LOCUS       sequence-416689-       10293 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To monitor the status of NRG1, the plasmid encodes a recombinant 
            NRG1 fused extracellularly to the mScarlet fluorescent protein and 
            intracellularly to the mNeonGreen fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10293)
  AUTHORS   Deguchi E, Lin S, Hirayama D, Matsuda K, Tanave A, Sumiyama K, 
            Tsukiji S, Otani T, Furuse M, Sorkin A, Matsuda M, Terai K
  TITLE     Low-affinity ligands of the epidermal growth factor receptor are 
            long-range signal transmitters in collective cell migration of 
            epithelial cells.
  JOURNAL   Cell Rep. 2024 Nov 14;43(11):114986. doi: 
            10.1016/j.celrep.2024.114986.
  PUBMED    39546398
REFERENCE   2  (bases 1 to 10293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-11-14"; volume: "43(11):114986. doi"; pages: " 
            10.1016/j.celrep.2024.114986"
COMMENT     Sequence Label: pPBpuro-NRG1-ScNeo
FEATURES             Location/Qualifiers
     source          1..10293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1392..1666
                     /label=chicken beta-actin promoter
     intron          1667..2683
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2607..2629
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2691..2710
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2753..3448
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(2886..2904)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3154..3173
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6199..6748
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6343..6360)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6570..6589
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6751..7350
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6751..6770)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7247..7267
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7377..7394)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7383..7607
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7654..7670)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7678..7694
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7702..7708,7709..7726,7727..7732))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7747..7768
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   8002..8064
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8105..8125)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8105..8123)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8144..8160)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8168..8184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8192..8198,8199..8216,8217..8222))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8237..8258
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8375..8392)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8546..9134)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8626..8645)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9305..10096,10097..10165))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9928..9947
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10166..10270)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg
     1561 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
     1621 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
     1681 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     1741 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
     1801 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
     1861 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
     1921 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc
     1981 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg
     2041 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg
     2101 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt
     2161 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc
     2221 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc
     2281 cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc
     2341 ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt
     2401 gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg
     2461 cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc
     2521 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg
     2581 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
     2641 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     2701 ggttattgtg ctgtctcatc attttggcaa agaattggga tccgaattca ccatggtgag
     2761 caagggcgag gcagtgatca aggagttcat gcggttcaag gtgcacatgg agggctccat
     2821 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     2881 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttctcctggg acatcctgtc
     2941 ccctcagttc atgtacggct ccagggcctt caccaagcac cccgccgaca tccccgacta
     3001 ctataagcag tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     3061 cggcgccgtg accgtgaccc aggacacctc cctggaggac ggcaccctga tctacaaggt
     3121 gaagctccgc ggcaccaact tccctcctga cggccccgta atgcagaaga agacaatggg
     3181 ctgggaagcg tccaccgagc ggttgtaccc cgaggacggc gtgctgaagg gcgacattaa
     3241 gatggccctg cgcctgaagg acggaggccg ctacctggcg gacttcaaga ccacctacaa
     3301 ggccaagaag cccgtgcaga tgcccggcgc ctacaacgtc gaccgcaagt tggacatcac
     3361 ctcccacaac gaggactaca ccgtggtgga acagtacgaa cgctccgagg gccgccactc
     3421 caccggcggc atggacgagc tgtacaaggg aggctcgagc atgagcgagc gcaaagaagg
     3481 cagaggcaaa ggcaagggaa agaagaaaga gcgcggcagc ggcaagaagc ctgaatctgc
     3541 tgctggcagc cagtctcctg ctctgcctcc taggctgaaa gagatgaagt cccaagagag
     3601 cgccgctggc tctaagctgg tgctgagatg cgaaaccagc agcgagtaca gcagcctgag
     3661 gttcaagtgg ttcaagaacg gcaacgagct gaaccggaag aacaagcccc agaacatcaa
     3721 gatccagaag aagcccggca agagcgagct gaggatcaac aaagccagcc tggccgactc
     3781 cggcgagtac atgtgcaaag tgatcagcaa gctgggcaac gacagcgcca gcgccaatat
     3841 caccatcgtg gaaagcaacg agatcatcac cggcatgcct gcctctaccg aaggcgccta
     3901 tgtgtctagc gagagcccca tcagaatcag cgtgtccaca gagggcgcca acaccagcag
     3961 ctctaccagc acatctacca ccggcaccag ccacctggtc aagtgcgccg agaaagaaaa
     4021 gaccttctgc gtgaatggcg gcgagtgctt catggtcaag gacctgagca accccagcag
     4081 atacctgtgc aagtgcccca acgagttcac cggcgacagg tgccagaatt acgtgatggc
     4141 cagcttctac aagcacctgg gcatcgagtt catggaagcc gaggaactgt accagaaaag
     4201 ggtgctgacc atcaccggaa tctgtatcgc cctgctggtc gtgggcatta tgtgcgtggt
     4261 ggcctactgc aagaccaaga agcagaggaa gaagctgcac gacaggctgc ggcagtctct
     4321 gagatccgag aggaacaaca tgatgaatat cgccaacgga ccccaccatc ctaatcctcc
