LOCUS       sequence-417707-       10772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vinculin (chicken) with S1033 unphosphorylatable point mutation 
            (S1033A) tagged with VenusA206K at the C-terminus, in lentiviral 
            expression vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10772)
  AUTHORS   Shoyer TC, Gates EM, Cabe JI, Urs AN, Conway DE, Hoffman BD
  TITLE     Coupling during collective cell migration is controlled by a 
            vinculin mechanochemical switch.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 12;120(50):e2316456120. doi: 
            10.1073/pnas.2316456120. Epub 2023 Dec 6.
  PUBMED    38055737
REFERENCE   2  (bases 1 to 10772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-12"; volume: "120(50):e2316456120. 
            doi"; pages: " 10.1073/pnas.2316456120. Epub 2023 Dec 6"
COMMENT     Sequence Label: pRRL-VinculinVenus-S1033A
FEATURES             Location/Qualifiers
     source          1..10772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    40..156
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(423..467)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    652..885
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1378..1503
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1550..1730
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1731..1957)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1985..2005)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1985..2003)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2024..2040)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2024..2040)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2037..2059)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2048..2064
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2072..2078,2079..2096,2097..2102))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2117..2138
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2255..2272)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2426..3014)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2506..2525)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3185..3976,3977..4045))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3808..3827
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4046..4150)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4176..4631)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4313..4334)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4525..4544
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4758..4780
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4772..4789
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4773..4789
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4799..4817
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4838..4973
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5013..5134
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5024..5043)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5078..5097
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             5206..5439
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5530..6118
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5636..5647
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6045..6065
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(6144..6857,6858..6860,6861..6863))
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6189..6210)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6538..6557
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6797..6818
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(10106..10124)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(10169..10372)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(10198..10218)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        10373..10752
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 acatcgatct tcgaactcga gcccggggga tccatcgata aaattttgaa ttttgtaatt
       61 tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct
      121 gcactgtacc ccccaatccc cccttttctt ttaaaagtta accgataccg tcgagatccg
      181 ttcactaatc gaatggatct gtctctgtct ctctctccac cttcttcttc tattccttcg
      241 ggcctgtcgg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc
      301 tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct
      361 cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc
      421 cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt
      481 tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac
      541 tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc
      601 caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt
      661 tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag
      721 actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc
      781 tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc
      841 atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt
      901 ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt
      961 tttactactt tatatttata taattcactt ctccaattgt ccctcatatc tcctcctcca
     1021 ggtctgaaga tcagcggccg cttgctgtgc ggtggtctta cttttgtttt gctcttcctc
     1081 tatcttgtct aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag
     1141 ggttgctact gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg
     1201 tagctgtccc agtatttgtc tacagccttc tgatgtttct aacaggccag gattaactgc
     1261 gaatcgttct agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc
