LOCUS       sequence-416651-       13718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To monitor the status of NRG1, the plasmid encodes a recombinant 
            NRG1 fused extracellularly to the mScarlet fluorescent protein and 
            intracellularly to the mNeonGreen fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13718)
  AUTHORS   Deguchi E, Lin S, Hirayama D, Matsuda K, Tanave A, Sumiyama K, 
            Tsukiji S, Otani T, Furuse M, Sorkin A, Matsuda M, Terai K
  TITLE     Low-affinity ligands of the epidermal growth factor receptor are 
            long-range signal transmitters in collective cell migration of 
            epithelial cells.
  JOURNAL   Cell Rep. 2024 Nov 14;43(11):114986. doi: 
            10.1016/j.celrep.2024.114986.
  PUBMED    39546398
REFERENCE   2  (bases 1 to 13718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-11-14"; volume: "43(11):114986. doi"; pages: " 
            10.1016/j.celrep.2024.114986"
COMMENT     Sequence Label: pCSIIpuro-NRG1-ScNeo
FEATURES             Location/Qualifiers
     source          1..13718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1676..1909
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2094..2138
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2412..2529
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2585..3747
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2816..3738
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3695..3715
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3772..4467
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(3905..3923)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4173..4192
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7227..7776
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7371..7388)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7598..7617
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7779..8378
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7779..7798)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8275..8295
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8413..9001
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8466..8486)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8884..8895)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9520..9700
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9726..9743)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9732..9956
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10002..10430
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10089..10108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10299..10320
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10439..10459)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10444..10773
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10624..10759
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10686..10705
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10821..10868
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10887..11261
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11391..11512
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11428..11447)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11482..11501
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11561..11577)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11561..11577)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11574..11596)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11585..11601
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11609..11615,11616..11633,11634..11639))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11654..11675
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11792..11809)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11963..12551)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12043..12062)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12722..13513,13514..13582))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13345..13364
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13583..13687)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gggccgctga tcttcagacc tggaggagga gatatgaggg
     1561 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1621 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1681 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1741 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1801 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1861 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1921 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1981 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2041 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2101 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2161 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     2221 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     2281 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     2341 gtggagagag agacagagac agatccattc gattagtgaa cggatctaca aatggcagta
     2401 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2461 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     2521 caaaattttc gggtttatta cagggacagc agaaattcac tttgaattaa ttcaagcttc
     2581 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     2641 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     2701 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     2761 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     2821 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     2881 aattacttcc acctggctcc agtacgtgat tcttgatccc gagctggagc caggggcggg
     2941 ccttgcgctt taggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
     3001 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
     3061 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
     3121 gtaaatgcgg gccaggatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     3181 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     3241 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     3301 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     3361 gaaagatggc cgcttcccgg ccctgctcca gggggctcaa aatggaggac gcggcgctcg
     3421 ggagagcggg cgggtgagtc acccacacaa aggaaagggg cctttccgtc ctcagccgtc
     3481 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctggagc
     3541 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     3601 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     3661 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     3721 ttttttcttc catttcaggt gtcgtgaaca cgctaccggt ccgaattcac catggtgagc
     3781 aagggcgagg cagtgatcaa ggagttcatg cggttcaagg tgcacatgga gggctccatg
     3841 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     3901 accgccaagc tgaaggtgac caagggtggc cccctgccct tctcctggga catcctgtcc
     3961 cctcagttca tgtacggctc cagggccttc accaagcacc ccgccgacat ccccgactac
     4021 tataagcagt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     4081 ggcgccgtga ccgtgaccca ggacacctcc ctggaggacg gcaccctgat ctacaaggtg
     4141 aagctccgcg gcaccaactt ccctcctgac ggccccgtaa tgcagaagaa gacaatgggc
     4201 tgggaagcgt ccaccgagcg gttgtacccc gaggacggcg tgctgaaggg cgacattaag
     4261 atggccctgc gcctgaagga cggaggccgc tacctggcgg acttcaagac cacctacaag
     4321 gccaagaagc ccgtgcagat gcccggcgcc tacaacgtcg accgcaagtt ggacatcacc
     4381 tcccacaacg aggactacac cgtggtggaa cagtacgaac gctccgaggg ccgccactcc
     4441 accggcggca tggacgagct gtacaaggga ggctcgagca tgagcgagcg caaagaaggc
     4501 agaggcaaag gcaagggaaa gaagaaagag cgcggcagcg gcaagaagcc tgaatctgct
     4561 gctggcagcc agtctcctgc tctgcctcct aggctgaaag agatgaagtc ccaagagagc
     4621 gccgctggct ctaagctggt gctgagatgc gaaaccagca gcgagtacag cagcctgagg
     4681 ttcaagtggt tcaagaacgg caacgagctg aaccggaaga acaagcccca gaacatcaag
     4741 atccagaaga agcccggcaa gagcgagctg aggatcaaca aagccagcct ggccgactcc
     4801 ggcgagtaca tgtgcaaagt gatcagcaag ctgggcaacg acagcgccag cgccaatatc
     4861 accatcgtgg aaagcaacga gatcatcacc ggcatgcctg cctctaccga aggcgcctat
     4921 gtgtctagcg agagccccat cagaatcagc gtgtccacag agggcgccaa caccagcagc
     4981 tctaccagca catctaccac cggcaccagc cacctggtca agtgcgccga gaaagaaaag
     5041 accttctgcg tgaatggcgg cgagtgcttc atggtcaagg acctgagcaa ccccagcaga
     5101 tacctgtgca agtgccccaa cgagttcacc ggcgacaggt gccagaatta cgtgatggcc
     5161 agcttctaca agcacctggg catcgagttc atggaagccg aggaactgta ccagaaaagg
     5221 gtgctgacca tcaccggaat ctgtatcgcc ctgctggtcg tgggcattat gtgcgtggtg
     5281 gcctactgca agaccaagaa gcagaggaag aagctgcacg acaggctgcg gcagtctctg
     5341 agatccgaga ggaacaacat gatgaatatc gccaacggac cccaccatcc taatcctcca
     5401 cctgagaacg tgcagctggt caaccagtac gtgtccaaga acgtgatcag ctccgagcac
     5461 atcgtcgaga gagaggccga gacaagcttc agcacctctc actacaccag cacagcccac
     5521 cacagcacca ccgtgacaca gaccccaagc cacagctggt ctaatggcca caccgagagc
     5581 atcctgagcg agtcccatag cgtgatcgtg atgagcagcg tggaaaacag caggcacagc
