LOCUS       sequence-417746-       11053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of SpyTag003(M115G)-Talin rod(434-2541)-mCherry in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11053)
  AUTHORS   Rahikainen R, Vester SK, Turkki P, Janosko CP, Deiters A, Hytonen 
            VP, Howarth M
  TITLE     Visible Light-Induced Specific Protein Reaction Delineates Early 
            Stages of Cell Adhesion.
  JOURNAL   J Am Chem Soc. 2023 Nov 15;145(45):24459-24465. doi: 
            10.1021/jacs.3c07827. Epub 2023 Oct 31.
  PUBMED    38104267
REFERENCE   2  (bases 1 to 11053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.3c07827"; journalName: "J Am Chem Soc"; date: 
            "2023-11-15- 15"; volume: "145"; issue: "45"; pages: "24459-24465"
COMMENT     Sequence Label: SpyTag003(M115G)-Talin rod-mCherry
FEATURES             Location/Qualifiers
     source          1..11053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        83..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      628..637
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7012..7722
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7157..7175)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7425..7444
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    7863..7984
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7900..7919)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7954..7973
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7991..8446)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8128..8149)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8340..8359
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8473..8577
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8579..8936
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8579..8770
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8602..8622)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8787..8922
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8971..9765
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8971..9765
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9025..9044)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9635..9654
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9953..9972)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9997..10044
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10373..10961
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10862..10881
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taaccgtatt accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga
      601 accgtcagat ccgctagcgc taccggtgcc accatgggcc gtggcgtgcc tcatatcgtg
      661 ggagtggacg cctacaagcg ttacaagcag cagtacaacc gagtggggaa agtggagcac
      721 ggctctgtgg ctctgccagc catcatgcgc tctggagcct ctggtcctga gaatttccag
      781 gtggggagca tgccacctgc ccagcagcag atcaccagtg gccagatgca ccgaggacac
      841 atgccacctc tgacttcagc ccagcaggcg ctcactggaa ccattaactc cagcatgcag
      901 gctgtgcagg ctgcccaggc cactctggat gactttgaga ctctaccccc tcttggccag
      961 gatgctgcct ccaaagcttg gcgtaagaac aagatggatg aatcaaagca tgagatccac
     1021 tcccaggtag atgccatcac agctggcact gcctctgtgg taaacctgac agcaggagat
     1081 cctgcagaga cagactatac agcagtgggc tgtgcagtca ccaccatctc ttccaacctg
     1141 acggagatgt cccgtggggt gaagctattg gctgccctgc tggaggatga aggcggcaat
     1201 ggccggcccc ttctgcaagc agcaaagggc cttgcagggg ctgtgtcgga actgcttcgc
     1261 agtgctcagc ccgccagcgc tgagcctcgt cagaacctgc tgcaggcagc cgggaacgtg
     1321 ggccaggcca gtggggagct gttgcagcaa attggggaaa gtgacactga cccccacttc
     1381 caggatgttc taatgcagct agcaaacgca gtggcaagtg ctgcagccgc cctggtcctc
     1441 aaggccaaga gtgtggccca gcgcacagag gattccgggc ttcagaccca agtgattgct
     1501 gcagctacgc aatgtgctct gtccacttcc cagctggtgg cctgtaccaa ggtggtagca
     1561 cctacaatca gctcacctgt gtgccaagaa cagctagtcg aggctgggcg actggtggcc
     1621 aaagctgtgg agggctgtgt gtctgcctcg caagcagcta cagaagatgg acagctgctt
     1681 cgaggggtag gagcagcagc cacggctgtc acccaggccc tcaatgagct gctgcagcac
     1741 gtgaaggccc acgccaccgg agctgggcct gccggtcggt atgaccaagc cactgatacc
     1801 atcctcaccg tcactgaaaa catcttcagc tccatgggtg atgctgggga gatggtgcgc
     1861 caggcccgca tcctggccca agccacctca gacctggtca atgctatcaa ggccgatgct
     1921 gagggggaga gtgatctgga gaactctaga aagctcctga gtgctgccaa gatcctcgct
     1981 gatgccaccg ccaagatggt ggaggcggcc aagggagcag ccgcccaccc tgacagtgag
     2041 gaacagcagc agcgactgcg tgaagcagct gaggggcttc gcatggccac caatgcagct
     2101 gcgcagaacg ccatcaagaa gaagttggtg cagcgcctgg agcatgcagc caagcaagct
     2161 gcagcctctg caacacagac cattgctgca gcccaacatg cagcctctgc ccccaaggcc
     2221 tctgcgggcc cccagcccct actggtgcag agctgtaagg ccgtggcaga gcagattccg
     2281 ttgctggtgc agggtgtccg aggaagtcaa gctcagcctg acagccctag tgctcagctc
     2341 gccctcattg ctgccagcca gagcttcctg cagccaggtg ggaagatggt ggcagcggca
     2401 aaagcctcag tgccaacaat tcaggaccag gcttcagcga tgcagctgag tcagtgcgca
     2461 aagaacctag gcactgccct ggccgagctg cgcactgctg ctcagaaggc tcaggaggca
