LOCUS       sequence-417759-       11053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of SpyTag003(M115A)-Talin rod(434-2541)-mCherry in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11053)
  AUTHORS   Rahikainen R, Vester SK, Turkki P, Janosko CP, Deiters A, Hytonen 
            VP, Howarth M
  TITLE     Visible Light-Induced Specific Protein Reaction Delineates Early 
            Stages of Cell Adhesion.
  JOURNAL   J Am Chem Soc. 2023 Nov 15;145(45):24459-24465. doi: 
            10.1021/jacs.3c07827. Epub 2023 Oct 31.
  PUBMED    38104267
REFERENCE   2  (bases 1 to 11053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.3c07827"; journalName: "J Am Chem Soc"; date: 
            "2023-11-15- 15"; volume: "145"; issue: "45"; pages: "24459-24465"
COMMENT     Sequence Label: SpyTag003(M115A)-Talin rod-mCherry
FEATURES             Location/Qualifiers
     source          1..11053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        35..338
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        339..542
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     493..513
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      580..589
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6964..7674
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7109..7127)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7377..7396
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    7815..7936
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7852..7871)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7906..7925
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7943..8398)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8080..8101)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8292..8311
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8425..8529
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8531..8888
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8531..8722
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8554..8574)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8739..8874
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8923..9717
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8923..9717
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8977..8996)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9587..9606
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9905..9924)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9949..9996
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10325..10913
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10814..10833
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
       61 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      121 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      181 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      241 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      301 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      361 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      421 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      481 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      541 ctggtttagt gaaccgtcag atccgctagc gctaccggtg ccaccatggg ccgtggcgtg
      601 cctcatatcg tggctgtgga cgcctacaag cgttacaagc agcagtacaa ccgagtgggg
      661 aaagtggagc acggctctgt ggctctgcca gccatcatgc gctctggagc ctctggtcct
      721 gagaatttcc aggtggggag catgccacct gcccagcagc agatcaccag tggccagatg
      781 caccgaggac acatgccacc tctgacttca gcccagcagg cgctcactgg aaccattaac
      841 tccagcatgc aggctgtgca ggctgcccag gccactctgg atgactttga gactctaccc
      901 cctcttggcc aggatgctgc ctccaaagct tggcgtaaga acaagatgga tgaatcaaag
      961 catgagatcc actcccaggt agatgccatc acagctggca ctgcctctgt ggtaaacctg
     1021 acagcaggag atcctgcaga gacagactat acagcagtgg gctgtgcagt caccaccatc
     1081 tcttccaacc tgacggagat gtcccgtggg gtgaagctat tggctgccct gctggaggat
     1141 gaaggcggca atggccggcc ccttctgcaa gcagcaaagg gccttgcagg ggctgtgtcg
     1201 gaactgcttc gcagtgctca gcccgccagc gctgagcctc gtcagaacct gctgcaggca
     1261 gccgggaacg tgggccaggc cagtggggag ctgttgcagc aaattgggga aagtgacact
     1321 gacccccact tccaggatgt tctaatgcag ctagcaaacg cagtggcaag tgctgcagcc
     1381 gccctggtcc tcaaggccaa gagtgtggcc cagcgcacag aggattccgg gcttcagacc
     1441 caagtgattg ctgcagctac gcaatgtgct ctgtccactt cccagctggt ggcctgtacc
     1501 aaggtggtag cacctacaat cagctcacct gtgtgccaag aacagctagt cgaggctggg
     1561 cgactggtgg ccaaagctgt ggagggctgt gtgtctgcct cgcaagcagc tacagaagat
     1621 ggacagctgc ttcgaggggt aggagcagca gccacggctg tcacccaggc cctcaatgag
     1681 ctgctgcagc acgtgaaggc ccacgccacc ggagctgggc ctgccggtcg gtatgaccaa
     1741 gccactgata ccatcctcac cgtcactgaa aacatcttca gctccatggg tgatgctggg
     1801 gagatggtgc gccaggcccg catcctggcc caagccacct cagacctggt caatgctatc
     1861 aaggccgatg ctgaggggga gagtgatctg gagaactcta gaaagctcct gagtgctgcc
     1921 aagatcctcg ctgatgccac cgccaagatg gtggaggcgg ccaagggagc agccgcccac
     1981 cctgacagtg aggaacagca gcagcgactg cgtgaagcag ctgaggggct tcgcatggcc
     2041 accaatgcag ctgcgcagaa cgccatcaag aagaagttgg tgcagcgcct ggagcatgca
     2101 gccaagcaag ctgcagcctc tgcaacacag accattgctg cagcccaaca tgcagcctct
