LOCUS       sequence-417729-        8382 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of EGFP-Talin head(1-433)-SpyCatcher003(K31TAG) in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8382)
  AUTHORS   Rahikainen R, Vester SK, Turkki P, Janosko CP, Deiters A, Hytonen 
            VP, Howarth M
  TITLE     Visible Light-Induced Specific Protein Reaction Delineates Early 
            Stages of Cell Adhesion.
  JOURNAL   J Am Chem Soc. 2023 Nov 15;145(45):24459-24465. doi: 
            10.1021/jacs.3c07827. Epub 2023 Oct 31.
  PUBMED    38104267
REFERENCE   2  (bases 1 to 8382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.3c07827"; journalName: "J Am Chem Soc"; date: 
            "2023-11-15- 15"; volume: "145"; issue: "45"; pages: "24459-24465"
COMMENT     Sequence Label: EGFP-Talin head-SpyCatcher003(K31TAG)
FEATURES             Location/Qualifiers
     source          1..8382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(111..325)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(171..190)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        complement(436..676)
                     /label=U6 promoter
                     /note="Truncated RNA polymerase III promoter for human U6 
                     snRNA used in pSIREN vectors such as 
                     pSIREN-RetroQ-ZsGreen1. Expected transcription start site 
                     is 8bp downstream of this feature."
     primer_bind     complement(486..505)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(656..676)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(684..695)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     enhancer        766..1145
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1146..1349
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1300..1320
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1394..1413
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1394..1412
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1454..1456,1457..1459,1460..2170)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1454..1456,1457..1459,1460..2170)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1499..1520)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1760..1779)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2107..2128
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      2219..2228
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3941..3959)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3942..3959)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3979..3996)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3985..4209
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4255..4683
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4342..4361)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4552..4573
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4692..4712)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4697..5026
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4877..5012
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4939..4958
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5093..5887
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5147..5166)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5757..5776
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6061..6182
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6098..6117)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6152..6171
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6231..6247)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6231..6247)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6244..6266)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6255..6271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6279..6285,6286..6303,6304..6309))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6324..6345
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6462..6479)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6633..7221)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6713..6732)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7392..8183,8184..8252))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8015..8034
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8253..8357)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcgacg gatcgggaga tctagatcca aaaaacggaa accccgggaa tctaacccgg
       61 ctgaacggat ttagagtccg ttcgatctac atgatcaggt tcccagagct gtggtctcat
      121 acagaactta taagattccc aaatccaaag acatttcacg tttatggtga tttcccagaa
      181 cacatagcga catgcaaata ttgcagggcg ccactcccct gtccctcaca gccatcttcc
      241 tgccagggcg cacgcgcgct gggtgttccc gcctagtgac actgggcccg cgattccttg
      301 gagcgggttg atgacgtcag cgttcgaatt gactagcaaa aaccgcactt gtcagatcca
      361 aaaaacgggg agtacgggag tttcacccgc ctgcacgaat ttagagtccg tgtgatctat
      421 tcgatcaacc cccccgtcct ttccacaaga tatataaagc caagaaatcg aaatactttc
      481 aagttacggt aagcatatga tagtccattt taaaacataa ttttaaaact gcaaactacc
      541 caagaaatta ttactttcta cgtcacgtat tttgtactaa tatctttgtg tttacagtca
      601 aattaattct aattatctct ctaacagcct tgtatcgtat atgcaaatat gaaggaatca
      661 tgggaaatag gccctcttcc tgcccgacct tcaattgcat gaagaatctg cttagggtta
      721 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
      781 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
      841 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      901 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      961 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     1021 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     1081 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     1141 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     1201 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     1261 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     1321 tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact
     1381 ggcttatcga aattaatacg actcactata gggagaccca agcttggtac cgagctcgga
     1441 tcccgctagc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     1501 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     1561 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     1621 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     1681 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     1741 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     1801 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     1861 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     1921 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     1981 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     2041 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     2101 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     2161 gctgtacaag tccggactca gatctcgagc tcaagctagc ttcgaattcg cagaagctgc
