LOCUS       sequence-417815-       10470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDEST vector with TRP1 marker for gene expression in S. cerevisiae. 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10470)
  AUTHORS   Evans-Yamamoto D, Dube AK, Saha G, Plante S, Bradley D, 
            Gagnon-Arsenault I, Landry CR
  TITLE     Parallel nonfunctionalization of CK1delta/epsilon kinase ohnologs 
            following a whole-genome duplication event.
  JOURNAL   Mol Biol Evol. 2023 Nov 18:msad246. doi: 10.1093/molbev/msad246.
  PUBMED    37979156
REFERENCE   2  (bases 1 to 10470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Evol. 2023 Nov 18:msad246. doi: 10.1093/molbev/msad246."
COMMENT     Sequence Label: pDN0520 (pDEST, TRP1)
FEATURES             Location/Qualifiers
     source          1..10470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(682..929)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     895..913
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(2177..2195)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2216..2232)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2216..2232)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2229..2251)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2240..2256
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2264..2270,2271..2288,2289..2294))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2309..2330
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2447..2464)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2618..3206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2698..2717)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3377..4168,4169..4237))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4000..4019
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4238..4342)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    4379..4882
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     4923..4941
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4979..5001)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5101..5120
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5141..5421
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             5422..6096
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(6199..6654)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6336..6357)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6548..6567
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6780..6802
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6794..6811
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6795..6811
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6818..6837
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6818..6836
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(6989..7176)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     7097..7121
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    7755..7879
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(7920..8225)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8022..8041)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(8545..9225)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     9140..9159
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(9226..9328)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(9502..9626)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cataggagaa gtgtaaagtt cccttccata ttgtaacata ataaagtgca cacccaaatg
       61 aattgaaagc gtactcaaac agacaaccat ttccagtgtt gtatgtacct gtctatttat
      121 actggtagca accctattgc tgtttcctct tcaaagtact ctagcggtta tgcgcgtctc
      181 accttcaagg tcatggtcgc tctattgttc gcaccaccgg caaactcgcg tctcgcaagt
      241 cttggctcat tcttctagta tactcatgtt gcaaatgcac tcaggttctt tcggcaactt
      301 aaataatgac accagttgtc gtggtcgtca tcatcgcaac cccaaccggc attcttattg
      361 cttctccaat ctcgcccctt agcgcagggt aaaccttgga aaatgcaggc gcaaaaaact
      421 ccgccgggca cagcctcacg cccagcgtta tcgccgggcc ggcaagagcg cgggtccgcc
      481 acagagtcag catgattgtg caattgcgta aactcgtttt ttcggcgccg caaagccaaa
      541 tacatcatat caacactttt cactttattt ttcgttcgac ccttatattt gtcttttgcc
      601 ttcatgctcc ttgatttcct atttcattta ccatcatttc ttcgatcccg gatctcgagg
      661 gggggcccgg atcccgagct tgcaaattaa agccttcgag cgtcccaaaa ccttctcaag
      721 caaggttttc agtataatgt tacatgcgta cacgcgtctg tacagaaaaa aaagaaaaat
      781 ttgaaatata aataacgttc ttaatactaa cataactata aaaaaataaa tagggaccta
      841 gacttcaggt tgtctaactc cttccttttc ggttagagcg gatgtggggg gagggcgtga
      901 atgtaagcgt gacataacta attacatgat atcgacaaag gaaaaggggc ctgtttactc
      961 acaggctttt ttcaagtagg taattaagtc gtttctgtct ttttccttct tcaacccacc
     1021 aaaggccatc ttggtacttt tttttttttt tttttttttt tttttttttt tttttttttt
     1081 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
     1141 tttttttttt tttttttttt ttttgataga aataatacag aagtagatgt tgaattagat
     1201 taaactgaag atatataatt tattggaaaa tacatagagt ttttgttgat gcgcttaagc
     1261 gatcaattca acaacaccac cagcagctct gattttttct tcagccaact tggagacgaa
     1321 tctagctttg acgataactg gaacatttgg aattctaccc ttacccaaga tcttaccgta
     1381 accggctgcc aaagtgtcaa taactggagc agtttcctta gaagcagatt tcaagtattg
     1441 gtctctcttg tcttctggga tcaatgtcca caatttgtcc aagttcaaga ctggcttcca
