LOCUS       sequence-418724-        8650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing gRNA targeting GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Aoki K, Hyuga M, Tarumoto Y, Nishibuchi G, Ueda A, Ochi Y, Sugino S,
            Mikami T, Kobushi H, Kato I, Akahane K, Inukai T, Takaori-Kondo A, 
            Takita J, Ogawa S, Yusa K
  TITLE     Canonical BAF complex regulates the oncogenic program in human 
            T-cell acute lymphoblastic leukemia.
  JOURNAL   Blood. 2023 Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857.
  PUBMED    37922452
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2023
            Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857."
COMMENT     Sequence Label: pKLV2-U6gRNA5(GFP)-PGKpuro2ABFP-W
FEATURES             Location/Qualifiers
     source          1..8650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        270..468
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     423..443
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             486..666
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    713..838
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1337..1570
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1755..1799
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2097..2214
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2289..2537
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2289..2309
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2460..2479
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2666..3165
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2701..2724)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3098..3117
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3205..3801
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3205..3224)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3701..3721
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3820..3873
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3874..4575
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(4635..4655)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5053..5064)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5516..5533)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5522..5746
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5772..5792)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5772..5790)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5811..5827)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5811..5827)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5824..5846)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5835..5851
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5859..5865,5866..5883,5884..5889))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5904..5925
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6042..6059)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6213..6801)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6293..6312)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6972..7763,7764..7832))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7595..7614
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7833..7937)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7963..8418)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8100..8121)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8312..8331
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8545..8567
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8559..8576
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8560..8576
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8586..8605
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8586..8604
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
       61 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      121 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      181 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      241 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      301 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      361 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      421 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct
      481 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
      541 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
      601 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
      661 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
      721 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
      781 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
      841 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
      901 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
      961 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     1021 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     1081 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     1141 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgcgctg atcttcagac
     1201 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     1261 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     1321 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     1381 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     1441 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     1501 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     1561 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     1621 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     1681 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     1741 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     1801 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     1861 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     1921 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     1981 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     2041 acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt
     2101 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     2161 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     2221 tattacaggg acagcagaga tccagtttgg ttagtaccgg gccctacgcg tccaaggtcg
     2281 ggcaggaaga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
     2341 ttagagagat aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt
     2401 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
     2461 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2521 ggaaaggacg aaacaccgaa gttcgagggc gacacccgtt taagagctat gctggaaaca
     2581 gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
     2641 tgcttttttt ggatccaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     2701 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
     2761 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
     2821 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
     2881 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
     2941 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
     3001 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
     3061 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
     3121 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg catccatcta
     3181 gatctcgagc agctgaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
     3241 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     3301 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     3361 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     3421 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     3481 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     3541 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     3601 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     3661 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     3721 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     3781 atgacccgca agcccggtgc cggcggcggg tccggaggag agggcagagg aagtctccta
     3841 acatgcggtg acgtggagga gaatcctggc ccaatgagcg agctgattaa ggagaacatg
     3901 cacatgaagc tgtacatgga gggcaccgtg gacaaccatc acttcaagtg cacatccgag
     3961 ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc
     4021 cctctcccct tcgccttcga catcctggct actagcttcc tctacggcag caagaccttc
     4081 atcaaccaca cccagggcat ccccgacttc ttcaagcagt ccttccctga gggcttcaca
     4141 tgggagagag tcaccacata cgaggacggg ggcgtgctga ccgctaccca ggacaccagc
     4201 ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt cacatccaac
     4261 ggccctgtga tgcagaagaa aacactcggc tgggaggcct tcaccgagac gctgtacccc
     4321 gctgacggcg gcctggaagg cagaaacgac atggccctga agctcgtggg cgggagccat
     4381 ctgatcgcaa acatcaagac cacatataga tccaagaaac ccgctaagaa cctcaagatg
     4441 cctggcgtct actatgtgga ctacagactg gaaagaatca aggaggccaa caacgagacc
     4501 tacgtcgagc agcacgaggt ggcagtggcc agatactgcg acctccctag caaactgggg
     4561 cacaagctta attgagcggc cgctcaacct ctggattaca aaatttgtga aagattgact
     4621 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     4681 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     4741 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     4801 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     4861 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     4921 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     4981 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     5041 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     5101 ctgcggcctc ttccgcgtct acgccttcgc cctcagacga gtcggatctc cctttgggcc
     5161 gcctccccgc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     5221 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaaagaag tcaagatctg
     5281 ctttttgcct gtactgggtc tctctggtta gaccagagtc tctctggtta gaccagatct
     5341 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5401 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5461 tcagaccctt ttagtcagtg tggaaaatct ctagcagttt aaacccgctg atcagcctcg
     5521 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     5581 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     5641 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     5701 tgggaagtca atagcaggca tgctggggat gcggtgggct ctatgggcgg ccgttaatta
     5761 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
     5821 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cgggagcata
     5881 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     5941 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6001 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6061 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6121 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     6181 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6241 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6301 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6361 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6421 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6481 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6541 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     6601 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     6661 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     6721 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     6781 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     6841 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     6901 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     6961 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7021 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     7081 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     7141 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     7201 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     7261 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     7321 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     7381 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     7441 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     7501 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     7561 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     7621 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     7681 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     7741 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     7801 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     7861 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     7921 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
     7981 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     8041 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     8101 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     8161 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     8221 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     8281 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     8341 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     8401 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     8461 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     8521 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
     8581 gcgcgtaata cgactcacta tagggcgaat tgactagtac ataacttacg gtaaatggcc
     8641 cgcctggctg
//