     2221 caccatggtt gcgctttcgc tgaagattag cattgggaat gtggtgaaga cgatgcaatt
     2281 tgagccatct accatggtgt acgacgcctg ccgcatgatt cgtgagcgga tcccagaggc
     2341 cctggctggc cctcccaacg actttgggct ctttctgtca gatgatgacc ccaaaaaagg
     2401 catctggcta gaggctggga aagctttgga ctactacatg ctccgaaatg gggacaccat
     2461 ggagtacaga aaaaagcaga gacccctgaa gatccggatg ttagacggaa cggtgaagac
     2521 tatcatggtg gatgactcca agaccgtcac agacatgctc atgaccattt gtgcccgaat
     2581 tggtatcacc aaccatgatg agtattcact ggttcgagag ctgatggaag aaaagaaaga
     2641 tgaggggaca gggaccctga gaaaagacaa gaccctactg cgagatgaaa agaagatgga
     2701 gaagctaaag cagaagttgc acacagacga tgagttgaac tggctggacc atgggcggac
     2761 actgagggaa cagggagtgg aagagcatga gacgttgctg ctgcggagaa agttcttcta
     2821 ctcagaccag aatgtggatt cccgagaccc tgtacagctg aaccttctct atgtgcaggc
     2881 acgagatgac atcctgaatg gctcccatcc tgtctccttt gacaaggcct gtgaatttgc
     2941 aggcttccag tgccagatcc agtttggacc tcacaatgaa cagaagcaca aggctggctt
     3001 ccttgacctg aaggacttcc tgcccaagga gtatgtgaag cagaagggag agcgtaagat
     3061 ctttcaggca cacaagaatt gtgggcagat gagtgaaatt gaggccaagg tacgctacgt
     3121 gaagctggcc cgttccctca agacttacgg tgtctccttc ttcctagtca aggaaaagat
     3181 gaaggggaag aataaactgg tgcccaggct gttgggcatc actaaggagt gtgtgatgcg
     3241 tgtggatgag aagaccaagg aggtgattca ggaatggagc ctcaccaaca tcaaacgctg
     3301 ggctgcctct cccaagagct ttactctgga ctttggagac tatcaggatg gctactactc
     3361 agtacagacg acagaaggcg agcagatcgc acagctcatt gctggctaca tagatatcat
     3421 ccttaagaag aaaaaaagca aggaccattt tgggctggag ggagatgaag agtctactat
     3481 gttggaggac tcagtgtcac ccaaaaagtc aacagtcctt cagggcgcga tggtgaccac
     3541 actgtccgga ctgtctggag agcagggacc atccggcgac atgaccacag aggaggattc
     3601 tgccacacac atcaagttca gctagaggga cgaggacgga agagagctgg caggagcaac
     3661 catggagctg agggatagct ccggcaagac catcagcaca tggatctccg acggccacgt
     3721 gaaggatttc tacctgtatc ccggcaagta cacctttgtg gagacagcag caccagacgg
     3781 atatgaggtg gcaaccccta tcgagtttac agtgaacgag gacggacagg tgaccgtgga
     3841 tggagaggca acagagggcg atgcacacac ctaatgataa gaattctgca gatatccatc
     3901 acactggcgg ccgctcgagc atgcatctag agggccctat tctatagtgt cacctaaatg
     3961 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     4021 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     4081 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     4141 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     4201 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     4261 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4321 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4381 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     4441 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     4501 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4561 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     4621 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4681 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     4741 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     4801 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4861 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4921 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     4981 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     5041 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     5101 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     5161 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     5221 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     5281 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     5341 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     5401 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     5461 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5521 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     5581 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     5641 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5701 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     5761 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     5821 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     5881 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     5941 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     6001 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     6061 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6121 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6181 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     6241 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     6301 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6361 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6421 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6481 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6541 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     6601 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6661 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6721 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6781 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6841 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6901 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6961 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7021 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7081 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7141 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7201 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7261 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7321 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7381 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7441 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     7501 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     7561 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     7621 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     7681 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     7741 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     7801 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     7861 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     7921 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     7981 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8041 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     8101 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8161 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     8221 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     8281 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     8341 caaatagggg ttccgcgcac atttccccga aaagtgccac ct
//
