LOCUS       sequence-417763-       10401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus backbone expressing ATAD3A with an HA-tag at the 
            N-terminal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10401)
  AUTHORS   Fu Y, Sacco O, DeBitetto E, Kanshin E, Ueberheide B, Sfeir A
  TITLE     Mitochondrial DNA breaks activate an integrated stress response to 
            reestablish homeostasis.
  JOURNAL   Mol Cell. 2023 Oct 8:S1097-2765(23)00753-0. doi: 
            10.1016/j.molcel.2023.09.026.
  PUBMED    37832546
REFERENCE   2  (bases 1 to 10401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2023 Oct 8:S1097-2765(23)00753-0. doi: 
            10.1016/j.molcel.2023.09.026."
COMMENT     Sequence Label: pHAGE2-EF1a-HA-ATAD3A-IRES-Hygro
FEATURES             Location/Qualifiers
     source          1..10401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1009..1597
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1062..1082)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1480..1491)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1745..1925
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1972..1990)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2058..2162
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2163..2231,2232..3023)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2381..2400)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3194..3782
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3936..3953
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4193..4214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4229..4234,4235..4252,4253..4259)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4267..4283
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4272..4294
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4291..4307
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4291..4307
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4357..4376
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4568..5201
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5248..5373
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5870..6103
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6288..6332
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6625..6742
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6790..7968
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7021..7959
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7916..7936
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             8003..8029
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    9884..10345
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9940..9957)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     10167..10186
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(10350..10401,1..974)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             join(10350..10401,1..974)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
       61 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
      121 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
      181 ttgacattgg ggaatttagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
      241 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
      301 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
      361 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
      421 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
      481 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
      541 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
      601 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
      661 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
      721 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
      781 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
      841 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
      901 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
      961 gggcaaagga atagagtaga tgccgaccgg gatctatcga tagatcctaa tcaacctctg
     1021 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     1081 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     1141 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     1201 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     1261 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     1321 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     1381 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     1441 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     1501 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     1561 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac
     1621 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     1681 aattcactcc caacgaagac aagatcacct gcaggacagg cgcgccctgc tttttgcttg
     1741 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     1801 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     1861 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     1921 tagcacccgg gcgattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga
     1981 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     2041 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     2101 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     2161 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     2221 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     2281 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     2341 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     2401 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     2461 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     2521 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     2581 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     2641 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     2701 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     2761 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     2821 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     2881 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     2941 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     3001 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     3061 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     3121 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     3181 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3241 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3301 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3361 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3421 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3481 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3541 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3601 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3661 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3721 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3781 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3841 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3901 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     3961 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca
     4021 agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     4081 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     4141 cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     4201 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     4261 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt
     4321 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     4381 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
     4441 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
     4501 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
     4561 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
     4621 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     4681 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     4741 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     4801 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     4861 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     4921 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     4981 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     5041 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     5101 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     5161 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     5221 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     5281 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     5341 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     5401 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     5461 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     5521 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     5581 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     5641 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     5701 gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     5761 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     5821 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     5881 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     5941 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     6001 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag cttcaggcaa
     6061 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     6121 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     6181 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     6241 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     6301 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     6361 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     6421 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     6481 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     6541 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cgccgaattc
     6601 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     6661 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     6721 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggac
     6781 tagtcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     6841 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
     6901 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     6961 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     7021 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     7081 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
     7141 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
     7201 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
     7261 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
     7321 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
     7381 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
     7441 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
     7501 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
     7561 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
     7621 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
     7681 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
     7741 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     7801 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     7861 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     7921 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgaag cggccgctta
     7981 attaagttta aacggatcca tgtacccata cgatgttcca gattacgctt cgtggctctt
     8041 cggcattaac aagggcccca agggtgaagg cgcggggccg ccgccgcctt tgccgcccgc
     8101 gcagcccggg gccgagggcg gcggggaccg cgggttggga gaccggccgg cgcccaagga
     8161 caaatggagc aacttcgacc ccaccggcct ggagcgcgcc gccaaggcgg cgcgcgagct
     8221 ggagcactcg cgttatgcca aggacgccct gaatctggca cagatgcagg agcagacgct
     8281 gcagttggag caacagtcca agctcaaaga gtatgaggcc gccgtggagc agctcaagag
     8341 cgagcagatc cgggcgcagg ctgaggagag gaggaagacc ctgagcgagg agacccggca
     8401 gcaccaggcc agggcccagt atcaagacaa gctggcccgg cagcgctacg aggaccaact
     8461 gaagcagcag caacttctca atgaggagaa tttacggaag caggaggagt ccgtgcagaa
     8521 gcaggaagcc atgcggcgag ccaccgtgga gcgggagatg gagctgcggc acaagaatga
     8581 gatgctgcga gtggaggccg aggcccgggc gcgcgccaag gccgagcggg agaatgcaga
     8641 catcatccgc gagcagatcc gcctgaaggc ggccgagcac cgtcagaccg tcttggagtc
     8701 catcaggacg gctggcacct tgtttgggga aggattccgt gcctttgtga cagactggga
     8761 caaagtgaca gccacggtgg ctgggctgac gctgctggct gttggggtct actcagccaa
     8821 gaatgccacg cttgtcgccg gccgcttcat cgaggctcgg ctggggaagc cgtccctagt
     8881 gagggagacg tcccgcatca cggtgcttga ggcgctgcgg caccccatcc aggtcagccg
     8941 gcggctcctc agtcgacccc aggacgcgct ggagggtgtt gtgctcagtc ccagcctgga
     9001 agcacgggtg cgcgacatcg ccatagcaac aaggaacacc aagaagaacc gcagcctgta
     9061 caggaacatc ctgatgtacg ggccaccagg caccgggaag acgctgtttg ccaagaaact
     9121 cgccctgcac tcaggcatgg actacgccat catgacaggc ggggacgtgg cccccatggg
     9181 gcgggaaggc gtgaccgcca tgcacaagct ctttgactgg gccaatacca gccggcgcgg
     9241 cctcctgctc tttgtggatg aagcggacgc cttccttcgg aagcgagcca ccgagaagat
     9301 aagcgaggac ctcagggcca cactgaacgc cttcctgtac cgcacgggcc agcacagcaa
     9361 caagttcatg ctggtcctgg ccagcaacca accagagcag ttcgactggg ccatcaatga
     9421 ccgcatcaat gagatggtcc acttcgacct gccagggcag gaggaacggg agcgcctggt
     9481 gagaatgtat tttgacaagt atgttcttaa gccggccaca gaaggaaagc agcgcctgaa
     9541 gctggcccag tttgactacg ggaggaagtg ctcggaggtc gctcggctga cggagggcat
     9601 gtcgggccgg gagatcgctc agctggccgt gtcctggcag gccacggcgt atgcctccga
     9661 ggacggggtc ctgaccgagg ccatgatgga cacccgcgtg caagatgctg tccagcagca
     9721 ccagcagaag atgtgctggc tgaaggcgga agggcctggg cgtggggacg agccctcccc
     9781 atcctgagct agcgttaaca cgcgttgtac attttacgta tctagagctc gaggtcgacc
     9841 aattccggtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     9901 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     9961 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
    10021 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
    10081 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
    10141 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
    10201 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
    10261 ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
    10321 tttcctttga aaaacacgat gataatacca tgaaaaagcc tgaactcacc gcgacgtctg
    10381 tcgagaagtt tctgatcgaa a
//
