LOCUS       sequence-417749-        2846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CDS for mCherry in domestication vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Huang L, Rojas-Pierce M
  TITLE     Rapid depletion of target proteins in plants by an inducible protein
            degradation system.
  JOURNAL   Plant Cell. 2024 Mar 6:koae072. doi: 10.1093/plcell/koae072.
  PUBMED    38446628
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2024 Mar 6:koae072. doi: 10.1093/plcell/koae072."
COMMENT     Sequence Label: pUPD2-N-L-mCherry2 (B5)
FEATURES             Location/Qualifiers
     source          1..2846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      803..860
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(884..901)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1055..1643)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1135..1154)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1826..1920)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1941..2600)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2515..2534
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2762..2780
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2784..2827)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    join(2830..2846,1..5)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tagagcgatg agtggtctcg ttcggaggag gtggctcatc tggcggaggt atggtgagta
       61 agggtgaaga agataatatg gcaataatta aagaatttat gcgttttaaa gtccatatgg
      121 aaggatcagt caatggtcat gaattcgaaa ttgaggggga aggtgaagga aggccgtatg
      181 aggggactca gaccgctaaa ctcaaggtga ccaagggtgg gcctttgccg tttgcatggg
      241 atatcctctc tccacaattt atgtatggat caaaggctta cgtcaagcac ccggctgaca
      301 tacctgacta tcttaaactc tctttcccgg aaggttttaa gtgggaaaga gtgatgaatt
      361 tcgaagatgg tggggttgtg accgttacac aagattcaag tctgcaggat ggagagttta
      421 tttacaaggt taaactcaga ggtaccaatt tcccgtctga cgggcctgtg atgcaaaaaa
      481 agactatggg gtgggaggcc tcttcagaaa gaatgtatcc tgaggatgga gcactcaagg
      541 gtgaaattaa acaaagattg aagcttaaag atggaggtca ttatgacgcc gaagtgaaaa
      601 ctacatataa ggcaaagaag ccagtccaat tgccgggagc atataatgtg aatattaaac
      661 tcgatatcac ctctcacaat gaagattata ctatcgtcga acagtatgaa agggccgaag
      721 gtcgtcattc aaccggaggt atggatgagc tctataagtg agctttgaga ccactcatcg
      781 cgactagtag cggccgctgc agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt
      841 tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc
      901 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      961 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1021 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg
     1081 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1141 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1201 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1261 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1321 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1381 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1441 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     1501 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1561 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1621 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1681 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1741 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1801 cttggtctga cagctcgagg cttggattct caccaataaa aaacgcccgg cggcaaccga
     1861 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     1921 caagcgagct cgatatcaaa ttacgccccg ccctgccact cgtcgcagta ctgttgtaat
     1981 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc
     2041 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg
     2101 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
     2161 gctgatacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg
     2221 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca
     2281 ctccagagtg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca
     2341 ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc
     2401 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg
     2461 gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact
     2521 gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat
     2581 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa
     2641 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg
     2701 atcaactcga gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     2761 aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga
     2821 tgatttctgg aattcgcggc cgcttc
//
