LOCUS       sequence-41867-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00090
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(125..142)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(611..628)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa tgctttctta
       61 taatgccaac tttgtacaag aaagttggca ataagcccgt ggctccagcg gccatcattc
      121 cgccgtgatg atggtggtga tgcattccgc cgttctggaa gtacatgtag tagttgtacg
      181 gcgactgctg tcccgcccca ccctcgcagt cgagcagtgg ctgaccaccg gccgttcccg
      241 ccaccggcga aggagtcatc ggcagcggtg ataccgtact gcccatgccg cccattccac
      301 ccactccgac gcccacctgg caactggcgc gcagggcaac agcctgatgg tgggccagga
      361 tatcgctgac cgagtgggag gagggccagt gggcggcagc ggctgcggcg gccacactcc
      421 tcagctggtg gggatggggc actgatccct gaccgcccgc tccctgcggc ggggatggac
      481 ttccacaggg cgtcttcatg ctgtaggccg ccgcagcact gtacggctgg tagatggagt
      541 tgtacaggtg ggcgtgggca tgggcgtggg cgtgcacatg atgggcactt gccgccgccg
      601 ccgctgactg atgatgatga tggtggtgcg gatggtgcgg tccaccgccg ggattaccca
      661 gattgctcag attaatatta ttgctggcac tcgtgccatt gttttgtcca ccattgctgg
      721 acacactgcc gccaccgctg ctgcttccgc tgcccatcac cgttcccggc gtgtgctgat
      781 gccccaggct
//
