LOCUS       sequence-417450-        8260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 empty backbone for strain-specific barcode DNA tag through 
            Tn7 bacterial insertion, Gentamicin selectable marker and GFP 
            fluorescent marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   Ordon J, Thouin J, Nakano RT, Ma KW, Zhang P, Huettel B, 
            Garrido-Oter R, Schulze-Lefert P
  TITLE     Chromosomal barcodes for simultaneous tracking of near-isogenic 
            bacterial strains in plant microbiota.
  JOURNAL   Nat Microbiol. 2024 Apr;9(4):1117-1129. doi: 
            10.1038/s41564-024-01619-8. Epub 2024 Mar 19.
  PUBMED    38503974
REFERENCE   2  (bases 1 to 8260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-024-01619-8"; journalName: "Nat Microbiol"; date: 
            "2024-04"; volume: "9"; issue: "4"; pages: "1117-1129"
COMMENT     Sequence Label: pBCC090
FEATURES             Location/Qualifiers
     source          1..8260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      308..402
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      504..590
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    complement(623..670)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(711..716,717..733,734..739)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             928..1461
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     protein_bind    complement(1591..1638)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(1666..1671,1672..1687,1688..1694)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1702..1718
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1741..2454
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSVMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1771..1799)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2394..2414
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      2477..2571
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        join(2720..2725,2726..2743,2744..2750)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2804..3463
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2870..2889)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3805..4110
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3989..4008
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     mobile_element  4264..4429
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        4472..4917
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(4918..5004,5005..6339)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     terminator      6422..6516
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(6637..6656)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6648..7463
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            7630..7738
                     /note="incP origin of transfer"
     rep_origin      7749..8137
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      complement(8168..8254)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 tgccgaattc ggatccggag ggagtgtggg cggacaataa agtcttaaac tgaacaaaat
      121 agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag
      181 tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat
      241 tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggcgctgca agaattcaag
      301 cttggaggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      361 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcta gactgcgatg
      421 agtggcaggg cggggcgtaa tttcttaagg cagttattgg tgcccttaaa cgcctggggt
      481 aatgactctc tagcttgagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      541 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgcccgccg
      601 ctgcaagaat tcaagcttgg aggaagttcc tatactttct agagaatagg aacttcggaa
      661 taggaacttc aagatcccct gattcccttt gtcaacagca atggatcgaa ttgacataag
      721 cctgttcggt tcgtaaactg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
      781 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
      841 gtttttttgt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
      901 gatgtttgat gttatggagc agcaacgatg ttacgcagca gcaacgatgt tacgcagcag
      961 ggcagtcgcc ctaaaacaaa gttaggtggc tcaagtatgg gcatcattcg cacatgtagg
     1021 ctcggccctg accaagtcaa atccatgcgg gctgctcttg atcttttcgg tcgtgagttc