     2161 gcccccaagg cctctgcggg cccccagccc ctactggtgc agagctgtaa ggccgtggca
     2221 gagcagattc cgttgctggt gcagggtgtc cgaggaagtc aagctcagcc tgacagccct
     2281 agtgctcagc tcgccctcat tgctgccagc cagagcttcc tgcagccagg tgggaagatg
     2341 gtggcagcgg caaaagcctc agtgccaaca attcaggacc aggcttcagc gatgcagctg
     2401 agtcagtgcg caaagaacct aggcactgcc ctggccgagc tgcgcactgc tgctcagaag
     2461 gctcaggagg catgtggacc tttggagatg gattctgcac tgagtgttgt acaaaatcta
     2521 gagaaagacc tacaggaaat aaaagcagca gctcgagatg gcaagcttaa gcccttacct
     2581 ggggaaacaa tggagaagtg tacccaagat cttggcaaca gcaccaaagc ggtgagctct
     2641 gccatcgcca agctgctggg ggagattgcc cagggcaacg agaactatgc aggtattgca
     2701 gctcgggatg tagctggtgg actaaggtca ctagcccagg ctgcgcgggg tgtggctgcg
     2761 ctgacatcag atcctgcagt gcaggccatt gtgcttgaca cagccagcga cgttctggac
     2821 aaggccagca gcctcattga agaggcaaaa aaagcatctg gacacccagg ggacccagaa
     2881 agccagcaaa ggcttgctca ggtagctaaa gcagtgaccc aagccctgaa ccgctgtgtc
     2941 agctgcctgc ctggccaaag agacgtggat aatgccctac gagccgttgg agatgccagc
     3001 aagcgactcc tgagtgactt gcttcctcca agcacgggga catttcaaga agcacagagc
     3061 cgattgaatg aagctgcagc tgggttaaat caggccgcca cagaactggt gcaagcctcc
     3121 cgaggaactc ctcaggacct ggctcgggcc tccggccgat ttggacagga cttcagcacc
     3181 ttcctggaag ctggtgtgga gatggccgga caggccccga gccaggagga ccgagcccag
     3241 gtggtgtcca acctgaaggg catatccatg tcttcaagca aacttctcct tgccgctaag
     3301 gccttgtcca cagaccctgc ttctcccaac ctcaagagtc agctggctgc ggctgcccgg
     3361 gcagtgacgg acagcatcaa ccagctcatc accatgtgca cccagcaggc acctggccag
     3421 aaggagtgtg acaatgcact tcggcagcta gagacagtcc gagaactcct ggagaatcca
     3481 gtccagccca tcaacgacat gtcctacttc ggttgcttgg acagtgtcat ggagaactcc
     3541 aaggtcctag gtgaggccat gactggcatc tcccaaaatg ccaagaatgg aaatctgccg
     3601 gagtttggag atgccattgc cacagcctcc aaggctctct gtggcttcac ggaggcagcc
     3661 gcacaggcag catatctagt tggtgtctct gaccccaaca gccaagctgg acagcaagga
     3721 ctggtggaac ccacacagtt tgcccgtgca aaccaggcaa ttcagatggc ctgtcagagt
     3781 ctgggggagc ctggctgtac ccaggcccag gtgttatctg cagccactat tgtagccaaa
     3841 cacacatctg cattgtgtaa cagctgtcgc ctggcttccg ctaggactgc caatcccact
     3901 gccaagcgcc agtttgtaca gtcagccaag gaggtggcca acagtacagc caatcttgtc
     3961 aagaccatca aggcactaga tggggacttc acagaagaga accgtgccca gtgccgagca
     4021 gccacagccc ctctgctgga agctgtggat aacctgagtg cctttgcctc caaccctgag
     4081 ttctccagcg tccctgccca gatcagccct gagggcagag cagccatgga gcccattgta
     4141 atctctgcta agacaatgtt ggagagtgct ggagggctca tccagacagc tcgggcgtta
     4201 gcagtcaatc cccgagaccc cccacgttgg tctgtgctag ctggccactc tcggactgtc
     4261 tcagattcca tcaagaaact tattacaagc atgagggaca aagccccagg gcagctggag
     4321 tgtgagacag ccattgcggc tctgaacagc tgcttgcggg acctagatca ggcttcgctt
     4381 gccgctgtca gccagcagct tgctccccgt gaaggaatct ctcaagaggc tttgcacacc
     4441 cagatgctca ctgcagtgca agaaatttct catctcattg agccgctggc cagcgctgct
     4501 cgggctgaag cctcccagct gggacacaag gtgtcccaaa tggcccagta ctttgaacca
     4561 ctcaccctgg ctgcagtggg tgctgcgtct aagaccctga gccacccgca gcagatggca
     4621 cttctggacc agactaaaac gttggcagag tctgccttgc agttgctata tactgccaag
     4681 gaggctggtg gtaaccccaa gcaagcagca cacacccagg aagccctgga ggaggctgtg
     4741 cagatgatga cagaggccgt agaagacctg acgacgaccc tcaacgaagc agccagtgct
     4801 gcaggagtcg ttggcggcat ggtggactct atcactcagg ccatcaacca gctagatgaa
     4861 ggacctatgg gtgacccaga aggctcattc gtagattacc agacaaccat ggtgaggaca
     4921 gccaaggcca ttgctgtcac tgttcaggag atggtaacca agtcaaacac cagccctgaa
     4981 gagctaggcc ctcttgccaa ccagctgacc agtgactatg gccgactggc ctcacaagcc
     5041 aagcctgcag ctgtggctgc tgaaaatgaa gagataggcg ctcacatcaa acaccgagta
     5101 caggagctgg gccatggctg ctctgctctg gtcaccaagg caggtgcctt gcagtgtagc
     5161 cccagtgatg tctacaccaa gaaggagctc atagagtgtg cccgcagagt gtcagagaag
     5221 gtctcccatg ttctggctgc actccaggct gggaatcgtg gtacccaggc ctgcattaca
     5281 gcagccagtg ctgtgtctgg tatcattgct gacctcgaca ccaccatcat gttcgctact
     5341 gctggcacac ttaaccgtga gggtgctgaa acttttgctg accaccggga gggtatctta
     5401 aagacagcaa aggttcttgt ggaggacacc aaggtcctag tgcagaatgc agctgggagc
     5461 caggagaagt tggcacaagc cgcccagtcc tccgtggcca ccattacccg cctcgctgat
     5521 gtggtcaagc tcggtgcagc cagcctagga gccgaagacc ctgaaactca ggtggtgctg
     5581 atcaatgcag taaaggacgt agccaaggcc ctgggtgacc tcatcagcgc tacgaaggct
     5641 gcagcgggca aagttgggga tgaccctgca gtgtggcagc tcaagaactc tgccaaggtg
     5701 atggtgacca atgtgacatc attgctcaag acagtgaagg ctgtggaaga tgaggccacc
     5761 aaaggcacac gggccctaga ggcaaccaca gagcacatac gtcaggaact ggcggtcttc
     5821 tgttccccag agccacctgc caagacctct acccctgaag acttcatccg aatgaccaag
     5881 ggtattacta tggcaacagc caaagccgtt gctgctggca attcctgtcg acaggaagat
     5941 gtcattgcca cagccaatct gagccgacgg gctattgcgg acatgcttcg ggcttgcaag
     6001 gaagcagctt tccacccaga agtggcgcct gatgtacggc tccgagccct gcactatggc
     6061 cgggagtgtg ccaatggtta cctggaactg ctggaccacg tgctgctgac ccttcagaag
     6121 ccaaacccag acctgaagca gcagctaact ggacactcaa agcgggttgc tggctctgtg
     6181 actgagctca tccaggctgc tgaagccatg aaaggaacag agtgggtgga cccagaggac
     6241 cctactgtca ttgctgagaa tgagctcttg ggagccgcag ccgccatcga ggctgcagcc
