LOCUS       sequence-418204-        2137 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 0 promoter part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBP-P_dummy-promoter
FEATURES             Location/Qualifiers
     source          1..2137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(162..256)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(277..936)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     851..870
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1098..1116
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1120..1163)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1166..1185
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      1276..1333
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1357..1374)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1528..2116)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1608..1627)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
       61 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
      121 agtatatatg agtaaacttg gtctgacagc tcgaggcttg gattctcacc aataaaaaac
      181 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
      241 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
      301 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
      361 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
      421 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
      481 actcacccag ggattggctg acacgaaaaa catattctca ataaaccctt tagggaaata
      541 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
      601 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
      661 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
      721 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
      781 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
      841 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
      901 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
      961 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     1021 acctcttacg tgcccgatca actcgagtgc cacctgacgt ctaagaaacc attattatca
     1081 tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct
     1141 tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagaatct agagacgcat
     1201 aggtctcact atgcatggcg gtcgtatcga ggtcttgagg tggtacagag acccatgccg
     1261 tctcattagc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt
     1321 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc
     1381 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1441 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1501 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
     1561 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1621 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1681 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1741 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1801 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1861 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1921 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1981 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2041 caaacaaacc accgctacca gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2101 aaaaaaagga tctcaagaag atcctttgat cttttct
//
