LOCUS       sequence-417711-       11488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vinculin conformation sensor (VinCS) with vinculin S1033 
            unphosphorylatable point mutation (S1033A), in lentiviral expression
            vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11488)
  AUTHORS   Shoyer TC, Gates EM, Cabe JI, Urs AN, Conway DE, Hoffman BD
  TITLE     Coupling during collective cell migration is controlled by a 
            vinculin mechanochemical switch.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 12;120(50):e2316456120. doi: 
            10.1073/pnas.2316456120. Epub 2023 Dec 6.
  PUBMED    38055737
REFERENCE   2  (bases 1 to 11488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-12"; volume: "120(50):e2316456120. 
            doi"; pages: " 10.1073/pnas.2316456120. Epub 2023 Dec 6"
COMMENT     Sequence Label: pRRL-VinculinCS-S1033A
FEATURES             Location/Qualifiers
     source          1..11488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    398..514
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(781..825)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1010..1243
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1736..1861
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1908..2088
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2089..2315)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2343..2363)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2343..2361)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2382..2398)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2382..2398)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2395..2417)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2406..2422
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2430..2436,2437..2454,2455..2460))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2475..2496
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2613..2630)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2784..3372)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2864..2883)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3543..4334,4335..4403))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4166..4185
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4404..4508)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4534..4989)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4671..4692)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4883..4902
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5116..5138
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5130..5147
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5131..5147
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5157..5175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      5196..5331
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5371..5492
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5382..5401)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5436..5455
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             5564..5797
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5888..6476
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5994..6005
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6403..6423
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(6495..7208,7209..7211,7212..7214))
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6540..6561)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6889..6908
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7148..7169
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(7779..8486)
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     promoter        complement(11180..11198)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(11243..11446)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(11272..11292)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        join(11447..11488,1..338)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
       61 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
      121 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
      181 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
      241 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt
      301 aattgattac tattaataac tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac
      361 atcgatcttc gaactcgagc ccgggggatc catcgataaa attttgaatt ttgtaatttg
      421 tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc
      481 actgtacccc ccaatccccc cttttctttt aaaagttaac cgataccgtc gagatccgtt
      541 cactaatcga atggatctgt ctctgtctct ctctccacct tcttcttcta ttccttcggg
      601 cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg
      661 cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc
      721 tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca
      781 caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg
      841 cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc
      901 catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca
      961 aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg
     1021 atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac
     1081 tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg
     1141 cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat
     1201 agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt
     1261 ttctctctgc accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt
     1321 tactacttta tatttatata attcacttct ccaattgtcc ctcatatctc ctcctccagg
     1381 tctgaagatc agcggccgct tgctgtgcgg tggtcttact tttgttttgc tcttcctcta
     1441 tcttgtctaa agcttccttg gtgtctttta tctctatcct ttgatgcaca caatagaggg
     1501 ttgctactgt attatataat gatctaagtt cttctgatcc tgtctgaagg gatggttgta
     1561 gctgtcccag tatttgtcta cagccttctg atgtttctaa caggccagga ttaactgcga