     6301 aagaagctgg agcagctgaa gcccagggcc aaacccaagg aggcggatga gtccttgaac
     6361 tttgaggaac aaatcctaga agctgccaag tccatcgctg cagccaccag tgcactggta
     6421 aaggctgcgt cagcagccca gagggagctg gtggctcaag gaaaggtggg cgccattcca
     6481 gccaatgcac tggatgatgg gcagtggtcg cagggcctca tttccgctgc ccgtatggtg
     6541 gctgcggcca ccaacaatct gtgtgaagca gctaacgcag ctgtccaggg ccatgctagc
     6601 caggagaaac tcatttcctc agccaagcaa gtagctgcct ccacagctca gctcctggta
     6661 gcttgcaagg tcaaagcaga tcaggactct gaggcaatga aacggctcca ggctgctggc
     6721 aatgcagtga agagggcctc agataacctg gtaaaggcgg cccagaaagc tgcagccttc
     6781 gaagaccagg agaatgagac ggtggtggtg aaggagaaaa tggttggggg cattgcccag
     6841 attatcgcag cacaggaaga gatgcttcgg aaggaacgag agctggaaga ggctcggaaa
     6901 aagctcgccc agatccggca gcagcagtac aagttcttgc cttcagagct tcgagacgag
     6961 cacatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     7021 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     7081 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     7141 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     7201 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     7261 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     7321 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     7381 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     7441 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     7501 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     7561 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     7621 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaaccgcgg
     7681 gcccgggatc caccggatct agataactga tcataatcag ccataccaca tttgtagagg
     7741 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     7801 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7861 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7921 tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa attcgcgtta
     7981 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     8041 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     8101 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     8161 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     8221 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     8281 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     8341 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca
     8401 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     8461 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     8521 aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     8581 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     8641 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     8701 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     8761 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     8821 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     8881 tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     8941 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     9001 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     9061 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
     9121 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     9181 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     9241 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     9301 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     9361 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     9421 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
     9481 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     9541 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     9601 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     9661 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     9721 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     9781 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     9841 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc
     9901 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa
     9961 gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
    10021 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
    10081 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
    10141 gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac
    10201 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    10261 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    10321 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    10381 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    10441 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    10501 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    10561 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    10621 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    10681 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    10741 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    10801 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    10861 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    10921 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    10981 ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcattag ttattaatag
    11041 taatcaatta cgg
//