     1081 ggagacgtag ccacctactc ccaacatcag ccggactccg attacctcgg gaacttgctc
     1141 cgtagtaaga cattcatcgc gcttgctgcc ttcgaccaag aagcggttgt tggcgctctc
     1201 gcggcttacg ttctgcccaa gtttgagcag ccgcgtagtg agatctatat ctatgatctc
     1261 gcagtctccg gcgagcaccg gaggcagggc attgccaccg cgctcatcaa tctcctcaag
     1321 catgaggcca acgcgcttgg tgcttatgtg atctacgtgc aagcagatta cggtgacgat
     1381 cccgcagtgg ctctctatac aaagttgggc atacgggaag aagtgatgca ctttgatatc
     1441 gacccaagta ccgccaccta acaattcgtt caagccgaga tcggcttccc ggccgcggag
     1501 ttgttcggta aattgtcaca acgccgcggc caattcgatc tagaattcca ttgagtaagt
     1561 ttttaagcac atcagcttca aaagcgctct gaagttccta tactttctag agaataggaa
     1621 cttcggaata ggtacttccg ctactagaat tcgagcgggg aattcttgac aattaatcat
     1681 cggctcgtat aatgtgtgga attgtgagcg gataacaatt ttcacacagg aaacagctaa
     1741 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
     1801 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
     1861 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
     1921 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
     1981 catgactttt tcaagagtgt catgcccgaa ggttatgtac aggaaagaac tatatttttc
     2041 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
     2101 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
     2161 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
     2221 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
     2281 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     2341 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
     2401 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaatgagct
     2461 taattagctg agcttggact cctgttgata gatccagtaa tgacctcaga actccatctg
     2521 gatttgttca gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tctcccgctt
     2581 tacgaattcc catggggagc atgcggagtt acgtcatccc caccttccgc tgcgttcttc
     2641 atcgatgccc gcttagtgat atgcttaaaa ctccaagcac agccttctcc ccggggcggc
     2701 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     2761 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     2821 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     2881 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     2941 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     3001 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     3061 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     3121 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     3181 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     3241 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     3301 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     3361 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     3421 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg gatccggctt
     3481 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     3541 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     3601 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     3661 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     3721 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     3781 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     3841 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg acgacggatg
     3901 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     3961 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     4021 gtatccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     4081 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagcccccgg
     4141 ggatgctggt tgtcattgcc cctccttcct ctaagtgata aggttacttc ctctaaatga
     4201 ccaagcaggg tatctaatcc tgttaacgct cgctcagaga attcgcatgc ggagtcgagg
     4261 gagaaccaga taagtgaaat ctagttccaa actattttgt catttttaat tttcgtatta
     4321 gcttacgacg ctacacccag ttcccatcta ttttgtcact cttccctaaa taatccttaa
     4381 aaactccatt tccacccctc ccagttccca actattttgt ccgcccacac gctgagcgaa
     4441 ttccacgctt gtggaattcc tcgagggagt tcacatatac ctgccgttca ctattattta
     4501 gtgaaatgag atattatgat attttctgaa ttgtgattaa aaaggcaact ttatgcccat
     4561 gcaacagaaa ctataaaaaa tacagagaat gaaaagaaac agatagattt tttagttctt
     4621 taggcccgta gtctgcaaat ccttttatga ttttctatca aacaaaagag gaaaatagac
     4681 cagttgcaat ccaaacgaga gtctaataga atgaggtcga aaagtaaatc gcgcgggttt
     4741 gttactgata aagcaggcaa gacctaaaat gtgtaaaggg caaagtgtat actttggcgt
