LOCUS       sequence-417751-       11053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of SpyTag003(V114T, V116T)-Talin rod(434-2541)-mCherry in
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11053)
  AUTHORS   Rahikainen R, Vester SK, Turkki P, Janosko CP, Deiters A, Hytonen 
            VP, Howarth M
  TITLE     Visible Light-Induced Specific Protein Reaction Delineates Early 
            Stages of Cell Adhesion.
  JOURNAL   J Am Chem Soc. 2023 Nov 15;145(45):24459-24465. doi: 
            10.1021/jacs.3c07827. Epub 2023 Oct 31.
  PUBMED    38104267
REFERENCE   2  (bases 1 to 11053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.3c07827"; journalName: "J Am Chem Soc"; date: 
            "2023-11-15- 15"; volume: "145"; issue: "45"; pages: "24459-24465"
COMMENT     Sequence Label: SpyTag003(V114T, V116T)-Talin rod-mCherry
FEATURES             Location/Qualifiers
     source          1..11053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        93..396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        397..600
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     551..571
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      638..647
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7022..7732
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7167..7185)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7435..7454
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    7873..7994
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7910..7929)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7964..7983
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8001..8456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8138..8159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8350..8369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8483..8587
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8589..8946
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8589..8780
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8612..8632)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8797..8932
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8981..9775
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8981..9775
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9035..9054)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9645..9664
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9963..9982)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10007..10054
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10383..10971
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10872..10891
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attctgtgga taaccgtatt accgccatgc attagttatt aatagtaatc aattacgggg
       61 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      121 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      181 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      241 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      301 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      361 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      421 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      481 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      541 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      601 ggtttagtga accgtcagat ccgctagcgc taccggtgcc accatgggcc gtggcgtgcc
      661 tcatatcacc atgacagacg cctacaagcg ttacaagcag cagtacaacc gagtggggaa
      721 agtggagcac ggctctgtgg ctctgccagc catcatgcgc tctggagcct ctggtcctga
      781 gaatttccag gtggggagca tgccacctgc ccagcagcag atcaccagtg gccagatgca
      841 ccgaggacac atgccacctc tgacttcagc ccagcaggcg ctcactggaa ccattaactc
      901 cagcatgcag gctgtgcagg ctgcccaggc cactctggat gactttgaga ctctaccccc
      961 tcttggccag gatgctgcct ccaaagcttg gcgtaagaac aagatggatg aatcaaagca
     1021 tgagatccac tcccaggtag atgccatcac agctggcact gcctctgtgg taaacctgac
     1081 agcaggagat cctgcagaga cagactatac agcagtgggc tgtgcagtca ccaccatctc
     1141 ttccaacctg acggagatgt cccgtggggt gaagctattg gctgccctgc tggaggatga
     1201 aggcggcaat ggccggcccc ttctgcaagc agcaaagggc cttgcagggg ctgtgtcgga
     1261 actgcttcgc agtgctcagc ccgccagcgc tgagcctcgt cagaacctgc tgcaggcagc
     1321 cgggaacgtg ggccaggcca gtggggagct gttgcagcaa attggggaaa gtgacactga
     1381 cccccacttc caggatgttc taatgcagct agcaaacgca gtggcaagtg ctgcagccgc
     1441 cctggtcctc aaggccaaga gtgtggccca gcgcacagag gattccgggc ttcagaccca
     1501 agtgattgct gcagctacgc aatgtgctct gtccacttcc cagctggtgg cctgtaccaa
     1561 ggtggtagca cctacaatca gctcacctgt gtgccaagaa cagctagtcg aggctgggcg
     1621 actggtggcc aaagctgtgg agggctgtgt gtctgcctcg caagcagcta cagaagatgg
     1681 acagctgctt cgaggggtag gagcagcagc cacggctgtc acccaggccc tcaatgagct
     1741 gctgcagcac gtgaaggccc acgccaccgg agctgggcct gccggtcggt atgaccaagc
     1801 cactgatacc atcctcaccg tcactgaaaa catcttcagc tccatgggtg atgctgggga
     1861 gatggtgcgc caggcccgca tcctggccca agccacctca gacctggtca atgctatcaa
     1921 ggccgatgct gagggggaga gtgatctgga gaactctaga aagctcctga gtgctgccaa
     1981 gatcctcgct gatgccaccg ccaagatggt ggaggcggcc aagggagcag ccgcccaccc
     2041 tgacagtgag gaacagcagc agcgactgcg tgaagcagct gaggggcttc gcatggccac
     2101 caatgcagct gcgcagaacg ccatcaagaa gaagttggtg cagcgcctgg agcatgcagc