     1321 ccctggcctt aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac
     1381 gctctcgcac ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac
     1441 cagtcgccgc ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga
     1501 gagctcctct ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt
     1561 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg
     1621 gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct
     1681 agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc gtttatgtat
     1741 cgagctaggc acttaaatac aatatctctg caatgcggca attcagtggt tcgtccaatc
     1801 catgtcagac ccgtctgttg ccttcctaat aaggcacgat cgtaccacct tacttccacc
     1861 aatcggcatg cacggtgctt tttctctcct tgtaaggcat gttgctaact catcgttacc
     1921 atgttgcaag actacaagag tattgcataa gactacatta agcttgcagc tccagctttt
     1981 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     2041 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     2101 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     2161 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     2221 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2281 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2341 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2401 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2461 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2521 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2581 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2641 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccattcagc
     2701 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2761 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2821 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     2881 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2941 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3001 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3061 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3121 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3181 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3241 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     3301 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     3361 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     3421 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     3481 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     3541 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     3601 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     3661 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     3721 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     3781 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     3841 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     3901 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3961 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4021 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4081 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4141 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     4201 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     4261 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     4321 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     4381 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     4441 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     4501 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     4561 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     4621 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     4681 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     4741 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta
     4801 atacgactca ctatagggcg aattgggtac gtccctaggc ctccaaaaaa gcctcctcac
     4861 tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt
     4921 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga
     4981 gttaggggcg ggatagctag agccagacat gataagatac attgatgagt ttggacaaac
     5041 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     5101 atttgtaacc attataagct gcaataaaca agttcctctc actctctgat attcatttct
     5161 ttgcaagtta taaatactga ataataagat gacatgaact actactgcta gagattttcc
     5221 acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac
     5281 acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt
     5341 agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta caagcaaaaa
     5401 gcagatcttg tcttcgttgg gagtgaatta gcccttccag tccccccttt tcttttaaaa
     5461 agtggctaag atctacagct gccttgtaag tcattggtct taaaggtacc gagctcgaat
     5521 taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa
     5581 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga
     5641 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga
     5701 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca
     5761 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag
     5821 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag
     5881 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca
     5941 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac
     6001 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag
     6061 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt
     6121 cgactctaga ctcgagcggc cgcttacttg tacagctcgt ccatgccgag agtgatcccg
     6181 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
     6241 agtttggact ggtagctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
     6301 ggggtgttct gctggtagtg gtcggcgagc tgcacgccgc cgtcctcgat gttgtggcgg
