LOCUS       sequence-418238-        2352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 0 CDS part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBP-C_ccdB
FEATURES             Location/Qualifiers
     source          1..2352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(172..266)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(287..946)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     861..880
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1108..1126
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1130..1173)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             1184..1489
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1368..1387
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     terminator      1501..1558
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1582..1599)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1753..2341)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1833..1852)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
       61 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      121 atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgaggcttg gattctcacc
      181 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
      241 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
      301 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
      361 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
      421 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
      481 aactggtgaa actcacccag ggattggctg acacgaaaaa catattctca ataaaccctt
      541 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
      601 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
      661 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
      721 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
      781 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
      841 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
      901 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
      961 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     1021 gaaagttgga acctcttacg tgcccgatca actcgagtgc cacctgacgt ctaagaaacc
     1081 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa
     1141 aaaaaatcct tagctttcgc taaggatgat ttcggtctca catatgcagt ttaaggttta
     1201 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga
     1261 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt
     1321 ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac
     1381 cgatatggcc agtgtgccgg tgtccgttat cggggaagaa gtggctgatc tcagccaccg
     1441 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataat cgaagagacc
     1501 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
     1561 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1621 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1681 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1741 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1801 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1861 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1921 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1981 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2041 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2101 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2161 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2221 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2281 taccagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2341 agaagatcct tt
//