     2161 caagcaagct gcagcctctg caacacagac cattgctgca gcccaacatg cagcctctgc
     2221 ccccaaggcc tctgcgggcc cccagcccct actggtgcag agctgtaagg ccgtggcaga
     2281 gcagattccg ttgctggtgc agggtgtccg aggaagtcaa gctcagcctg acagccctag
     2341 tgctcagctc gccctcattg ctgccagcca gagcttcctg cagccaggtg ggaagatggt
     2401 ggcagcggca aaagcctcag tgccaacaat tcaggaccag gcttcagcga tgcagctgag
     2461 tcagtgcgca aagaacctag gcactgccct ggccgagctg cgcactgctg ctcagaaggc
     2521 tcaggaggca tgtggacctt tggagatgga ttctgcactg agtgttgtac aaaatctaga
     2581 gaaagaccta caggaaataa aagcagcagc tcgagatggc aagcttaagc ccttacctgg
     2641 ggaaacaatg gagaagtgta cccaagatct tggcaacagc accaaagcgg tgagctctgc
     2701 catcgccaag ctgctggggg agattgccca gggcaacgag aactatgcag gtattgcagc
     2761 tcgggatgta gctggtggac taaggtcact agcccaggct gcgcggggtg tggctgcgct
     2821 gacatcagat cctgcagtgc aggccattgt gcttgacaca gccagcgacg ttctggacaa
     2881 ggccagcagc ctcattgaag aggcaaaaaa agcatctgga cacccagggg acccagaaag
     2941 ccagcaaagg cttgctcagg tagctaaagc agtgacccaa gccctgaacc gctgtgtcag
     3001 ctgcctgcct ggccaaagag acgtggataa tgccctacga gccgttggag atgccagcaa
     3061 gcgactcctg agtgacttgc ttcctccaag cacggggaca tttcaagaag cacagagccg
     3121 attgaatgaa gctgcagctg ggttaaatca ggccgccaca gaactggtgc aagcctcccg
     3181 aggaactcct caggacctgg ctcgggcctc cggccgattt ggacaggact tcagcacctt
     3241 cctggaagct ggtgtggaga tggccggaca ggccccgagc caggaggacc gagcccaggt
     3301 ggtgtccaac ctgaagggca tatccatgtc ttcaagcaaa cttctccttg ccgctaaggc
     3361 cttgtccaca gaccctgctt ctcccaacct caagagtcag ctggctgcgg ctgcccgggc
     3421 agtgacggac agcatcaacc agctcatcac catgtgcacc cagcaggcac ctggccagaa
     3481 ggagtgtgac aatgcacttc ggcagctaga gacagtccga gaactcctgg agaatccagt
     3541 ccagcccatc aacgacatgt cctacttcgg ttgcttggac agtgtcatgg agaactccaa
     3601 ggtcctaggt gaggccatga ctggcatctc ccaaaatgcc aagaatggaa atctgccgga
     3661 gtttggagat gccattgcca cagcctccaa ggctctctgt ggcttcacgg aggcagccgc
     3721 acaggcagca tatctagttg gtgtctctga ccccaacagc caagctggac agcaaggact
     3781 ggtggaaccc acacagtttg cccgtgcaaa ccaggcaatt cagatggcct gtcagagtct
     3841 gggggagcct ggctgtaccc aggcccaggt gttatctgca gccactattg tagccaaaca
     3901 cacatctgca ttgtgtaaca gctgtcgcct ggcttccgct aggactgcca atcccactgc
     3961 caagcgccag tttgtacagt cagccaagga ggtggccaac agtacagcca atcttgtcaa
     4021 gaccatcaag gcactagatg gggacttcac agaagagaac cgtgcccagt gccgagcagc
     4081 cacagcccct ctgctggaag ctgtggataa cctgagtgcc tttgcctcca accctgagtt
     4141 ctccagcgtc cctgcccaga tcagccctga gggcagagca gccatggagc ccattgtaat
     4201 ctctgctaag acaatgttgg agagtgctgg agggctcatc cagacagctc gggcgttagc
     4261 agtcaatccc cgagaccccc cacgttggtc tgtgctagct ggccactctc ggactgtctc
     4321 agattccatc aagaaactta ttacaagcat gagggacaaa gccccagggc agctggagtg
     4381 tgagacagcc attgcggctc tgaacagctg cttgcgggac ctagatcagg cttcgcttgc
     4441 cgctgtcagc cagcagcttg ctccccgtga aggaatctct caagaggctt tgcacaccca
     4501 gatgctcact gcagtgcaag aaatttctca tctcattgag ccgctggcca gcgctgctcg
     4561 ggctgaagcc tcccagctgg gacacaaggt gtcccaaatg gcccagtact ttgaaccact
     4621 caccctggct gcagtgggtg ctgcgtctaa gaccctgagc cacccgcagc agatggcact
     4681 tctggaccag actaaaacgt tggcagagtc tgccttgcag ttgctatata ctgccaagga
     4741 ggctggtggt aaccccaagc aagcagcaca cacccaggaa gccctggagg aggctgtgca
     4801 gatgatgaca gaggccgtag aagacctgac gacgaccctc aacgaagcag ccagtgctgc
     4861 aggagtcgtt ggcggcatgg tggactctat cactcaggcc atcaaccagc tagatgaagg
     4921 acctatgggt gacccagaag gctcattcgt agattaccag acaaccatgg tgaggacagc
     4981 caaggccatt gctgtcactg ttcaggagat ggtaaccaag tcaaacacca gccctgaaga
     5041 gctaggccct cttgccaacc agctgaccag tgactatggc cgactggcct cacaagccaa
     5101 gcctgcagct gtggctgctg aaaatgaaga gataggcgct cacatcaaac accgagtaca
     5161 ggagctgggc catggctgct ctgctctggt caccaaggca ggtgccttgc agtgtagccc
     5221 cagtgatgtc tacaccaaga aggagctcat agagtgtgcc cgcagagtgt cagagaaggt
     5281 ctcccatgtt ctggctgcac tccaggctgg gaatcgtggt acccaggcct gcattacagc
     5341 agccagtgct gtgtctggta tcattgctga cctcgacacc accatcatgt tcgctactgc
     5401 tggcacactt aaccgtgagg gtgctgaaac ttttgctgac caccgggagg gtatcttaaa
     5461 gacagcaaag gttcttgtgg aggacaccaa ggtcctagtg cagaatgcag ctgggagcca
     5521 ggagaagttg gcacaagccg cccagtcctc cgtggccacc attacccgcc tcgctgatgt
     5581 ggtcaagctc ggtgcagcca gcctaggagc cgaagaccct gaaactcagg tggtgctgat
     5641 caatgcagta aaggacgtag ccaaggccct gggtgacctc atcagcgcta cgaaggctgc
     5701 agcgggcaaa gttggggatg accctgcagt gtggcagctc aagaactctg ccaaggtgat
     5761 ggtgaccaat gtgacatcat tgctcaagac agtgaaggct gtggaagatg aggccaccaa
     5821 aggcacacgg gccctagagg caaccacaga gcacatacgt caggaactgg cggtcttctg
     5881 ttccccagag ccacctgcca agacctctac ccctgaagac ttcatccgaa tgaccaaggg
     5941 tattactatg gcaacagcca aagccgttgc tgctggcaat tcctgtcgac aggaagatgt
     6001 cattgccaca gccaatctga gccgacgggc tattgcggac atgcttcggg cttgcaagga
     6061 agcagctttc cacccagaag tggcgcctga tgtacggctc cgagccctgc actatggccg
     6121 ggagtgtgcc aatggttacc tggaactgct ggaccacgtg ctgctgaccc ttcagaagcc
     6181 aaacccagac ctgaagcagc agctaactgg acactcaaag cgggttgctg gctctgtgac