     6361 atcttgaagt tggccttgat gccgttcttc tgcttgtcgg cggtgatata gacgttgtgg
     6421 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
     6481 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
     6541 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
     6601 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcgggcgaa gcactgcagg
     6661 ccgtagccca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
     6721 atcagcttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg
     6781 aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac
     6841 agctcctcgc ccttgctcac catgaattcc tgataccatg gggtctttct gacccagcgc
     6901 agagtgaatc cggcatctgt tcttatctta atggatgctg cttcagcttc tctcacagtt
     6961 tccttcacag cctgcatgag gttctgggcg ttatgaacca acatctcagt tgcctgttct
     7021 gattcttcat cgctgatgtt agtcctgccc agcatggtag ctttcactgt ggaaagaatt
     7081 ttgagctgcg tgctgatggt tgggattcgc tcacagacct gtaagaggtt tgttctaatg
     7141 cgtttatcag tacattgctt ggccacctct ttggccaatc gagtgacttc atccgatgcc
     7201 ttagcaatat cttttgcaca ctggatgagg gcacgcttgt ttccgctgcc tcctcgcacc
     7261 aggcgtgaca tctcagccat gagcagcgcc attcgtttag cagcagcaat gatgtcgtta
     7321 cccttgctag accatttccg agcctcgtca tgcaactgcc tagcagccat catcatgggc
     7381 tgattaatag cttctcctgc tttctgctct gggaactcct catctttttc ttcagggggt
     7441 ggtggtctgg gtgggggaac ctcaccttct ggaagtggtg gttttggagg agcaagctca
     7501 tcagtcagat ggagatgctc taggtcagga ggaggagggg gaaagtctgg ctcctgaggc
     7561 tgaaaggctt ctctgacttt ggccacagct cccagaatcc tgtatccaga atccaagaaa
     7621 ctcttctgca aaccaggatc agagatattt cctgctacag ctttagcatc cattaccatc
     7681 ggtgatatgg ttttgctcag ctcatcagaa gctgctttaa cagcctccct gaatttaggg
     7741 tcttctgaat tttcaacctc ccgttttgcc acaagcagga tgcggttcgc tcgtctagca
     7801 atgctggtgg ctccagctac cagcatctga ggttgcatat tggccattgc aactttacat
     7861 ttatcaagat ccttcttaat agcctcttct gatgcatcca acagagactt ggtatcgatg
     7921 gcctcatcca ccagccctgt catcttttct acattatcaa tccattggtt tttcattgtc
     7981 tcaaaatgct cataagcagc ttgatttcca ggatttctca ggaggattcg agcagccgat
     8041 actacctgtg gtgtaagctc ccttgcagat ttgactgttg cctgaatgcc ttccacagta
     8101 gttttattag cagttccaac tgcagctgct ttttctgctg ttgctcccag tctagcagca
     8161 tggttttcaa aatttgctgc tctttcttca aacacctctt ctctattggg agtatcagaa
     8221 ggagcagtgg ctgctactgc taacaactta ataggggttg tagtgtcact gaaaacatca
     8281 gacacctcct gggtcattgc ttcttgcatc cgtgctttga gatccttcag ggagtcctga
     8341 agctgagcag caattgccct agcctgagga gactctcctt cccctcttgc tgcaaggtca
     8401 gcaagctgag cagccagctg gtctacacgg tcacatttgg caagcaggtc ctgacgataa
     8461 ggtcccatca tgacattggc tagacgacga ccttctgcaa ccaaaccccg aattgctgcc
     8521 tggcctactc cccgatcatc aactgtagga ttatctatcc acctctgggc ttgctcaatc
     8581 ttgccctcca aatggacagc agctttaact ggcctagtat ttgctacagc tctgtttgtt
     8641 ttggactgta agttctgaag tgatgtagct atttgcttgg ccaatgcacg ggcctcagga
     8701 gagtcgcctt tcccatgtcg tcgcagatct gacagcttag ctgtcagagc agagatttcc
     8761 cccaaggagc gaaggatatc atctctttct ttaggctcct cacacaattc tgcaactttc
     8821 cttgcttcag acataattcc tcgaatgtgt tcttctcctt cacttccgcc gttgggatcc
     8881 gcaagccagt tctgagcagc atcaatcttc tttgcaatag cctgcttaga gtttgtcatg
     8941 gcctccaatt tccgggctgc attctccact tttgcagtga gcaaatccag gccttgtgac
     9001 acctgctgtg ccttctgcat tgccatcggt gtagcaccct gtcctctagc tcggagatca
     9061 gcaagttgat cagtcatttg gcccaaagtt ttgcatgttc ccagaatctc cctgcgttct
     9121 ttgcctgcac acaattctcc tgcttttcca gcttcatcaa ggatctgcct tattgcttgc
     9181 tcaccagcat cccctggagg tgcgtttgga tctctcagcc agccttttgc ctggttcatc
     9241 tttgaatcta ttagggctaa agctcttttc atggcttcag tgtccttgct ggcccaggca
     9301 tcttcatccc aggaagtgag ttgtaataca cggattattt catttatctc agcactcatt
     9361 ttctctactg tgaaattgcg atttttcaag gcctcttcta ttccctggct tttagtgttt
     9421 tttgtggtta caaagatctt catagctgaa ataagtacag gcaatagctc cttcacagta
     9481 ttcatggagt tcaccagcat aaccctatgt tcctgatgag ttaattcctg ttgtctctca
     9541 tcgatcattt tcgccatctt tgtcattcct ggccctagat tctttgtata tgtcaccaaa
     9601 tcctccatag tctctactac ttctgccaca gtcagatatt ccaatattcc tttgcagaca
     9661 cggatgattt tacggacctc cgcttcatca aatgtcagaa gtaagtctga tgttccagaa
     9721 aggatgcctc ttgatccatc aattaggtag tcacgagctg gtactgaata aggatctgct
     9781 tgcagcatct gggctgctcg aacgagcttg gtgcaggcat tctctacttt gatgaatgct
     9841 ggtggcatat cccttttcaa gatctggtct tctgttgtct gcacagtttc ttttccaacc
     9901 cgcaccaggt tgctgacagc ggcctgcacg gccgacacgg gggcggtgag gtccgggatg
     9961 gccttgccgt ctacctcccc ctcctcgtgc atgatgacca ggtgggagat ctgctgagcc
    10021 acgggctcca agatgctctc gatggtgcgc gtgtggaaga cgggcatggc aagcttaagt
    10081 ttaaacgcta gccagcttgg gtctccctat agtgagtcgt attaatttcg ataagccagt
    10141 aagcagtggg ttctctagtt agccagagag ctctgcttat atagacctcc caccgtacac
    10201 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
    10261 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
    10321 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
    10381 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
    10441 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
    10501 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
    10561 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
    10621 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
    10681 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca
    10741 ataatcaatg tcaacgcgta tatctggccc gt
//
