LOCUS       sequence-416901-        9249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral construct expressing FLAG Metap2 and shRNA against 
            Luciferase (control shRNA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9249)
  AUTHORS   Shin HR, Citron YR, Wang L, Tribouillard L, Goul CS, Stipp R, 
            Sugasawa Y, Jain A, Samson N, Lim CY, Davis OB, Castaneda-Carpio D, 
            Qian M, Nomura DK, Perera RM, Park E, Covey DF, Laplante M, Evers 
            AS, Zoncu R
  TITLE     Lysosomal GPCR-like protein LYCHOS signals cholesterol sufficiency 
            to mTORC1.
  JOURNAL   Science. 2022 Sep 16;377(6612):1290-1298. doi: 
            10.1126/science.abg6621. Epub 2022 Aug 25.
  PUBMED    36007018
REFERENCE   2  (bases 1 to 9249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abg6621"; journalName: "Science"; date: 
            "2022-09-16- 16"; volume: "377"; issue: "6612"; pages: "1290-1298"
COMMENT     Sequence Label: pLKO shLuc/FLAG Metap2
FEATURES             Location/Qualifiers
     source          1..9249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..514
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     433..451
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      523..532
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             532..555
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2107..2680
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2274..2291)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2501..2520
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2700..3299
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2700..2719)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3196..3216
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3427..3660
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3732..3853
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3769..3788)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3823..3842
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3893..4028
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4048..4067)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4049..4067)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4077..4094)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4077..4093)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4086..4108)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4235..4690
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4322..4341)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4532..4553
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4716..4820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4821..4889,4890..5681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5039..5058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5852..6440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6341..6360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6594..6611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6728..6749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6764..6769,6770..6787,6788..6794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6802..6818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6807..6829
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6826..6842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6826..6842
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6861..6881
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6863..6881
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6909..7135
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7136..7316
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7363..7488
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7981..8214
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8399..8443
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8741..8989
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8741..8761
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8912..8931
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    9087..9204
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gagggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg
       61 ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac
      121 gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct
      181 tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca
      241 aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc
      301 agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag
      361 cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt
      421 cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc
      481 cctcgttgac cgaatcaccg acctctctcc ccagggggat ccgccaccat ggactacaag
      541 gatgacgatg acaagggtgg tggtggtggt gcgtcgacga tggcgggtgt ggaggaggta
      601 gcggcctccg ggagccacct gaatggcgac ctggatccag acgacaggga agaaggagct
      661 gcctctacgg ctgaggaagc agccaagaaa aaaagacgaa agaagaagaa gagcaaaggg
      721 ccttctgcag gggaacagga acctgataaa gaatcaggag cctcagtgga tgaagtagca
      781 agacagttgg aaagatcagc attggaagat aaagaaagag atgaagatga tgaagatgga
      841 gatggcgatg gagatggagc aactggaaag aagaagaaaa agaagaagaa gaagagagga
      901 ccaaaagttc aaacagaccc tccctcagtt ccaatatgtg acctgtatcc taatggtgta
      961 tttcccaaag gacaagaatg cgaataccca cccacacaag atgggcgaac agctgcttgg
     1021 agaactacaa gtgaagaaaa gaaagcatta gatcaggcaa gtgaagagat ttggaatgat
     1081 tttcgagaag ctgcagaagc acatcgacaa gttagaaaat acgtaatgag ctggatcaag
     1141 cctgggatga caatgataga aatctgtgaa aagttggaag actgttcacg caagttaata
     1201 aaagagaatg gattaaatgc aggcctggca tttcctactg gatgttctct caataattgt
     1261 gctgcccatt atactcccaa tgccggtgac acaacagtat tacagtatga tgacatctgt
     1321 aaaatagact ttggaacaca tataagtggt aggattattg actgtgcttt tactgtcact
     1381 tttaatccca aatatgatac gttattaaaa gctgtaaaag atgctactaa cactggaata
     1441 aagtgtgctg gaattgatgt tcgtctgtgt gatgttggtg aggccatcca agaagttatg
     1501 gagtcctatg aagttgaaat agatgggaag acatatcaag tgaaaccaat ccgtaatcta
     1561 aatggacatt caattgggca atatagaata catgctggaa aaacagtgcc gattgtgaaa
     1621 ggaggggagg caacaagaat ggaggaagga gaagtatatg caattgaaac ctttggtagt
     1681 acaggaaaag gtgttgttca tgatgatatg gaatgttcac attacatgaa aaattttgat
     1741 gttggacatg tgccaataag gcttccaaga acaaaacact tgttaaatgt catcaatgaa
     1801 aactttggaa cccttgcctt ctgccgcaga tggctggatc gcttgggaga aagtaaatac
     1861 ttgatggctc tgaagaatct gtgtgacttg ggcattgtag atccatatcc accattatgt
     1921 gacattaaag gatcatatac agcgcaattt gaacatacca tcctgttgcg tccaacatgt
     1981 aaagaagttg tcagcagagg agatgactat taatctagaa tcagataatg ggaacattat
     2041 gatccagaaa cacgatggca tcacggtggc agtgcacaaa atggcctctt gatctagacc
     2101 cgggcgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     2161 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     2221 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     2281 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     2341 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     2401 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     2461 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     2521 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     2581 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     2641 ccacggggac gtggttttcc tttgaaaaac acgatgataa agatccaccg gagcttacca
     2701 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     2761 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     2821 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     2881 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     2941 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     3001 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     3061 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     3121 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     3181 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     3241 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
     3301 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcggtac
     3361 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     3421 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     3481 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     3541 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     3601 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     3661 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     3721 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3781 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3841 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     3901 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     3961 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     4021 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     4081 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     4141 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     4201 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     4261 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4321 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4381 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4441 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4501 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4561 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4621 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     4681 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5581 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     6001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     6661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     6721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     6781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     6841 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     6901 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     6961 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     7021 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     7081 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     7141 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7201 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7261 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     7321 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     7381 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     7441 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     7501 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     7561 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     7621 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     7681 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     7741 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     7801 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     7861 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     7921 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     7981 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     8041 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     8101 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     8161 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     8221 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     8281 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     8341 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     8401 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     8461 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     8521 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     8581 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     8641 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     8701 tcgatcacga gactagcctc gagcggccgc ccccttcacc gagggcctat ttcccatgat
     8761 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
     8821 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
     8881 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
     8941 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg gtcctaaggt
     9001 taagtcgccc tcgctcgagc gagggcgact taaccttagg tttttgaatt ctcgacctcg
     9061 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     9121 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     9181 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
     9241 ccgcggctc
//