     1621 atcgttctag ctccctgctt gcccatacta tatgttttaa tttatatttt ttctttcccc
     1681 ctggccttaa ccgaattttt tcccatcgcg atctaattct cccccgctta atactgacgc
     1741 tctcgcaccc atctctctcc ttctagcctc cgctagtcaa aatttttggc gtactcacca
     1801 gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga
     1861 gctcctctgg tttccctttc gctttcaagt ccctgttcgg gcgccactgc tagagatttt
     1921 ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc
     1981 acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag
     2041 ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccgt ttatgtatcg
     2101 agctaggcac ttaaatacaa tatctctgca atgcggcaat tcagtggttc gtccaatcca
     2161 tgtcagaccc gtctgttgcc ttcctaataa ggcacgatcg taccacctta cttccaccaa
     2221 tcggcatgca cggtgctttt tctctccttg taaggcatgt tgctaactca tcgttaccat
     2281 gttgcaagac tacaagagta ttgcataaga ctacattaag cttgcagctc cagcttttgt
     2341 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     2401 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     2461 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     2521 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     2581 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     2641 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2701 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2761 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2821 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2881 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2941 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3001 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3061 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3121 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3181 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3241 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3301 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3361 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3421 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3481 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3541 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     3601 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3661 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3721 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3781 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3841 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3901 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3961 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     4021 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     4081 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     4141 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     4201 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     4261 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4321 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     4381 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     4441 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     4501 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
     4561 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     4621 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     4681 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     4741 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     4801 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     4861 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     4921 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     4981 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     5041 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     5101 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     5161 acgactcact atagggcgaa ttgggtacgt ccctaggcct ccaaaaaagc ctcctcacta
     5221 cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag
     5281 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     5341 taggggcggg atagctagag ccagacatga taagatacat tgatgagttt ggacaaacca
     5401 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     5461 ttgtaaccat tataagctgc aataaacaag ttcctctcac tctctgatat tcatttcttt
     5521 gcaagttata aatactgaat aataagatga catgaactac tactgctaga gattttccac
     5581 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
     5641 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
     5701 ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca agcaaaaagc
     5761 agatcttgtc ttcgttggga gtgaattagc ccttccagtc cccccttttc ttttaaaaag
     5821 tggctaagat ctacagctgc cttgtaagtc attggtctta aaggtaccga gctcgaatta
     5881 attccaggcg gggaggcggc ccaaagggag atccgactcg tctgagggcg aaggcgaaga
     5941 cgcggaagag gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc tggattgagg
     6001 gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac acaggcgagc
     6061 agccatggaa aggacgtcag cttccccgac aacaccacgg aattgtcagt gcccaacagc
     6121 cgagcccctg tccagcagcg ggcaaggcag gcggcgatga gttccgccgt ggcaataggg
     6181 agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg
     6241 ggggttgcgt cagcaaacac agtgcacacc acgccacgtt gcctgacaac gggccacaac
     6301 tcctcataaa gagacagcaa ccaggattta tacaaggagg agaaaatgaa agccatacgg
     6361 gaagcaatag catgatacaa aggcattaaa gcagcgtatc cacatagcgt aaaaggagca
     6421 acatagttaa gaataccagt caatctttca caaattttgt aatccagagg ttgattgtcg
     6481 actctagaat cgatttactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc
     6541 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagtttggac
     6601 tggtagctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
     6661 tgctggtagt ggtcggcgag ctgcacgccg ccgtcctcga tgttgtggcg gatcttgaag
     6721 ttggccttga tgccgttctt ctgcttgtcg gcggtgatat agacgttgtg gctgttgtag
     6781 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc
