LOCUS       sequence-417304-        3575 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Gilbert C, Lee H
  TITLE     POSSUM Expansion Pack
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pGL0_157 [catP]
FEATURES             Location/Qualifiers
     source          1..3575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1322..1338)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1322..1338)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1335..1357)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1346..1362
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1370..1376,1377..1394,1395..1400))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1415..1436
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1553..1570)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1724..2312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1804..1823)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2483..3274,3275..3343))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3106..3125
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3344..3448)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3516..3534
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccc tagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accaaatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcatcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgcaac gcgatgacga tggatagcga
      421 ttcatcgatg agctgacccg atcgccgccg ccggagggtt gcgtttgaga caggcgacag
      481 attagcgtgc gaacagcacg ctattgttgt atcattactc gcatccattc tcaggctgtc
      541 tcgtctcgtc tccgaggtct caaatggtat ttgaaaaaat tgataaaaat agttggaaca
      601 gaaaagagta ttttgaccac tactttgcaa gtgtaccttg tacctacagc atgaccgtta
      661 aagtggatat cacacaaata aaggaaaagg gaatgaaact atatcctgca atgctttatt
      721 atattgcaat gattgtaaac cgccattcag agtttaggac ggcaatcaat caagatggtg
      781 aattggggat atatgatgag atgataccaa gctatacaat atttcacaat gatactgaaa
      841 cattttccag cctttggact gagtgtaagt ctgactttaa atcattttta gcagattatg
      901 aaagtgatac gcaacggtat ggaaacaatc atagaatgga aggaaagcca aatgctccgg
      961 aaaacatttt taatgtatct atgataccgt ggtcaacctt cgatggcttt aatctgaatt
     1021 tgcagaaagg atatgattat ttgattccta tttttactat ggggaaatat tataaagaag
     1081 ataacaaaat tatacttcct ttggcaattc aagttcatca cgcagtatgt gacggatttc
     1141 acatttgccg ttttgtaaac gaattgcagg aattgataaa tagttaagct ttgagaccac
     1201 cccaggatac atagattacc acaactccga gcccttccac ctagcgtgct aaccacgcac
     1261 gctattgttg tatatcagtt ctggaccagc gagctgtgct gcgactcgtg gcgtaatcat
     1321 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     1381 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     1441 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     1501 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     1561 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1621 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1681 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1741 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1801 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1861 tgtcgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1921 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1981 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2041 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2101 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2161 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2221 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2281 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2341 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2401 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2461 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2521 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2581 gggagggctt accatctggc cccagtgctg caatgatacc gcgagatcca cgctcaccgg
     2641 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2701 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2761 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2821 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2881 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2941 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3001 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3061 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3121 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3181 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3241 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3301 caaaaaaggg aataagggcg acacggaaat gttgaatact catactctac ctttttcaat
     3361 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3421 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     3481 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     3541 atctcgcgcg tttcggtgca cgaggccctt tcatc
//