     1501 gaaatgagct tgttgcttgt ggaagtatct cataccaacc ttaccgaaat aacctggatg
     1561 gtatttatcc atgttaattc tgtggtgatg ttgaccaccg gccatacctc taccaccggg
     1621 gtgctttctg tgcttaccga tacgaccttt accggctgag acgtgacctc tgtgctttct
     1681 agtcttagtg aatctggaag gcattcttga ttagttggat gattgttctg ggatttaatg
     1741 caaaaaaatc actaagaagg aaaaaaatca acggagaaag caaacgccat cttaaatata
     1801 cgggatacag atgaaaggtt tgaacctatc tgggaaaata cgcattaaac aagcgaaaaa
     1861 ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg cgccagagga aaaataggaa
     1921 aaataacagg cattagaaaa ataattttga ttttggtaat gtgtgggtcc ctggtgtaca
     1981 gatgttacat tggttacagt actcttgttt ttgctgtgtt tttcgatgaa tctccaaaat
     2041 ggttgttagc acatggaaga gtcaccgatg ctaagttatc tctatgtaag ctacgtggcg
     2101 tgacttttga tggagccgca caagagatac aggattggca actgcaaata gaatctgggg
     2161 atcccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat
     2221 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     2281 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     2341 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     2401 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     2461 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     2521 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2581 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2641 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2701 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2761 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2821 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2881 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2941 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3001 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     3061 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3121 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3181 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3241 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     3301 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3361 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3421 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     3481 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3541 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3601 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3661 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3721 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3781 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3841 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3901 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3961 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     4021 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4081 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4141 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4201 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4261 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4321 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tccttttcat
     4381 cacgtgctat aaaaataatt ataatttaaa ttttttaata taaatatata aattaaaaat
     4441 agaaagtaaa aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga tgtaaaagac
     4501 tctaggggga tcgccaacaa atactacctt ttatcttgct cttcctgctc tcaggtatta
     4561 atgccgaatt gtttcatctt gtctgtgtag aagaccacac acgaaaatcc tgtgatttta
     4621 cattttactt atcgttaatc gaatgtatat ctatttaatc tgcttttctt gtctaataaa
     4681 tatatatgta aagtacgctt tttgttgaaa ttttttaaac ctttgtttat ttttttttct
     4741 tcattccgta actcttctac cttctttatt tactttctaa aatccaaata caaaacataa
     4801 aaataaataa acacagagta aattcccaaa ttattccatc attaaaagat acgaggcgcg
     4861 tgtaagttac aggcaagcga tccgtctaag aaaccattat tatcatgaca ttaacctata
     4921 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     4981 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     5041 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     5101 cggcatcaga gcagattgta ctgagagtgc accatagatc acgacattac tatatatata
     5161 atataggaag catttaatag aacagcatcg taatatatgt gtactttgca gttatgacgc
     5221 cagatggcag tagtggaaga tattctttat tgaaaaatag cttgtcacct tacgtacaat
     5281 cttgatccgg agcttttctt tttttgccga ttaagaatta attcggtcga aaaaagaaaa
     5341 ggagagggcc aagagggagg gcattggtga ctattgagca cgtgagtata cgtgattaag
     5401 cacacaaagg cagcttggag tatgtctgtt attaatttca caggtagttc tggtccattg
     5461 gtgaaagttt gcggcttgca gagcacagag gccgcagaat gtgctctaga ttccgatgct
     5521 gacttgctgg gtattatatg tgtgcccaat agaaagagaa caattgaccc ggttattgca
     5581 aggaaaattt caagtcttgt aaaagcatat aaaaatagtt caggcactcc gaaatacttg
     5641 gttggcgtgt ttcgtaatca acctaaggag gatgttttgg ctctggtcaa tgattacggc
     5701 attgatatcg tccaactgca tggagatgag tcgtggcaag aataccaaga gttcctcggt
     5761 ttgccagtta ttaaaagact cgtatttcca aaagactgca acatactact cagtgcagct
     5821 tcacagaaac ctcattcgtt tattcccttg tttgattcag aagcaggtgg gacaggtgaa
     5881 cttttggatt ggaactcgat ttctgactgg gttggaaggc aagagagccc cgaaagctta
     5941 cattttatgt tagctggtgg actgacgcca gaaaatgttg gtgatgcgct tagattaaat
     6001 ggcgttattg gtgttgatgt aagcggaggt gtggagacaa atggtgtaaa agactctaac
     6061 aaaatagcaa atttcgtcaa aaatgctaag aaataggtta ttactgagta gtatttattt