     2521 tgtggacctt tggagatgga ttctgcactg agtgttgtac aaaatctaga gaaagaccta
     2581 caggaaataa aagcagcagc tcgagatggc aagcttaagc ccttacctgg ggaaacaatg
     2641 gagaagtgta cccaagatct tggcaacagc accaaagcgg tgagctctgc catcgccaag
     2701 ctgctggggg agattgccca gggcaacgag aactatgcag gtattgcagc tcgggatgta
     2761 gctggtggac taaggtcact agcccaggct gcgcggggtg tggctgcgct gacatcagat
     2821 cctgcagtgc aggccattgt gcttgacaca gccagcgacg ttctggacaa ggccagcagc
     2881 ctcattgaag aggcaaaaaa agcatctgga cacccagggg acccagaaag ccagcaaagg
     2941 cttgctcagg tagctaaagc agtgacccaa gccctgaacc gctgtgtcag ctgcctgcct
     3001 ggccaaagag acgtggataa tgccctacga gccgttggag atgccagcaa gcgactcctg
     3061 agtgacttgc ttcctccaag cacggggaca tttcaagaag cacagagccg attgaatgaa
     3121 gctgcagctg ggttaaatca ggccgccaca gaactggtgc aagcctcccg aggaactcct
     3181 caggacctgg ctcgggcctc cggccgattt ggacaggact tcagcacctt cctggaagct
     3241 ggtgtggaga tggccggaca ggccccgagc caggaggacc gagcccaggt ggtgtccaac
     3301 ctgaagggca tatccatgtc ttcaagcaaa cttctccttg ccgctaaggc cttgtccaca
     3361 gaccctgctt ctcccaacct caagagtcag ctggctgcgg ctgcccgggc agtgacggac
     3421 agcatcaacc agctcatcac catgtgcacc cagcaggcac ctggccagaa ggagtgtgac
     3481 aatgcacttc ggcagctaga gacagtccga gaactcctgg agaatccagt ccagcccatc
     3541 aacgacatgt cctacttcgg ttgcttggac agtgtcatgg agaactccaa ggtcctaggt
     3601 gaggccatga ctggcatctc ccaaaatgcc aagaatggaa atctgccgga gtttggagat
     3661 gccattgcca cagcctccaa ggctctctgt ggcttcacgg aggcagccgc acaggcagca
     3721 tatctagttg gtgtctctga ccccaacagc caagctggac agcaaggact ggtggaaccc
     3781 acacagtttg cccgtgcaaa ccaggcaatt cagatggcct gtcagagtct gggggagcct
     3841 ggctgtaccc aggcccaggt gttatctgca gccactattg tagccaaaca cacatctgca
     3901 ttgtgtaaca gctgtcgcct ggcttccgct aggactgcca atcccactgc caagcgccag
     3961 tttgtacagt cagccaagga ggtggccaac agtacagcca atcttgtcaa gaccatcaag
     4021 gcactagatg gggacttcac agaagagaac cgtgcccagt gccgagcagc cacagcccct
     4081 ctgctggaag ctgtggataa cctgagtgcc tttgcctcca accctgagtt ctccagcgtc
     4141 cctgcccaga tcagccctga gggcagagca gccatggagc ccattgtaat ctctgctaag
     4201 acaatgttgg agagtgctgg agggctcatc cagacagctc gggcgttagc agtcaatccc
     4261 cgagaccccc cacgttggtc tgtgctagct ggccactctc ggactgtctc agattccatc
     4321 aagaaactta ttacaagcat gagggacaaa gccccagggc agctggagtg tgagacagcc
     4381 attgcggctc tgaacagctg cttgcgggac ctagatcagg cttcgcttgc cgctgtcagc
     4441 cagcagcttg ctccccgtga aggaatctct caagaggctt tgcacaccca gatgctcact
     4501 gcagtgcaag aaatttctca tctcattgag ccgctggcca gcgctgctcg ggctgaagcc
     4561 tcccagctgg gacacaaggt gtcccaaatg gcccagtact ttgaaccact caccctggct
     4621 gcagtgggtg ctgcgtctaa gaccctgagc cacccgcagc agatggcact tctggaccag
     4681 actaaaacgt tggcagagtc tgccttgcag ttgctatata ctgccaagga ggctggtggt
     4741 aaccccaagc aagcagcaca cacccaggaa gccctggagg aggctgtgca gatgatgaca
     4801 gaggccgtag aagacctgac gacgaccctc aacgaagcag ccagtgctgc aggagtcgtt
     4861 ggcggcatgg tggactctat cactcaggcc atcaaccagc tagatgaagg acctatgggt
     4921 gacccagaag gctcattcgt agattaccag acaaccatgg tgaggacagc caaggccatt
     4981 gctgtcactg ttcaggagat ggtaaccaag tcaaacacca gccctgaaga gctaggccct
     5041 cttgccaacc agctgaccag tgactatggc cgactggcct cacaagccaa gcctgcagct
     5101 gtggctgctg aaaatgaaga gataggcgct cacatcaaac accgagtaca ggagctgggc
     5161 catggctgct ctgctctggt caccaaggca ggtgccttgc agtgtagccc cagtgatgtc
     5221 tacaccaaga aggagctcat agagtgtgcc cgcagagtgt cagagaaggt ctcccatgtt
     5281 ctggctgcac tccaggctgg gaatcgtggt acccaggcct gcattacagc agccagtgct
     5341 gtgtctggta tcattgctga cctcgacacc accatcatgt tcgctactgc tggcacactt
     5401 aaccgtgagg gtgctgaaac ttttgctgac caccgggagg gtatcttaaa gacagcaaag
     5461 gttcttgtgg aggacaccaa ggtcctagtg cagaatgcag ctgggagcca ggagaagttg
     5521 gcacaagccg cccagtcctc cgtggccacc attacccgcc tcgctgatgt ggtcaagctc
     5581 ggtgcagcca gcctaggagc cgaagaccct gaaactcagg tggtgctgat caatgcagta
     5641 aaggacgtag ccaaggccct gggtgacctc atcagcgcta cgaaggctgc agcgggcaaa
     5701 gttggggatg accctgcagt gtggcagctc aagaactctg ccaaggtgat ggtgaccaat
     5761 gtgacatcat tgctcaagac agtgaaggct gtggaagatg aggccaccaa aggcacacgg
     5821 gccctagagg caaccacaga gcacatacgt caggaactgg cggtcttctg ttccccagag
     5881 ccacctgcca agacctctac ccctgaagac ttcatccgaa tgaccaaggg tattactatg
     5941 gcaacagcca aagccgttgc tgctggcaat tcctgtcgac aggaagatgt cattgccaca
     6001 gccaatctga gccgacgggc tattgcggac atgcttcggg cttgcaagga agcagctttc
     6061 cacccagaag tggcgcctga tgtacggctc cgagccctgc actatggccg ggagtgtgcc
     6121 aatggttacc tggaactgct ggaccacgtg ctgctgaccc ttcagaagcc aaacccagac
     6181 ctgaagcagc agctaactgg acactcaaag cgggttgctg gctctgtgac tgagctcatc
     6241 caggctgctg aagccatgaa aggaacagag tgggtggacc cagaggaccc tactgtcatt
     6301 gctgagaatg agctcttggg agccgcagcc gccatcgagg ctgcagccaa gaagctggag
     6361 cagctgaagc ccagggccaa acccaaggag gcggatgagt ccttgaactt tgaggaacaa