     4801 caccccttac atattttagg tcttttttta ttgtgcgtaa ctaacttgcc atcttcaaac
     4861 aggagggctg gaagaagcag accgctaaca cagtacataa aaaaggagac atgaacgatg
     4921 aacatcaaaa agtttgcaaa acaagcaaca gtattaacct ttactaccgc actgctggca
     4981 ggaggcgcaa ctcaagcgtt tgcgaaagaa acgaaccaaa agccatataa ggaaacatac
     5041 ggcatttccc atattacacg ccatgatatg ctgcaaatcc ctgaacagca aaaaaatgaa
     5101 aaatatcaag ttcctgaatt cgattcgtcc acaattaaaa atatctcttc tgcaaaaggc
     5161 ctggacgttt gggacagctg gccattacaa aacgctgacg gcactgtcgc aaactatcac
     5221 ggctaccaca tcgtctttgc attagccgga gatcctaaaa atgcggatga cacatcgatt
     5281 tacatgttct atcaaaaagt cggcgaaact tctattgaca gctggaaaaa cgctggccgc
     5341 gtctttaaag acagcgacaa attcgatgca aatgattcta tcctaaaaga ccaaacacaa
     5401 gaatggtcag gttcagccac atttacatct gacggaaaaa tccgtttatt ctacactgat
     5461 ttctccggta aacattacgg caaacaaaca ctgacaactg cacaagttaa cgtatcagca
     5521 tcagacagct ctttgaacat caacggtgta gaggattata aatcaatctt tgacggtgac
     5581 ggaaaaacgt atcaaaatgt acagcagttc atcgatgaag gcaactacag ctcaggcgac
     5641 aaccatacgc tgagagatcc tcactacgta gaagataaag gccacaaata cttagtattt
     5701 gaagcaaaca ctggaactga agatggctac caaggcgaag aatctttatt taacaaagca
     5761 tactatggca aaagcacatc attcttccgt caagaaagtc aaaaacttct gcaaagcgat
     5821 aaaaaacgca cggctgagtt agcaaacggc gctctcggta tgattgagct aaacgatgat
     5881 tacacactga aaaaagtgat gaaaccgctg attgcatcta acacagtaac agatgaaatt
     5941 gaacgcgcga acgtctttaa aatgaacggc aaatggtacc tgttcactga ctcccgcgga
     6001 tcaaaaatga cgattgacgg cattacgtct aacgatattt acatgcttgg ttatgtttct
     6061 aattctttaa ctggcccata caagccgctg aacaaaactg gccttgtgtt aaaaatggat
     6121 cttgatccta acgatgtaac ctttacttac tcacacttcg ctgtacctca agcgaaagga
     6181 aacaatgtcg tgattacaag ctatatgaca aacagaggat tctacgcaga caaacaatca
     6241 acgtttgcgc caagcttcct gctgaacatc aaaggcaaga aaacatctgt tgtcaaagac
     6301 agcatccttg aacaaggaca attaacagtt aacaaataaa ttcgctgagc gaggatgcac
     6361 atgtgaccga gggaagcttg cggccgcgtc gtgactggga aaaccctggc gactagtctt
     6421 ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg
     6481 ctcggttgcc gccgggcgtt ttttattggt gagaatccag gggtccccaa taattacgat
     6541 ttaaatttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     6601 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
     6661 agcgtgaaac gagctgtagc cgtccgcgtc tgaacagcaa catggatgcg gatctgtatg
     6721 gctataaatg ggcgcgtgat aacgtgggtc agagcggcgc gaccatttat cgtctgtatg
     6781 gcaaaccgga tgcgccggaa ctgtttctga aacatggcaa aggcagcgtg gcgaacgatg
     6841 tgaccgatga aatggtgcgt ctgaactggc tgaccgaatt tatgccgctg ccgaccatta
     6901 aacattttat tcgcaccccg gatgatgcgt ggctgctgac caccgcgatt ccgggcaaaa
     6961 ccgcgtttca ggtgctggaa gaatatccgg atagcggcga aaacattgtg gatgcgctgg
     7021 ccgtgtttct gcgtcgtctg catagcattc cggtgtgcaa ctgcccgttt aacagcgatc
     7081 gtgtgtttcg tctggcccag gcgcagagcc gtatgaacaa cggcctggtg gatgcgagcg
     7141 attttgatga tgaacgtaac ggctggccgg tggaacaggt gtggaaagaa atgcataaac
     7201 tgctgccgtt tagcccggat agcgtggtga cccacggcga ttttagcctg gataacctga
     7261 ttttcgatga aggcaaactg attggctgca ttgatgtggg ccgtgtgggc attgcggatc
     7321 gttatcagga tctggccatt ctgtggaact gcctgggcga atttagcccg agcctgcaaa
     7381 aacgtctgtt tcagaaatat ggcattgata atccggatat gaacaaactg caatttcatc
     7441 tgatgctgga tgaatttttc taataattaa ttggaccgcg gtccgcgcgt tgtccttttc
     7501 cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt
     7561 cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg
     7621 gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac
     7681 tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccgta
     7741 ggccggccga tctgaagatc agcagttcaa cctgttgata gtacgtacta agctctcatg
     7801 tttcacgtac taagctctca tgtttaacgt actaagctct catgtttaac gaactaaacc
     7861 ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg tttcacgtac
     7921 taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat agcctctaag
     7981 gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcctt taaggtttaa
     8041 cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag cctctcaaag
     8101 caattttgag tgacacagga acacttaacg gctgacatgg ggcgcgccca gctgtctagg
     8161 gcggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     8221 cccagtcttt cgactgagcc tttcgtttta tttgatgcct
//
