LOCUS       sequence-417396-       10026 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multiplexed gRNA plasmid targeting six different gene promoters 
            (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1), with mCherry 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10026)
  AUTHORS   Swain T, Pflueger C, Freytag S, Poppe D, Pflueger J, Nguyen TV, Li 
            JK, Lister R
  TITLE     A modular dCas9-based recruitment platform for combinatorial 
            epigenome editing.
  JOURNAL   Nucleic Acids Res. 2023 Nov 24:gkad1108. doi: 10.1093/nar/gkad1108.
  PUBMED    38000387
REFERENCE   2  (bases 1 to 10026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Nov 24:gkad1108. doi: 10.1093/nar/gkad1108."
COMMENT     Sequence Label: LLP773_pLenti-6xHRBKET-sgRNA
FEATURES             Location/Qualifiers
     source          1..10026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2119..2322
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2273..2293
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2324..2342
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2345..2363
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             2371..3081
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2516..2534)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2784..2803
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     intron          3120..3349
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     3332..3351
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    3528..3649
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3739..3987
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        4008..4083
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        4190..4438
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        4459..4534
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        4661..4909
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        4929..5004
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        5145..5393
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        5413..5488
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        5603..5851
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        5871..5946
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        6076..6324
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        6343..6418
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     LTR             6547..6780
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6852..6973
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7013..7148
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7168..7187)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7169..7187)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7197..7214)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7197..7213)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7206..7228)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7355..7810
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7442..7461)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7652..7673
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7836..7940
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7941..8009,8010..8801)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8159..8178)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8972..9560
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9461..9480
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9714..9731
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9848..9869
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9884..9889,9890..9907,9908..9914)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9922..9938
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9927..9949
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9946..9962
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9946..9962
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9981..10001
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9983..10001
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     1801 agcttgggag ttccgcttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     1861 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     1921 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     1981 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     2041 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     2101 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     2161 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     2221 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     2281 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctccctat cagtgataga
     2341 gatctcccta tcagtgatag agatcgtcga atggtgagca agggcgagga ggataacatg
     2401 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     2461 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     2521 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     2581 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     2641 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     2701 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     2761 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     2821 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     2881 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     2941 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     3001 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     3061 atggacgagc tgtacaagtg atggaattaa ttcgctgtct gcgagggcca gctgttgggg
     3121 tgagtactcc ctctcaaaag cgggcatgac ttctgcgcta agattgtcag tttccaaaaa
     3181 cgaggaggat ttgatattca cctggcccgc ggtgatgcct ttgagggtgg ccgcgtccat
     3241 ctggtcagaa aagacaatct ttttgttgtc aagcttgagg tgtggcaggc ttgagatctg
     3301 gccatacact tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc
     3361 caggtccaac tgcaggtcga tttaaatggg aattccacag cggccgcatg tctagataac
     3421 tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     3481 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     3541 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3601 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa tgcgtcgagt
     3661 gcctcgaggt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac tggatccggt
     3721 accaaggtcg ggcaggaaga gggcctattt cccatgattc cttcatattt gcatatacga
     3781 tacaaggctg ttagagagat aattagaatt aatttgactg taaacacaaa gatattagta
     3841 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     3901 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
     3961 tatatcttgt ggaaaggacg aaacaccgac caggaacttc cccaagcgtt ttagagctag
     4021 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
     4081 tgcttttttt accgtcgacc tctagctaga gcttggcggg tgtacaaaaa agcaggcttt
     4141 aaaggaacca attcagtcga ctggatccgg taccaaggtc gggcaggaag agggcctatt
     4201 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat
     4261 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
     4321 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
     4381 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccga
     4441 agacatccga tccagcgggt tttagagcta gaaatagcaa gttaaaataa ggctagtccg
     4501 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt taccggttgt ggtttgtcca
     4561 aactcatcaa tgtatcttat catgtctgta ttgtacaaaa aagcaggctt taaaggaacc
     4621 aattcagtcg actggatccg gtaccaaggt cgggcaggaa gagggcctat ttcccatgat
     4681 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac
     4741 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
     4801 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
     4861 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg ggtaggagag
     4921 actcacgcgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
     4981 gaaaaagtgg caccgagtcg gtgctttttt taccgtcgac tgcattctag ttgtggtttg
     5041 tccaaactca tcaatgtatc ttatcatgtc tgtattgtac aaaaaagcag gctttaaagg
     5101 aaccaattca gtcgactgga tccggtacca aggtcgggca ggaagagggc ctatttccca
     5161 tgattccttc atatttgcat atacgataca aggctgttag agagataatt agaattaatt
     5221 tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg
     5281 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
     5341 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac accggaggca
     5401 gtcccggctc gcgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     5461 acttgaaaaa gtggcaccga gtcggtgctt tttttaccgt cgacctctag ctagtatctt
     5521 atcatgtctg tattgtacaa aaaagcaggc tttaaaggaa ccaattcagt cgactggatc
     5581 cggtaccaag gtcgggcagg aagagggcct atttcccatg attccttcat atttgcatat
     5641 acgatacaag gctgttagag agataattag aattaatttg actgtaaaca caaagatatt
     5701 agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt
     5761 atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc
     5821 tttatatatc ttgtggaaag gacgaaacac cgggagttgg gggagtgagt gttttagagc
     5881 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     5941 cggtgctttt tttaccgtcg acctctagct agagaaactc atcaatgtat cttatcatgt
     6001 ctgtattgta caaaaaagca ggctttaaag gaaccaattc agtcgactgg atccggtacc
     6061 aaggtcgggc aggaagaggg cctatttccc atgattcctt catatttgca tatacgatac
     6121 aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa
     6181 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
     6241 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
     6301 atcttgtgga aaggacgaaa caccggacct accggctccg cggttttaga gctagaaata
     6361 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
     6421 tttttaccgt cgacctctag ctagagcttg gcgtaatcga gcttggcgta atcatggtac
     6481 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     6541 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     6601 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6661 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6721 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6781 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     6841 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6901 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6961 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     7021 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7081 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7141 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     7201 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     7261 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     7321 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     7381 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     7441 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     7501 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     7561 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     7621 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     7681 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     7741 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     7801 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7861 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7921 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7981 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     8041 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     8101 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     8161 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     8221 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     8281 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     8341 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     8401 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     8461 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     8521 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     8581 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     8641 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     8701 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     8761 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8821 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8881 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8941 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     9001 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     9061 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     9121 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9181 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9241 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     9301 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     9361 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     9421 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     9481 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     9541 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     9601 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     9661 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     9721 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     9781 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     9841 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     9901 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     9961 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
    10021 aagctt
//