     4381 acctgagaac gtgcagctgg tcaaccagta cgtgtccaag aacgtgatca gctccgagca
     4441 catcgtcgag agagaggccg agacaagctt cagcacctct cactacacca gcacagccca
     4501 ccacagcacc accgtgacac agaccccaag ccacagctgg tctaatggcc acaccgagag
     4561 catcctgagc gagtcccata gcgtgatcgt gatgagcagc gtggaaaaca gcaggcacag
     4621 cagccctaca ggcggaccta gaggcagact gaatggaacc ggcggaccca gagagtgcaa
     4681 cagctttctg aggcacgcca gagagacacc cgacagctac agagacagcc ctcacagcga
     4741 gagatacgtg tccgccatga caacccctgc cagaatgagc cccgtggact ttcacacccc
     4801 tagcagccca aaaagccctc ctagcgagat gagccctcct gtgtccagca tgaccgtgtc
     4861 catgcctagc atggctgtgt ccccgtttat ggaagaggaa aggcctctgc tgctcgtgac
     4921 ccctccaaga ctgagagaga agaagttcga ccaccatcca cagcagttca gcagcttcca
     4981 tcacaacccc gctcacgaca gcaacagcct gcctgcttct ccactgagga tcgtcgagga
     5041 cgaggaatac gagacaaccc aagagtacga gcccgctcaa gagcccgtga agaaactggc
     5101 caatagcagg cgggccaaga ggaccaagcc taacggccac attgccaaca ggctggaagt
     5161 ggactccaac acctctagcc agagcagcaa ctccgagagc gagacagagg atgagagagt
     5221 gggcgaagat accccattcc tgggcattca gaatcctctg gccgcctctc tggaagccac
     5281 accagctttt agactggccg acagcaggac caatccagcc ggaagattca gcacccaaga
     5341 ggaaatccag gccagactgt cctccgtgat cgccaatcag gatcctatcg ccgtgggcgg
     5401 attcgaaatg gtaagcaagg gggaggaaga taatatggct tcccttccag caacgcacga
     5461 attgcatatc tttggaagta ttaatggagt tgatttcgac atggtggggc agggtacggg
     5521 aaacccaaac gatggctatg aagaacttaa cctcaagtct accaaaggcg atctccaatt
     5581 ctcaccctgg atattggtcc cacacatcgg ctacgggttc catcaatacc tgccttatcc
     5641 tgacgggatg agtccttttc aagctgctat ggttgacgga agtggctacc aagttcaccg
     5701 cacaatgcaa tttgaggatg gtgcatctct tacggttaat taccgatata catatgaggg
     5761 ttcacacatc aagggggaag ctcaggtcaa gggcactgga tttcccgcag atggcccagt
     5821 tatgaccaat tctctgaccg cagccgattg gtgtcgaagc aagaagacct accctaacga
     5881 caagactatc atctcaacct tcaaatggtc ctacaccaca ggtaacggaa agcggtatag
     5941 gtctacagct cggacaacat atacctttgc gaagcctatg gccgctaact atttgaaaaa
     6001 ccaaccgatg tatgtttttc ggaagaccga attgaaacat tcaaaaacgg agttgaattt
     6061 caaagagtgg cagaaggcat tcaccgacgt aatgggtatg gatgagttgt ataaataagc
     6121 ggcggccgct ctagagtcaa ctaacttaag ctagcaacgg tttccctcta gcgggatcaa
     6181 ttccgccccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     6241 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     6301 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     6361 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     6421 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     6481 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     6541 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     6601 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     6661 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     6721 tggttttcct ttgaaaaaca cgataatacc atgaccgagt acaagcccac ggtgcgcctc
     6781 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     6841 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     6901 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     6961 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     7021 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     7081 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     7141 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     7201 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     7261 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     7321 tggtgcatga cccgcaagcc cggtgcctga tcgatgatct agagctcgct gatcagcctc
     7381 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     7441 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     7501 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     7561 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     7621 aagaacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     7681 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7741 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     7801 gggaaacctg tcgtgccagc ggatcttaaa agttttgtta ctttatagaa gaaattttga
     7861 gtttttgttt ttttttaata aataaataaa cataaataaa ttgtttgttg aatttattat
     7921 tagtatgtaa gtgtaaatat aataaaactt aatatctatt caaattaata aataaacctc
     7981 gatatacaga ccgataaaac acatgcgtca attttacgca tgattatctt taacgtacgt
     8041 cacaatatga ttatctttct agggttaatc tagtatacgc gtttaattaa tccagctttt
     8101 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     8161 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     8221 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     8281 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     8341 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8401 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8461 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8521 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8581 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8641 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8701 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8761 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8821 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8881 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8941 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     9001 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9061 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9121 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9181 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     9241 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     9301 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     9361 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     9421 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     9481 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9541 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9601 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9661 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9721 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9781 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9841 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9901 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9961 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    10021 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    10081 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    10141 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    10201 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    10261 gggttccgcg cacatttccc cgaaaagtgc cac
//