     5641 agccctacag gcggacctag aggcagactg aatggaaccg gcggacccag agagtgcaac
     5701 agctttctga ggcacgccag agagacaccc gacagctaca gagacagccc tcacagcgag
     5761 agatacgtgt ccgccatgac aacccctgcc agaatgagcc ccgtggactt tcacacccct
     5821 agcagcccaa aaagccctcc tagcgagatg agccctcctg tgtccagcat gaccgtgtcc
     5881 atgcctagca tggctgtgtc cccgtttatg gaagaggaaa ggcctctgct gctcgtgacc
     5941 cctccaagac tgagagagaa gaagttcgac caccatccac agcagttcag cagcttccat
     6001 cacaaccccg ctcacgacag caacagcctg cctgcttctc cactgaggat cgtcgaggac
     6061 gaggaatacg agacaaccca agagtacgag cccgctcaag agcccgtgaa gaaactggcc
     6121 aatagcaggc gggccaagag gaccaagcct aacggccaca ttgccaacag gctggaagtg
     6181 gactccaaca cctctagcca gagcagcaac tccgagagcg agacagagga tgagagagtg
     6241 ggcgaagata ccccattcct gggcattcag aatcctctgg ccgcctctct ggaagccaca
     6301 ccagctttta gactggccga cagcaggacc aatccagccg gaagattcag cacccaagag
     6361 gaaatccagg ccagactgtc ctccgtgatc gccaatcagg atcctatcgc cgtgggcgga
     6421 ttcgaaatgg taagcaaggg ggaggaagat aatatggctt cccttccagc aacgcacgaa
     6481 ttgcatatct ttggaagtat taatggagtt gatttcgaca tggtggggca gggtacggga
     6541 aacccaaacg atggctatga agaacttaac ctcaagtcta ccaaaggcga tctccaattc
     6601 tcaccctgga tattggtccc acacatcggc tacgggttcc atcaatacct gccttatcct
     6661 gacgggatga gtccttttca agctgctatg gttgacggaa gtggctacca agttcaccgc
     6721 acaatgcaat ttgaggatgg tgcatctctt acggttaatt accgatatac atatgagggt
     6781 tcacacatca agggggaagc tcaggtcaag ggcactggat ttcccgcaga tggcccagtt
     6841 atgaccaatt ctctgaccgc agccgattgg tgtcgaagca agaagaccta ccctaacgac
     6901 aagactatca tctcaacctt caaatggtcc tacaccacag gtaacggaaa gcggtatagg
     6961 tctacagctc ggacaacata tacctttgcg aagcctatgg ccgctaacta tttgaaaaac
     7021 caaccgatgt atgtttttcg gaagaccgaa ttgaaacatt caaaaacgga gttgaatttc
     7081 aaagagtggc agaaggcatt caccgacgta atgggtatgg atgagttgta taaataagcg
     7141 gcggccgcgg ctctagagga tccgttaact aacttaagct agcaacggtt tccctctagc
     7201 gggatcaatt ccgccccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     7261 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     7321 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     7381 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     7441 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     7501 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     7561 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     7621 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     7681 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     7741 cggggacgtg gttttccttt gaaaaacacg ataataccat gaccgagtac aagcccacgg
     7801 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     7861 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     7921 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     7981 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     8041 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     8101 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     8161 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     8221 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     8281 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     8341 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgact agagttaaca tcgagggatc
     8401 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     8461 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     8521 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     8581 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     8641 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     8701 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     8761 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     8821 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     8881 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     8941 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     9001 catcgatacc gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     9061 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     9121 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     9181 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     9241 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     9301 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     9361 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
     9421 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
     9481 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
     9541 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     9601 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     9661 tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg
     9721 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     9781 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     9841 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     9901 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     9961 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
    10021 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
    10081 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
    10141 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
    10201 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
    10261 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
    10321 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
    10381 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
    10441 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    10501 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
    10561 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
    10621 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
    10681 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
    10741 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
    10801 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat
    10861 aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca
    10921 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
    10981 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
    11041 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
    11101 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
    11161 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
    11221 ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg
    11281 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
    11341 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
    11401 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    11461 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    11521 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
    11581 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
    11641 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
    11701 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
    11761 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    11821 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
    11881 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    11941 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    12001 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    12061 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    12121 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    12181 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    12241 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    12301 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    12361 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
    12421 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    12481 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    12541 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    12601 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    12661 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    12721 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    12781 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    12841 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    12901 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    12961 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    13021 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    13081 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    13141 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    13201 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    13261 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    13321 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    13381 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    13441 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    13501 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    13561 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    13621 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    13681 ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