     6121 aagtattgtt tgtgcacttg ccgatctatg cggtgtgaaa taccgcacag atgcgtaagg
     6181 agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     6241 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     6301 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     6361 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     6421 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     6481 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     6541 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     6601 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc
     6661 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     6721 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     6781 ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga
     6841 attgggatcc gtgtggaaga acgattacaa caggtgttgt cctctgagga cataaaatac
     6901 acaccgagat tcatcaactc attgctggag ttagcatatc tacaattggg tgaaatgggg
     6961 agcgatttgc aggcatttgc tcggcatgcc ggtagaggtg tggtcaataa gagcgacctc
     7021 atgctatacc tgagaaagca acctgaccta caggaaagag ttactcaaga ataagaattt
     7081 tcgttttaaa acctaagagt cactttaaaa tttgtataca cttatttttt ttataactta
     7141 tttaataata aaaatcataa atcataagaa attcgcttat ttagaagtgt caacaacgta
     7201 tctaccaacg atttgaccct tttccatctt ttcgtaaatt tctggcaagg tagacaagcc
     7261 gacaaccttg attggagact tgaccaaacc tctggcgaag aagtccaaag ctccaccgcg
     7321 gtggcggccg ttacttactt agagcttatt accctgttat ccctaaccca taatacccat
     7381 aatagctgtt tgccattact tacttaaatt aggtacccaa ttccggttgt tcaataagtc
     7441 ttaaggcatc atccaaactt ttggcaagaa aatgagctcc tcgtggtggt tctttgagtt
     7501 ctctactgag aactatatta attctgtcct ttaaaggtcg attcttctca ggaatggaga
     7561 accaggtttt cctacccata atcaccagat tctgtttacc ttccactgaa gaggttgtgg
     7621 tcattctttg gaagtacttg gactcgttcc tgagcggagg ccagggatag tctccgttct
     7681 tgccaatccc catattttgg gacacggcga cgatgcagtt caatggtcga accatactag
     7741 tacttcctcc tcctaccact ttgtacaaga aagctgaacg agaaacgtaa aatgatataa
     7801 atatcaatat attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac
     7861 aacatatcca gtcactatgg tcgacctgca gactggctgt gtataaggga gcctgacatt
     7921 tatattcccc agaacatcag gttaatggcg tttttgatgt cattttcgcg gtggctgaga
     7981 tcagccactt cttccccgat aacggagacc ggcacactgg ccatatcggt ggtcatcatg
     8041 cgccagcttt catccccgat atgcaccacc gggtaaagtt cacgggggac tttatctgac
     8101 agcagacgtg cactggccag ggggatcacc atccgtcgcc cgggcgtgtc aataatatca
     8161 ctctgtacat ccacaaacag acgataacgg ctctctcttt tataggtgta aaccttaaac
     8221 tgcatttcac cagcccctgt tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc
     8281 gacctcagcc atcccttcct gattttccgc tttccagcgt tcggcacgca gacgacgggc
     8341 ttcattctgc atggttgtgc ttaccagacc ggagatattg acatcatata tgccttgagc
     8401 aactgatagc tgtcgctgtc aactgtcact gtaatacgct gcttcatagc atacctcttt
     8461 ttgacatact tcgggtatac atatcagtat atattcttat accgcaaaaa tcagcgcgca
     8521 aatacgcata ctgttatctg gcttttagta agccggatcc tctagattac gccccgcctg
     8581 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     8641 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     8701 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     8761 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
     8821 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tatgtagaaa
     8881 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     8941 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     9001 catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     9061 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     9121 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     9181 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     9241 tcctgaaaat ctcgatgact caaaaaatac gcccggtagt gatcttattt cattatggtg
     9301 aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg
     9361 cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac
     9421 aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc aacttagcgg ccgctaagtt
     9481 ggcagcatca cccgcggccg ccatagtgac tggatatgtt gtgttttaca gtattatgta
     9541 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct
     9601 cgttcagctt ttttgtacaa acttgtttga ttcgacccac cctttgtatg cttggtatag
     9661 cttgaaatat tgtgcagaaa aagaaacaag gaagaaaggg aacgagaaca atgacgagga
     9721 aacaaaagat taataattgc aggtctattt atacttgata gcaagacagc aaactttttt
     9781 ttatttcaaa ttcaagtaac tggaaggaag gccgtatacc gttgctcatt agagagtagt
     9841 gtgcgtgaat gaaggaagga aaaagtttcg tgtgcttcga gatacccctc atcagctctg
     9901 gaacaacgac atctgttggt gctgtctttg tcgttaattt tttcctttag tgtcttccat
     9961 catttttttg tcattgcgga tatggtgaga caacaacggg ggagagagaa aagaaaaaaa
    10021 aagaaaagaa gttgcatgcg cctattatta cttcaataga tggcaaatgg aaaaagggta
    10081 gtgaaacttc gatatgatga tggctatcaa gtctagggct acagtattag ttcgttatgt
    10141 accaccatca atgaggcagt gtaattggtg tagtcttgtt tagcccatta tgtcttgtct
    10201 ggtatctgtt ctattgtata tctcccctcc gccacctaca tgttagggag accaacgaag
    10261 gtattatagg aatcccgatg tatgggtttg gttgccagaa aagaggaagt ccatattgta
    10321 cacccggaaa caacaaaagg atatccgaaa tattccacgg tttagaaaaa aatcggaaaa
    10381 gagcgcggag gggtgttacc ccccttctct actagcattg gactttaatt tcgctacctt
    10441 aggaccgtta tagttacatt aatatatgtg
//