     6421 atcctagaag ctgccaagtc catcgctgca gccaccagtg cactggtaaa ggctgcgtca
     6481 gcagcccaga gggagctggt ggctcaagga aaggtgggcg ccattccagc caatgcactg
     6541 gatgatgggc agtggtcgca gggcctcatt tccgctgccc gtatggtggc tgcggccacc
     6601 aacaatctgt gtgaagcagc taacgcagct gtccagggcc atgctagcca ggagaaactc
     6661 atttcctcag ccaagcaagt agctgcctcc acagctcagc tcctggtagc ttgcaaggtc
     6721 aaagcagatc aggactctga ggcaatgaaa cggctccagg ctgctggcaa tgcagtgaag
     6781 agggcctcag ataacctggt aaaggcggcc cagaaagctg cagccttcga agaccaggag
     6841 aatgagacgg tggtggtgaa ggagaaaatg gttgggggca ttgcccagat tatcgcagca
     6901 caggaagaga tgcttcggaa ggaacgagag ctggaagagg ctcggaaaaa gctcgcccag
     6961 atccggcagc agcagtacaa gttcttgcct tcagagcttc gagacgagca catggtgagc
     7021 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     7081 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     7141 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     7201 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     7261 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     7321 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     7381 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     7441 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     7501 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     7561 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     7621 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     7681 ggccgccact ccaccggcgg catggacgag ctgtacaagt aaccgcgggc ccgggatcca
     7741 ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     7801 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     7861 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7921 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7981 cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     8041 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     8101 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     8161 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     8221 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     8281 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     8341 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     8401 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt
     8461 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     8521 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct
     8581 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     8641 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     8701 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     8761 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     8821 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     8881 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc
     8941 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     9001 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     9061 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     9121 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     9181 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     9241 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     9301 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     9361 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     9421 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     9481 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     9541 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     9601 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     9661 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     9721 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     9781 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     9841 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     9901 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac
     9961 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa
    10021 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
    10081 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
    10141 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
    10201 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat
    10261 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    10321 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    10381 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    10441 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    10501 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    10561 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    10621 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    10681 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    10741 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    10801 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    10861 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    10921 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    10981 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    11041 ctgattctgt gga
//
