LOCUS       sequence-418251-        2099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 0 terminator part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBP-T_L3S1P47
FEATURES             Location/Qualifiers
     source          1..2099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(187..281)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(302..961)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     876..895
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1123..1141
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1145..1188)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1263..1320
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1344..1361)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(1515..2099,1..4))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1595..1614)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
       61 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
      121 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag
      181 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
      241 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
      301 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
      361 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
      421 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
      481 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgacacg aaaaacatat
      541 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
      601 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
      661 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
      721 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
      781 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
      841 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
      901 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
      961 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     1021 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaactcg agtgccacct
     1081 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     1141 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttcgg tctcatcgat
     1201 tttcgaaaaa aggcctccca aatcgggggg ccttttttta tagcaacaaa atgttagaga
     1261 cctccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga
     1321 ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1381 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     1441 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1501 gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1561 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1621 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1681 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1741 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1801 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1861 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1921 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     1981 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2041 gctaccagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatc
//