     6841 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg
     6901 ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg
     6961 aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga agcactgcag gccgtagccc
     7021 agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatcagcttc
     7081 agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg
     7141 ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg
     7201 cccttgctca ccatgaattc ctgataccat ggggtctttc tgacccagcg cagagtgaat
     7261 ccggcatctg ttcttatctt aatggatgct gcttcagctt ctctcacagt ttccttcaca
     7321 gcctgcatga ggttctgggc gttatgaacc aacatctcag ttgcctgttc tgattcttca
     7381 tcgctgatgt tagtcctgcc cagcatggta gctttcactg tggaaagaat tttgagctgc
     7441 gtgctgatgg ttgggattcg ctcacagacc tgtaagaggt ttgttctaat gcgtttatca
     7501 gtacattgct tggccacctc tttggccaat cgagtgactt catccgatgc cttagcaata
     7561 tcttttgcac actggatgag ggcacgcttg tttccgctgc ctcctcgcac caggcgtgac
     7621 atctcagcca tgagcagcgc cattcgttta gcagcagcaa tgatgtcgtt acccttgcta
     7681 gaccatttcc gagcctcgtc atgcaactgc ctagcagcca tcatcatggg ctgattaata
     7741 gcttctcctg ctttctgctc tgggaactcc gcggccgcct tgtacagctc gtccatgccg
     7801 tcggtggagt tgcgggccac ggcgctctcg taaacggtca ccttgttgta gtccttgtcg
     7861 tggttcagga tctcgatgcg gtggtccaca aagtgatagt cgggcagctt caccgccttc
     7921 ttggccctgt agatggtctt gaagtcaacg cggtggtggc cgccgccctc cagcagcagc
     7981 ttgtgcttga cgtcgccctt cagcacgccg tcgcgcacgt acatcctctc ggtggaggcg
     8041 tcccagccgg tggtcttctt ctgcatcacg gggccgttgg gggggaagtt ctcgcccttg
     8101 aggtgtatct cgtagatgaa ggagtcctcc tccatggaga tgtcggactt caccttcacg
     8161 atgcccttgt cctcgaaggt catggtgcgc tcccaagagt agccctcggg gaaggactgc
     8221 ttgaagtagt tggggatgtc gtcggggtac ttggtgaagg ccctgttgcc gtaggcgaac
     8281 gcggtggtca gaatgtcgta ggagaagggc aggggggctc cctccttcac ctccaggttg
     8341 atggtgttgg tgccgtcgta gggcttgccc tcgccctcgc cctcgatcac gaaggcgtgg
     8401 ccattcacgt tgccctccat cttcagcttg atcttcatgt cgggcttgat tacgcccatt
     8461 gtggtctcct cgcccttgct caccatctcg acctcatctt tttcttcagg gggtggtggt
     8521 ctgggtgggg gaacctcacc ttctggaagt ggtggttttg gaggagcaag ctcatcagtc
     8581 agatggagat gctctaggtc aggaggagga gggggaaagt ctggctcctg aggctgaaag
     8641 gcttctctga ctttggccac agctcccaga atcctgtatc cagaatccaa gaaactcttc
     8701 tgcaaaccag gatcagagat atttcctgct acagctttag catccattac catcggtgat
     8761 atggttttgc tcagctcatc agaagctgct ttaacagcct ccctgaattt agggtcttct
     8821 gaattttcaa cctcccgttt tgccacaagc aggatgcggt tcgctcgtct agcaatgctg
     8881 gtggctccag ctaccagcat ctgaggttgc atattggcca ttgcaacttt acatttatca
     8941 agatccttct taatagcctc ttctgatgca tccaacagag acttggtatc gatggcctca
     9001 tccaccagcc ctgtcatctt ttctacatta tcaatccatt ggtttttcat tgtctcaaaa
     9061 tgctcataag cagcttgatt tccaggattt ctcaggagga ttcgagcagc cgatactacc
     9121 tgtggtgtaa gctcccttgc agatttgact gttgcctgaa tgccttccac agtagtttta
     9181 ttagcagttc caactgcagc tgctttttct gctgttgctc ccagtctagc agcatggttt
     9241 tcaaaatttg ctgctctttc ttcaaacacc tcttctctat tgggagtatc agaaggagca
     9301 gtggctgcta ctgctaacaa cttaataggg gttgtagtgt cactgaaaac atcagacacc
     9361 tcctgggtca ttgcttcttg catccgtgct ttgagatcct tcagggagtc ctgaagctga
     9421 gcagcaattg ccctagcctg aggagactct ccttcccctc ttgctgcaag gtcagcaagc
     9481 tgagcagcca gctggtctac acggtcacat ttggcaagca ggtcctgacg ataaggtccc
     9541 atcatgacat tggctagacg acgaccttct gcaaccaaac cccgaattgc tgcctggcct
     9601 actccccgat catcaactgt aggattatct atccacctct gggcttgctc aatcttgccc
     9661 tccaaatgga cagcagcttt aactggccta gtatttgcta cagctctgtt tgttttggac
     9721 tgtaagttct gaagtgatgt agctatttgc ttggccaatg cacgggcctc aggagagtcg
     9781 cctttcccat gtcgtcgcag atctgacagc ttagctgtca gagcagagat ttcccccaag
     9841 gagcgaagga tatcatctct ttctttaggc tcctcacaca attctgcaac tttccttgct
     9901 tcagacataa ttcctcgaat gtgttcttct ccttcacttc cgccgttggg atccgcaagc
     9961 cagttctgag cagcatcaat cttctttgca atagcctgct tagagtttgt catggcctcc
    10021 aatttccggg ctgcattctc cacttttgca gtgagcaaat ccaggccttg tgacacctgc
    10081 tgtgccttct gcattgccat cggtgtagca ccctgtcctc tagctcggag atcagcaagt
    10141 tgatcagtca tttggcccaa agttttgcat gttcccagaa tctccctgcg ttctttgcct
    10201 gcacacaatt ctcctgcttt tccagcttca tcaaggatct gccttattgc ttgctcacca
    10261 gcatcccctg gaggtgcgtt tggatctctc agccagcctt ttgcctggtt catctttgaa
    10321 tctattaggg ctaaagctct tttcatggct tcagtgtcct tgctggccca ggcatcttca
    10381 tcccaggaag tgagttgtaa tacacggatt atttcattta tctcagcact cattttctct
    10441 actgtgaaat tgcgattttt caaggcctct tctattccct ggcttttagt gttttttgtg
    10501 gttacaaaga tcttcatagc tgaaataagt acaggcaata gctccttcac agtattcatg
    10561 gagttcacca gcataaccct atgttcctga tgagttaatt cctgttgtct ctcatcgatc
    10621 attttcgcca tctttgtcat tcctggccct agattctttg tatatgtcac caaatcctcc
    10681 atagtctcta ctacttctgc cacagtcaga tattccaata ttcctttgca gacacggatg
    10741 attttacgga cctccgcttc atcaaatgtc agaagtaagt ctgatgttcc agaaaggatg
    10801 cctcttgatc catcaattag gtagtcacga gctggtactg aataaggatc tgcttgcagc
    10861 atctgggctg ctcgaacgag cttggtgcag gcattctcta ctttgatgaa tgctggtggc
    10921 atatcccttt tcaagatctg gtcttctgtt gtctgcacag tttcttttcc aacccgcacc
    10981 aggttgctga cagcggcctg cacggccgac acgggggcgg tgaggtccgg gatggccttg
    11041 ccgtctacct ccccctcctc gtgcatgatg accaggtggg agatctgctg agccacgggc
    11101 tccaagatgc tctcgatggt gcgcgtgtgg aagacgggca tggcaagctt aagtttaaac
    11161 gctagccagc ttgggtctcc ctatagtgag tcgtattaat ttcgataagc cagtaagcag
    11221 tgggttctct agttagccag agagctctgc ttatatagac ctcccaccgt acacgcctac
    11281 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
    11341 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
    11401 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
    11461 tgactaatac gtagatgtac tgccaagt
//