     6241 tgagctcatc caggctgctg aagccatgaa aggaacagag tgggtggacc cagaggaccc
     6301 tactgtcatt gctgagaatg agctcttggg agccgcagcc gccatcgagg ctgcagccaa
     6361 gaagctggag cagctgaagc ccagggccaa acccaaggag gcggatgagt ccttgaactt
     6421 tgaggaacaa atcctagaag ctgccaagtc catcgctgca gccaccagtg cactggtaaa
     6481 ggctgcgtca gcagcccaga gggagctggt ggctcaagga aaggtgggcg ccattccagc
     6541 caatgcactg gatgatgggc agtggtcgca gggcctcatt tccgctgccc gtatggtggc
     6601 tgcggccacc aacaatctgt gtgaagcagc taacgcagct gtccagggcc atgctagcca
     6661 ggagaaactc atttcctcag ccaagcaagt agctgcctcc acagctcagc tcctggtagc
     6721 ttgcaaggtc aaagcagatc aggactctga ggcaatgaaa cggctccagg ctgctggcaa
     6781 tgcagtgaag agggcctcag ataacctggt aaaggcggcc cagaaagctg cagccttcga
     6841 agaccaggag aatgagacgg tggtggtgaa ggagaaaatg gttgggggca ttgcccagat
     6901 tatcgcagca caggaagaga tgcttcggaa ggaacgagag ctggaagagg ctcggaaaaa
     6961 gctcgcccag atccggcagc agcagtacaa gttcttgcct tcagagcttc gagacgagca
     7021 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     7081 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     7141 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     7201 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     7261 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     7321 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     7381 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     7441 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     7501 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     7561 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     7621 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     7681 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt aaccgcgggc
     7741 ccgggatcca ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt
     7801 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     7861 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7921 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7981 atcaatgtat cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     8041 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     8101 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     8161 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     8221 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     8281 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     8341 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     8401 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
     8461 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8521 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     8581 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
     8641 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     8701 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     8761 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     8821 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     8881 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     8941 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     9001 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     9061 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     9121 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     9181 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     9241 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     9301 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     9361 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     9421 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     9481 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     9541 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     9601 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     9661 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     9721 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     9781 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     9841 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     9901 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta
     9961 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
    10021 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
    10081 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
    10141 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
    10201 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt
    10261 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    10321 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    10381 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    10441 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    10501 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    10561 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    10621 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    10681 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    10741 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    10801 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    10861 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    10921 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    10981 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    11041 gcgttatccc ctg
//
