttggg aagaccttct cactggagtt
      421 cctggacccc agcaaaagca gcgtgggttc ctatttccac accatggtgg agagccttgt
      481 gggctggggc tacacacggg gtgaggatgt ccgaggggct ccctatgact ggcgccgagc
      541 cccaaatgaa aacgggccct acttcctggc cctccgcgag atgatcgagg agatgtacca
      601 gctgtatggg ggccccgtgg tgctggttgc ccacgctatg ggcaacatgt acacgctcta
      661 ctttctgcag cggcagccgc aggcctggaa ggacaagtat atccgggcct tcgtgtcact
      721 gggtgcgccc tgggggggcg tggccaagac cctgcgcgtc ctggcttcag gagacaacaa
      781 ccggatccca gtcatcgggc ccctgaagat ccgggagcag cagcggtcag ctgtctccac
      841 cagctggctg ctgccctaca actacacatg gtcacctgag aaggtgttcg tgcagacacc
      901 cacaatcaac tacacactgc gggactaccg caagttcttc caggacatcg gctttgaaga
      961 tggctggctc atgcggcagg acacagaagg gctggtggaa gccacgatgc cacctggcgt
     1021 gcagctgcac tgcctctatg gtactggcgt ccccacacca gactccttct actatgagag
     1081 cttccctgac cgtgacccta aaatctgctt tggtgacggc gatggtactg tgaacttgaa
     1141 gagtgccctg cagtgccagg cctggcagag ccgccaggag caccaagtgt tgctgcagga
     1201 gctgccaggc agcgagcaca tcgagatgct ggccaacgcc accaccctgg cctatctgaa
     1261 acgtgtgctc cttgggcccg gaggtggagg tagtcatcat caccatcacc actaggcggc
     1321 cgcta
//
